首页 > GenWay > GenWay/TBE / FSME IgG plus quantitative/GWB-3EBB47/1x96 Assays

提醒：代购产品，无质量问题不接受退换货，下单前请仔细核对信息。下单后请及时联系客服核对商品价格，订单生效后再付款。

GenWay/TBE / FSME IgG plus quantitative/GWB-3EBB47/1x96 Assays

价格：

登录查看

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号：

GWB-3EBB47

品牌：

GenWay

会员服务：

尊享会员价

贵宾专线

运费优惠

闪电退款

福利优惠

上门换新

友情提示

以上价格仅为参考，请联系客服询价。

免费咨询热线

4000-520-616

产品说明

Description:PleaseclickhereforMSDSPDFDatasheetProductName:TBE/FSMEIgGplus-ELISA#ofSamples:1x96AssaysIntendedUse:TheTBE/FSMEIgGplus-ELISAisintendedforthequantitativedeterminationofIgGclassantibodiesagainstTBEvirusinhumanserumorplasma(citrate).Introduction:Tick-borneencephalitis(TBE)virusisaflavivirusofthefamilyTogaviridae.Itisanenvelopedsingle-strandedRNAviruswithcubicicosahedralsymmetryandrangesinsizefrom20-80nmindiameter.OntheEuropeancontinentonlytwoantigenicsubtypesexistwhichshowonlylittledifferencesintheirstructuralproteins.TBEvirusismainlytransmittedbyticks.Thedegreeofcontaminationofticks(andthushumans)incentralEuropeincreasesfromwesttoeast,andanybodymaybeaffected.Specificantibodydevelopmentyieldsalife-longimmunity.TBEisthemostimportanttick-transmitteddiseaseofman-besideLymedisease,whichiscausedbythespiRocheteBorreliaburgdorferi.Theclinicalcourseofthediseasedependsontheimmunestatusoftheinfectedpersons.Ahighvirusproductionintheprimaryinfectedtissuesisrequiredforthepassageoftheblood-brainbarrierandtheresultingseveremanifestationsinthecentralnervoussystem.Ofthetwosubtypes,RSSEcausesthemoresevereinfection,havinganincidenceofmortalityofupto25%insomeoutbreaks,whereasmortalityinCEEseldomexceeds5%.AninactivatedTBEvirusvaccineisavailableinEuropeandRussia.Thepresenceofvirusresp.infectionmaybeidentifiedby:Haemagglutination-Inhibition,ComplementFixationDetectionofspecificantibodiesbyCIE,ELISAPrinciplesoftheassay:ThequantitativeimmunoenzymaticdeterminationofIgG-classantibodiesagainstTBEVirusisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.MicrotiterstripwellsareprecoatedwithTBEVirusantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgGconjugateisadded.ThisconjugatebindstothecapturedTBEVirusspecificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofTBEVirusspecificIgGantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.LimitationsoftheTest:Crossreactivitywithantibodiesofotherflaviviruses,forexampleDengueVirus,Yellowfever,Japaneseencephalitisviruses,cannotbeexcluded.Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasSEROlogicaldata.Inimmunocompromizedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.ReferencesRoggendorfM.Frühsommer-MeningoenzephalitisWersollgeimpftwerden?Therapiewoche,40,1173(1990)RoggendorfM.etal.SerologicalDiagnosisofAcuteTick-BorneEncephalitisbyDemonstrationofAntibodiesoftheIgMclass.J.Med.Virol.,7,41(1981)HofmannH.etal.RapiddiagnosisofTick-BorneEncephalitisbyMeansofEnzymeLinkedImmunosorbentAssay.J.Gen.Virol.,42,505(1979)HofmannH.etal.ELISAforIgMAntibodiesagainstTick-BorneEncephalitisVirus:QuantificationandStandardizationofResults.Zbl.Bakt.I.Orig.,255,448(1983)Tick-BorneEncephalitis(TBE)anditsImmunoprophylaxis,IMMUNOAG,Wien(1997)AdditionalInformation:NameTBE/FSMEIgGplusquantitativeRelatedProductNamesTBE/FSMEIgGplus–ELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.IntendedUseResearchUseOnly

GenWay生物科技是一家领先的蛋白质和抗体解决方案供应商。自1998年以来，他们已经提供蛋白质的解决方案和应用研究。迄今为止，GenWay生物科技的在圣地亚哥工厂产生了数以千计的蛋白质和抗体。公司致力于为客户提供研究界最好的试剂。如果产品不符合既定的规格，公司将全额退款。