Description:1.IntendedUse TheGenWayHerpes1/2IgGAntibodyELISATestKithasbeendesignedforthethedetectionand thequantitativedeterminationofspecificIgGantibodiesagainstHerpes1/2inserumandplasma. FurtherapplicationsinotherbodyfluidsarepossIBLeandcanberequestedfromtheTechnical ServiceofGenWay. Thisassayisintendedforresearchuseonly. 2.GeneralInformation TheHerpessimplexvirustype1isanubiquitouspathogenofhumansthatusuallycauseseitherasymptomaticinfectionormildskinandmucosaldiseases.AntibodiestoHSV1occurinabout90% ofadults.NormallyHSV1istransmittedbyoralsecretionsoropenwoundspriortotheageoffive. Recentlyinadultsprimaryinfectionswereobserved,too.Aftertheprimaryinfectionsomeviruses establishalatentstateintheirhostcells(mostlyganglialcells).ThevirusDNAisintegratedinto thegenomeofthehostcell,whereitremainsuntiltheinfectedpersondies.Afterstimulationofthe hostcell,recurrentinfectionoccurs,whichiscalledanexacerbation,whenclinicalsymtomsappear. Therecurrencemaybecausedbydifferentkindsoftraumas,asfeverorphysiologicalchangesand diseases.Immunosuppressedpersonsmayshowasevereclinicalcourse.HSV1causesdifferent clinicalsymptomsinabout10%oftheprimaryinfections.HSV1causes85%andHSV215%of oralprimaryinfections.ThemajorclinicalmanifestationsassociatedwithHSV1infectionsare gingivostomatitis,keratitis,conjunctivitis,vesiculareruptionsoftheskin,encephalitis,eczemaand somelethalinfectionsofnewborns.Insome casesHSV1infectionleadstoameningitiswith differentneurologicalsymptoms.PersonsatanincreasedriskforseriousorprolongedHSV infectionsarethosewitheczema,severeburnsoradefectintheircell-mediatedimmunity.Thedrug AcycloviristhetreatmentofchoiceformostseriousHSVinfections.Thecommonmanifestations ofHSVinfectionsaresotypicalthattheinfectionmaybeeasilydiagnosedonclinicalrecognition alone.The"goldstandard"fordiagnosisofHSVinfectionremainsisolationofthevirusintissue culture.FortypingHSV1/HSV2Westernblotsorindirectimmunofluorescencemaybeperformed. DiagnosisoftheprimaryinfectionbyHSV1/HSV2canbeconfirmedbyasignificantriseofthe IgGtiterwithin6to10days.AfinishedinfectioncanbemonitoredbytheIgGELISA.Incaseofa sUSPicionofHSVencephalopathyitisrecommendedtoperformaparalleldeterminationofboth HSV-specificantibodies(IgGandIgM)inserumandliquor. 3.PrincipleoftheTest TheGenWayHerpes1/2IgGantibodytestkitisbasedontheprincipleoftheenzymeimmunoassay (EIA).Herpes1/2antigenisboundonthesurfaceofthemicrotiterstrips.Dilutedsampleserumor ready-to-usestandardsarePipettedintothewellsofthemicrotiterplate.AbindingbetweentheIgG antibodiesoftheserumandtheimmobilizedHerpes1/2antigentakesplace.Afteraonehour incubationatroomtemperature,theplateisrinsedwithdilutedwashsolution,inordertoremove unboundmaterial.Thenready-to-useanti-human-IgGperoxidaseconjugateisaddedandincubated for30minutes.Afterafurtherwashingstep,thesubstrate(TMB)solutionispipettedandincubated for20minutes,inducingthedevelopmentofabluedyeinthewells.Thecolordevelopmentis terminatedbytheadditionofastopsolution,whichchangesthecolorfrombluetoyellow.The resultingdyeismeasuredspectrophotometricallyatthewavelengthof450nm.Theconcentration oftheIgGantibodiesisdirectlyproportionaltotheintensityofthecolor. AdditionalInformation:NameHerpes1/2IgGELISARelatedProductNamesHerpes1/2IgGELISAIntendedUseResearchUseOnly