首页 > GenWay > GenWay/Progesterone Saliva ELISA/GWB-373DFD/1x96 Assays

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GenWay/Progesterone Saliva ELISA/GWB-373DFD/1x96 Assays

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GWB-373DFD

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GenWay

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4000-520-616

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Description:ProductName:ProgesteroneSaliva#ofSamples:1x96AssaysIntendedUse:CompetitiveimmunoenzymaticcolorimetricmethodforquantitativedeterminationofProgesteroneinsaliva.Introduction:ProgesteroneisaC-21steroidhormoneinvolvedinthefemalemenstrualcycle,pregnancy(supportsgestation)andembryogenesisofhumansandotherspecies.Progesteroneisthemajornaturallyoccurringhumanprogestagen.Progesteroneisimportantforaldosterone(mineralocorticoid)synthesis,as17-hydroxyprogesteroneisforcortisol(glucocorticoid).Progesteronelevelsarerelativelylowinchildrenandpostmenopausalwomen.Adultmaleshavelevelssimilartothoseinwomenduringthefollicularphaseofthemenstrualcycle.Inwomen,progesteronelevelsarerelativelylowduringthepreovulatoryphaseofthemenstrualcycle,riseafterovulation,andareelevatedduringthelutealphase.Ifpregnancyoccurs,progesteronelevelsaremaintainedatluteallevelsinitially.Afterdeliveryoftheplacentaandduringlactation,progesteronelevelsareverylow.Thefallinprogesteronelevelsfollowingdeliveryisoneofthetriggersformilkproduction.Progesteroneisproducedintheadrenalglands,thegonads(specificallyafterovulationinthecorpusluteum),thebrain,and,duringpregnancy,intheplacenta.Progesteroneconvertstheendometriumtoitssecretorystagetopreparetheuterusforimplantation.Ifpregnancydoesnotoccur,progesteronelevelswilldecrease,leADIng,inthehuman,tomenstruation.Progesteronebelongstothegroupofneurosteroidsthatarefoundinhighconcentrationsincertainareasinthebrainandaresynthesizedthere.Neurosteroidsaffectsynapticfunctioning,areneuroprotective,andaffectmyelinization.Progesteronehasmultipleeffectsoutsideofthereproductivesystem.ProgesteroneisThermogenic,itreducesspasmandrelaxessmoothmuscle.Bronchiarewidenedandmucusregulated.Progesteroneactsasanantiinflammatoryagentandregulatestheimmuneresponse.Progesteronealsoassistsinthyroidfunction,inbonebuildingbyosteoblasts.MeasurementofserumprogesteroneconcentrationshavebeenusedinevaluatingovarianfunctionPrinciplesoftheassay:Microtiterstripwellsareprecoatedwithanti-Progesteroneantibodies(solid-phase).ProgesteroneinthesamplecompeteswithaddedhorseradishperoxidaselabelledProgesterone(enzyme-labelledantigen)forantibodybinding.Afterincubationabound/freeseparationisperformedbysolid-phasewashing.Theimmunecomplexformedbyenzyme-labelledantigenisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisinverselyproportionaltotheamountofProgesteroneinthesample.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorptionat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°Cinthedark.LimitationsoftheTest:Sample(s),whicharecontaminatedmicroBIOLOGically,shouldnotbeusedintheassay.ItisimportantthatthetimeofreactionineachwellisheldconstantforreproducIBLeresults.Pipettingofsamplesshouldnotextendbeyondtenminutestoavoidassaydrift.Ifmorethanoneplateisused,itisrecommendedtorepeatthedoseresponsecurve.Additionofthesubstratesolutioninitiatesakineticreaction,whichisterminatedbytheadditionofthestopsolution.Therefore,theadditionofthesubstrateandthestoppingsolutionshouldbeaddedinthesamesequencetoeliminateanytimedeviationduringreaction.Platereadersmeasurevertically.Donottouchthebottomofthewells.Failuretoremoveadheringsolutionadequatelyintheaspirationordecantationwashstep(s)mayresultinpoorreplicationandspuriousresults.ReferencesWisdomG.B.(1976)Clin.Chem.22(8),12431255.DeVillaG.O.etal.(1972)J.Clin.Endoc.Metab.35,458.JoyceB.G.,GrahamF.R.andFahmyD.R.(1977)Steroids29(6),761.WinkelP.,GaedeP.andLyngbyeJ.(1976)J.Clin.Chem.22(4),422.RajkowskiK.N.,CittanovaN.,DesfossesB.andJayleM.F.(1977)Steroids29(5),LuY.,BentleyG.R.andGannP.(1999)FertilityandSterility71(5),863.AdditionalInformation:NameProgesteroneSalivaELISARelatedProductNamesProgesteroneSalivaELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°Cinthedark.IntendedUseResearchUseOnly

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