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GenWay/Cytomegalovirus (CMV) IgM/GWB-3E8402/1x96 Assays

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GWB-3E8402

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Description:PleaseclickhereforMSDSPDFDatasheetProductName:CytomegalovirusIgMELISA#ofSamples:1x96AssaysIntendedUse:TheCytomegalovirusIgMELISAisintendedforthequalitativedeterminationofIgMclassantibodiesagainstCMVinhumanserumorplasma(citrate).Introduction:Cytomegalovirus(CMV)isamemberoftheherpesvirusgroup(Betasubfamily,DNAvirusof150-200nm).ThesevirusesshareacharacteristicABIlitytoremaindormantwithinthebodyoveralongperiod.InitialCMVinfection,whichmayhavefewsymptoms,isalwaysfollowedbyaprolonged,inapparentinfectionduringwhichthevirusresidesincellswithoutcausingdetectabledamageorclinicalillness.Severeimpairmantofthebody´simmunesystembymedicationordiseaseconsistentlyreactivatesthevirusfromthelatentordormantstate.CMVisfounduniversallythroughoutallgeographiclocationsandsocioeconomicgroups,andinfectsbetween50%and85%ofadults.CMVinfectionismorewidespreadindevelopingcountriesandinareasoflowersocioeconomicconditions.Forthevastmajorityofpeople,CMVinfectionisnotaseriousproblem,butitistocertainhigh-riskgroups:theunbornbabyduringpregnancypeoplewhoworkwithchildrenimmunocompromisedpersons,suchasorgantransplantrecipientsandpersonsinfectedwithHIVThepresenceofvirusresp.infectionmaybeidentifiedbyMicroscopyPCRSEROlogy:CBR,DetectionofantibodiesbyELISAPrinciplesoftheassay:ThequalitativeimmunoenzymaticdeterminationofIgM-classantibodiesagainstCMVisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.MicrotiterstripwellsareprecoatedwithCMVantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgMconjugateisadded.ThisconjugatebindstothecapturedCMV-specificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofCMVspecificIgMantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStability:Thereagentsarestableuptotheexpirydatewhenstoredat2...8°C.LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.Inimmunocompromisedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.ReferencesMaineGT,LazzarottoT,LandiniMP.NewdevelopmentsinthediagnosisofmaternalandcongenitalCMVinfection.ExpertRevMolDiagn.2001May;1(1):19-29.MezzasomaL,Bacarese-HamiltonT,DiCristinaM,RossiR,BistoniF,CrisantiA.Antigenmicroarraysforserodiagnosisofinfectiousdiseases.ClinChem.2002Jan;48(1):121-30.NumazakiK.Cytomegalovirusinfection.NipponRinsho.2001Nov;59Suppl7:112...8.ZaiaJA,SissonsJG,RiddellS,DiamondDJ,WillsMR,CarmichaelAJ,WeekesMP,GandhiM,RosaCL,VillacresM,LaceyS,MarkelS,SunJ.StatusofCytomegalovirusPreventionandTreatmentin2000.Hematology(AmSocHematolEducProgram).2000:339-355.WeberB,BergerA,RabenauH.Humancytomegalovirusinfection:diagnosticpotentialofrecombinantantigensforcytomegalovirusantibodydetection.JVirolMethods.2001Aug;96(2):157-70.AdditionalInformation:NameCytomegalovirus(CMV)IgMRelatedProductNamesCytomegalovirusIgM–ELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatewhenstoredat2...8°C.IntendedUseResearchUseOnly

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