首页 > GenWay > GenWay/Coxiella Burnetii (Q-Fever) Phase 2 IgG/GWB-2EB8D1/1x96 Assays

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GenWay/Coxiella Burnetii (Q-Fever) Phase 2 IgG/GWB-2EB8D1/1x96 Assays

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GWB-2EB8D1

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Description:ProductNameCoxiellaburnetii(Q-Fever)Phase2IgG-ELISA.#ofSamples1x96Assays.IntendedUseTheELISAisintendedforthequalitativedeterminationofIgGclassantibodiesagainstCoxiellaburnetiiphase2intheearlystagesofinfectioninhumanserum.IntroductionQ-Feverisadiseasethatresultsfrominfectionwithsmall,polymorphandgram-negativebacteriacalledCoxiellaburnetii.AfteranoutbreakinBrisbane,Australia,theresponsIBLeorganismwasisolatedandnamedCoxiellaburnetiiinhonourofDr.HeraldRaeCoxandSirFrankBurnet.NewmolecularresearchdemonstratedacloserelationshiptoLegionella.ThezoonosisQ-FeverisfoundeverywhereexceptNewZealand(nodataavailable).Thereisanextensivereservoir(mainlyticks)ofC.burnetii.Ticksareanimportantvectorofthepathogeninthetransmissionbetweendomesticandwildlifeanimals.Buttheticksareunimportantinthedirectinfectionofhumans.Cattles,sheepandgoatsareusuallythesourceoftransmissionofthismicroorganismtohumans.Howevercats,dogsandrabbitsarealsoimportantinthisregard.InmostinstanceshumansbecomeinfectedwithCoxiellaburnetiifollowinginhalationofcontaminatedaerosols(respiratorytract).TheincubationperiodforQ-Feverinhumansisabout2weeks.Theresultingillnesscanbedividedintoacuteandchronicvarieties.Duringtheacutephaseofillnessantibodiestothephase2-antigenareformed.Antiphase-1antibodiesinhightitersaretypicalforachronicdisease.InareaswhereQ-Feverisendemic,12%ormoreofthepopulationhaveantibodiestoC.burnetii.Mostoftheinfectionsaresubclinicalorundiagnosed.Theacuteinfectionshowssymptomsofhighfever,shivers,musclepainandheadache.Lateronmoreseverediseasessuchaspneumoniaorhepatitiscanoccur.Infectionsduringpregnancycanleadtoanabortorprematurebirth.Approximately1%ofallinfectionsbecomechronic.ThemostfrequentorganmanifestationinQ-Feverisendocarditis.DiagnosticmethodsComplementbindingreactionisstillusedIFT(immunofixationtest)ELISACellculturePCRIFTaswellasELISAdifferentiatebetweendifferentantibodyclasses(IgG/IgM/IgA).AcutephaseofQ-FeverIgMspecifictophase2after2-3weeksIgGapproximately2monthsafterinfection.ChronicphaseofQ-FeverFrom6weeksupto4monthsafterinfectionphase1IgGandIgAantibodiescanbedetected.PrinciplesoftheassayThequalitativeimmunoenzymaticdeterminationofIgG-classantibodiestoCoxiellaburnetiiisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.Microtiterstripwellsareprecoatedwithphase2antigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgGconjugateisadded.ThisconjugatebindstothecapturedCoxiellaburnetii-specificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.Theintensityofthisproductisproportionaltotheamountofphase2-specificIgGantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlityThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2-8°C.LimitationsoftheTestBacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasSEROlogicaldata.Inimmunocompromisedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.ReferencesMcDadeJE:HistoricalaspectsofQ-Fever:Thedisease.CRCPress,1990,5MarrieTS:Coxiellaburnetiipneumonia.ClinInfectDisease21(Suppl.3),1995,253RaoultDandMarrieT:Q-Fever.ClinInfectDisease20,1995,489KaplanMMandBertagna,P:ThegeographicaldistributionofQ-Fever.BullWorldHealthOrgan13,1995,829DellacasagrandeJ,etal.:C.burnetiisurvivesinmonocytesfrompatientswithQ-Feverendocarditis.InfectImmun68,2000,160.AdditionalInformation:NameCoxiellaBurnetii(Q-Fever)Phase2IgGRelatedProductNamesCoxiellaburnetii(Q-Fever)Phase2IgG-ELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.IntendedUseResearchUseOnly

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