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GenWay/Chlamydia pneumoniae IgA/GWB-36BA84/1x96 Assays

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GWB-36BA84

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Description:PleaseclickhereforMSDSPDFDatasheetProductName:ChlamydiapneumoniaeIgAELISA#ofSamples:1x96AssaysIntendedUse:TheChlamydiapneumoniaeIgA-ELISAisintendedforthequalitativedeterminationofIgAclassantibodiesagainstChla-mydiapneumoniaeinhumanserumorplasma(citrate).Introduction:Chlamydiaearenomotile,Gramnegativeandobligatoryintracellulargrowingbacteriawhichformcharacteristicinclusionswithinthecytoplasmofparasitizedcells.TheyareeasilyvisIBLeinthelightmicroscope.ThreedifferentChlamydiaspeciespathogenicforhumansareknown:Chlamydiatrachomatis,ChlamydiapneumoniaeandChlamydiapsittaci,andonespeciesonlypathogenicforanimals(C.pecorum).Chlamydiatrachomatisisthemostprevalentagentofsexuallytransmitteddiseasesworldwide(400-500millioncases)andthenumberofinfectionsisconstantlygrowing.PregnantwomeninfectedwithC.trachomatismaytransmitthesebacteriaduringchildbirth,causingconjunctivitisorpneumoniainnewborns.Untreatedcasesofchlamydialinfectioncanleadtochronicsalpingitis,possiblyresultinginectopicpregnancyorinfertility.Inmales,C.trachomatisisamajorcauseofnon-gonococcalurethritis.AsevereprobleminChlamydiainfectionsisthefrequentasymptomaticinsidiouscoursewhichmayresultintheinitiationofchronicdiseases.Inmanyinstancesprimaryinfectionsarenotrecognizedandonlythesequelaecausedbyascended,persistingagentsarediagnosed.InfectionmaybeidentifiedbyMicroscopy:GiemsastainPCRSEROlogy:DetectionofantigensbyELISADetectionofantibodiesbyIF,EIA,ELISAPrinciplesoftheassay:ThequalitativeimmunoenzymaticdeterminationofIgA-classantibodiesagainstChlamydiapneumoniaeisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.MicrotiterstripwellsareprecoatedwithChlamydiapneumoniaeantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgAconjugateisadded.ThisconjugatebindstothecapturedChlamydiapneumoniae-specificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofChlamydiapneumoniae-specificIgAantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.Inimmunsuppremizedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.TheChlamydiapneumoniaeantigenwhichiscoatedontheplatesiscomprisedofelementarybodies.AcrossreactionwithChlamydiatrachomatiscannotbeexcludedwithseracontainingantibodiestoLPSandMOMP.ReferencesHoymeU.B.,SpitzbartH.(1996).PastandcurrentprevalenceofChlamydiatrachomatisinwomeninGermany.In:ChlamydiaResearch.AngelikaStary(ed.).ProceedingsofthethirdmeetingoftheEuropeanSocietyforChlamydiaResearch,Vienna,Austria,11.-14.Sept.p.391.PaavonenJ.(1996).Chlamydiatrachomatis:Amajorcauseofmucopurulentcervicitisandpelvicinflammatorydiseaseinwomen.In:SexuallyTransmittedDiseases.AdvancesinDiagnosisamdTreatment.Curr.Probl.Dermatol.ElsnerP.,EichmannA.(eds.),Basel,Karger,Vol.24,pp.110-122.PetersenE.E.,CladA.(1995).GenitaleChlamydieninfektionen.DeutschesÄrzteblatt92,Heft5,A-277-282.WeströmL.(1996).ConsequencesofgenitalChlamydiainfectionsinwomen.In:ChlamydiaResearch.AngelikaStary(ed.).ProceedingsofthethirdmeetingoftheEuropeanSocietyforChlamydiaResearch,Vienna,Austria,11.-14.Sept.pp.137-140.WeströmL.V.(1996).Chlamydiaanditseffectonreproduction.J.Brit.Fertil.Soc.1:23-30.AdditionalInformation:NameChlamydiapneumoniaeIgARelatedProductNamesChlamydiapneumoniaeIgA–ELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.IntendedUseResearchUseOnly

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