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GenWay/Respiratory syncytial Virus IgA/GWB-3BA5F4/1x96 Assays

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Description:PleaseclickhereforMSDSPDFDatasheetProductName:RespiratorysyncytialVirusIgAELISA#ofSamples:1x96AssaysIntendedUse:TheRSVIgA-ELISAisintendedforthequalitativedeterminationofIgAclassantibodiestoRespiratorySyncytialVirusinhumanserumorplasma(citrate).Introduction:Respiratorysyncytialvirus(RSV)isanegative-sense,envelopedRNAvirusandbelongstothefamilyofparamyxoviridae.RSVinfectionisadiseaseoftherespiratorytractwithvariouscharacteristics.Infectionofchildrencausesseriousbronchiolotiswhileadultsgetonlymildinfectionsoftheupperrespiratorysystem.Seriouscomplicationsarefrequentlyobservedinspecialriskgroupslikeprematureinfants,bABIes,olderpeopleandpersonswithchroniclediseasesoftherespiratorytract.Respiratorysyncytialvirusinfectscellsoftheepitheliaoftheupperrespiratorytractundcausesnecrosisbycellfusion,incombinationwithinflammatoryexudatesandblockageoftherespiratorysysteminseriousproblemscanoccur.IfthevirusgoesdowntothelowerrespiratorytractformationofoedemaandaveolarcollapsearepossIBLe.Thevirusisspreadallovertheworldandhighlycontagious.MostinfantsbecomeinfectedwithRSVintheirfirstwinterseason;between25%and40%havesignsorsymptomsofbronchiolitisorpneumonia,and0.5%to2%requirehospitalization.MostchildrenwillhaveSEROlogicalevidenceofRSVinfectionby3yearsofage.RSValsocausesrepeatedinfectionsthroughoutlife,usuallyassociatedwithmoderate-to-severecold-likesymptoms.Thepresenceofvirusresp.infectionmaybeidentifiedbyMicroscopy:IFSerology:DetectionofantibodiesbyELISAPrinciplesoftheassay:ThequalitativeimmunoenzymaticdeterminationofIgA-classantibodiestoRespiratorySyncytialVirus(RSV)isbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.MicrotiterstripwellsareprecoatedwithRespiratorySyncytialVirus(RSV)antigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgAconjugateisadded.ThisconjugatebindstothecapturedRespiratorySyncytialVirus(RSV)specificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofRespiratorySyncytialVirus(RSV)specificIgAantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStability:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.Inimmunocompromisedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.ReferencesWiegand,R.:RespiratorySyncytialVirus(RSV).In:Brandis,H.,W.Köhler,H.J.Eggers,G.Pulverer(ed.):Med.MikroBIOLOGie1994.GustavFischerVerlagStuttgart,Jena,NewYork(1994)767-769Wiegand,R.:RespiratorySyncytialVirus(RSV).In:Brandis,H.,W.Köhler,H.J.Eggers,G.Pulverer(ed.):Med.Mikrobiologie1994.GustavFischerVerlagStuttgart,Jena,NewYork(1994)767-769Hall,C.B.,etImmunitytoandfrequencyofreinfectionwithRespiratorySyncytialVirus.J.Infect.Dis.163(1991)693-698Hohlberg,C.J.,etal.:RiskfactorsforRespiratorySyncytialVirusassociatedlowerrespiratoryillnessintheyearoflife.Am.J.Epidemiol.133(1991)1135-1151Meurmann,O.,etal.:DiagnosisofRespiratorySyncytialVirusinfectioninchildren:comparisonofviralantigendetectionandserology.J.Med.Virol.14(1984)61-65Parrot,R.H.,etal.:RespiratorySyncytialVirusII.Serologicstudiesovera34monthperiodofchildrenwithbronchiolitis,pneumonia,andminorrespiratorydisease.J.Am.Med.Ass.176(1961)653-657Schumacher,R.F.:DieRSV-InfektiondesFrühgeborenen:NosologieundnosokomialeEpidemiologien.Diss.,Freiburg(1990)AdditionalInformation:NameRespiratorysyncytialVirusIgARelatedProductNamesRespiratorysyncytialVirusIgA–ELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.IntendedUseResearchUseOnly

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