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GenWay/Toxocara canis IgG/GWB-3570CC/1x96 Assays
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Description:PleaseclickhereforMSDSPDFDatasheetProductName:ToxocaracanisIgG-ELISA#ofSamples:1x96AssaysIntendedUse:TheToxocaracanisIgG-ELISAisintendedforthequalitativedeterminationofantibodiesagainstToxocaracaniscirculatinginhumanserumorplasma(citrate).Introduction:Toxocaracanis(anascarid)isaparasiticnematode(roundworm)commonlyfoundintheintestineofdogs.Humansareparatenichostswhobecomeinfectedbyingestinginfectiveeggsincontaminatedsoil.Afteringestion,theeggsyieldlarvaethatpenetratetheintestinalwallandarecarriedbythecirculationtoawidevarietyoftissues(liver,heart,lungs,brain,muscle,eyes).Whilethelarvaedonotundergoanyfurtherdevelopmentinthesesites,theycancauseseverallocalreactionsthatarethebasisoftoxocariasis.Inmostcases,Toxocarainfectionsarenotserious,andmanypeople,especiallyadultsinfectedbyasmallnumberoflarvae(immatureworms),maynotnoticeanysymptoms.Themostseverecasesarerare,butaremorelikelytooccurinyoungchildren,whooftenplayindirt,oreatdirtcontaminatedbydogstool.ThetwomainclinicalpresentationsoftoxocariasisareOcularLarvaMigrans(OLM),aneyediseasethatcancauseblindness(eachyearmorethan700peopleinfectedwithToxocaraexperiencepermanentpartiallossofvision),andVisceralLarvaMigrans(VLM),adiseasethatcausesswellingofancillarybody’sorgansorcentralnervoussystem.Thepresenceofparasiteresp.infectionmaybeidentifiedbyMicroscopySEROlogy:DetectionofantibodiesbyELISAPrinciplesoftheassay:ThequalitativeimmunoenzymaticdeterminationofantibodiesagainstToxocaracanisisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.MicrotiterstripwellsareprecoatedwithsyntheticToxocaracanisantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledProteinAConjugateisadded.ThisconjugatebindstothecapturedToxocaracanisspecificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofToxocaracanisspecificantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.LimitationsoftheTest:CrossreactivitywithantibodiesagainstAscarislumbricoidesandSchistosomacannotbeexcluded.Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.Inimmunocompromisedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.ReferencesGlickman,L.,Schantz,P.,Grieve,R.„Toxocariasis“.ImmunodiagnosisofParasiticDiseases.Vol.1,HelminthicDiseases.Ed.WallsandSchantz.Academicpress,1986.pp.201-231.Schantz,P.ToxocaraLarvaMigransNow.AmJTropMedHyg.Vol.41(Sup3),1989,pp.21-34.Jacquier,P.etal.Immunodiagnosisoftoxocariasisinhumans:EvaluationofaNewEnzyme-linkedImmunosorbentAssayKit.J.Clin.Micro.Vol.29#9,Sept.1991,pp.1831-1835.Carlier,Y.etal.TheuseofanExcretory-SecretoryAntigenforanELISASpecificSerodiagnosisofVisceralLarvaMigrans.Biomedicine.Vol.36,1982,pp.39-42.Brunello,F.Falagiani,P.,Genchi,C.EnzymeImmunoassay(ELISA)fortheDetectionofSpecificIgGAntibodiestoToxocaracanisESAntigens.Boll.Ist.sieroter.Milan.Vol.65#1,1986,pp.54-60.Josephson,S.L.Toxocariasis.LaboratoryDiagnosisofInfectiousDiseases-PrinciplesandPractices,Vol.1,1988,pp.993-997.AdditionalInformation:NameToxocaracanisIgGRelatedProductNamesToxocaracanisIgG-ELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.IntendedUseResearchUseOnly

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