首页 > GenWay > GenWay/Human RBP (Retinol Binding Protein) ELISA Kit/GWB-418490/96 well

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GenWay/Human RBP (Retinol Binding Protein) ELISA Kit/GWB-418490/96 well

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GWB-418490

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Description:ShortDescription:InthisassaytheRBPpresentinsamplereactswiththeanti-RBPantibodieswhichhavebeenadsorbedtothesurfaceofpolystyrenemicrotitrewells.Aftertheremovalofunboundsampleproteinsbywashing,anti-RBPantibodiesconjugatedwithhorserADIshperoxidase(HRP),areadded.Theseenzyme-labeledantibodiesformcomplexeswiththepreviouslyboundsampleRBP.Followinganotherwashingstep,theenzymeboundtotheimmunosorbentisassayedbytheadditionofachromogenicsubstrate,3,3’,5,5’-tetramethylbenzidine(TMB).ThequantityofboundenzymevariesdirectlywiththeconcentrationofRBPinthesampletested;thus,theabsorbance,at450nm,isameasureoftheconcentrationofRBP.ThequantityofRBPinthetestsamplecanbeinterpolatedfromthestandardcurveconstructedfromthestandards,andcorrectedforsampledilution.AdditionalInformation:NameHumanRBP(RetinolBindingProtein)ELISAKitRelatedProductNamesCRBP;HumanRBP(RetinolBindingProtein)ELISAKit;Cellularretinol-bindingproteinI;CRBP-I;Cellularretinol-bindingprotein;CRBPCRBP1HumanRBPELISA(RetinolBindingProtein)RBPAliasSymbolsPlasmaretinol-bindingprotein,PRBP,PRO2222,RBP,Retinol-bindingprotein4ProteinNameRetinolBindingProteinSwissprotIDP02753SpeciesReactivityHumanAssayInfoAssayMethodology:QuantitativeSandwichELISASensitivity1.163ng/mlKitRange3.125ng/ml-100ng/mlHomologyHumanStandardRangeReferToManualSampleTypeSerum,plasmaandotherBIOLOGicalfluidsProductFormatInSolutionSpecimen/VolumesReferToManualSwissProtNumberP09455ApplicationsELISAReconstitutionandStorage1.DILUENTThe5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.2.WASHSOLUTIONThe20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.3.ENZYME-ANTIBODYCONJUGATEUndilutedhorseradishperoxidaseanti-RBPconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.4.CHROMOGEN-SUBSTRATESOLUTIONTheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.5.STOPSOLUTIONTheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.6.ANTI-HUMANRBPELISAMICROPLATEAnti-HumanRBPcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.7.HUMANRBPCALIBRATORThelyophilizedHumanRBPcalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.ReactivityHumanKitSize96wellKitPrincipleGenWayBiotechRetinolBindingProteinELISAKit(Human)(GWB-418490)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforRetinolBindingProteinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-RetinolBindingProtein-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleRetinolBindingProteincapturedinthewell.KitComponentComponentAmountAnti-HumanRetinolBindingProteinMicroplate96Wells(12x8WellStrips)20XDiluentConcentrate50mL20XWashSolution50mL100XEnzyme-AntibodyConjugate150uLChromogen-SubstrateSolution12mLStopSolution12mLHumanRetinolBindingProteinCalibrator1KitDuration80MinutesStorageRefertokitinstructionsforcomponentstorageLeadTimeDomestic:within1weekdelivery International:1weekIntendedUseResearchUseOnlyAdditionalInformationIntendedUse:TheRetinolBindingProteintestkitisahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringRetinolBindingProteininbiologicalsamplesofHuman.::Reagents:1.DILUENTCONCENTRATE(RunningBuffer)Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.2.WASHSOLUTIONCONCENTRATEOnebottlecontaining50mlofa20Xconcentratedwashsolution.3.ENZYME-ANTIBODYCONJUGATE100XOnevialcontaining150ulofaffinitypurifiedanti-HumanRBPantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer.4.CHROMOGEN-SUBSTRATESOLUTIONOnevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.5.STOPSOLUTIONOnevialcontaining12ml0.3Msulfuricacid.WARNING:Avoidcontactwithskin.6.ANTI-HUMANRBPELISAMICROPLATETwelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-HumanRBP.7.HUMANRBPCALIBRATOROnevialcontainingalyophilizedHumanRBPcalibrator.

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