首页 > GenWay > GenWay/Guinea Pig C3 (Complement Factor 3) ELISA Kit/GWB-4A27B9/96 well

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GenWay/Guinea Pig C3 (Complement Factor 3) ELISA Kit/GWB-4A27B9/96 well

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GWB-4A27B9

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4000-520-616

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Description:ShortDescription:InthisassaytheC3presentinserumsamplereactswiththeanti-C3.antibodieswhichhavebeenadsorbedtothesurfaceofpolystyrenemicrotitrewells.Aftertheremovalofunboundserumproteinsbywashing,anti-C3.antibodiesconjugatedwithhorserADIshperoxidase(HRP),areadded.Theseenzyme-labeledantibodiesformcomplexeswiththepreviouslyboundserumC3.Followinganotherwashingstep,theenzymeboundtotheimmunosorbentisassayedbytheadditionofachromogenicsubstrate,3,3’,5,5’-tetramethylbenzidine(TMB).ThequantityofboundenzymevariesdirectlywiththeconcentrationofC3inthesampletested;thus,theabsorbance,at450nm,isameasureoftheconcentrationofC3inthetestsample.ThequantityofC3inthetestsamplecanbeinterpolatedfromthestandardcurveconstructedfromthestandards,andcorrectedforserumdilution.AdditionalInformation:NameGuineaPigC3(ComplementFactor3)ELISAKitRelatedProductNamesGuineaPigC3(ComplementFactor3)ELISAKit;N/AGuineaPigC3(ComplementFactor3)C3Geneid100135462AliasSymbolsComplementC3,C3,CPAMD1ProteinNameComplementC3DescriptionofTargetC3playsacentralroleintheactivationofthecomplementsystem.ItsprocessingbyC3convertaseisthecentralreactioninbothclassicalandalternativecomplementpathways.AfteractivationC3bcanbindcovalently,viaitsreactivethioester,tocelSwissprotIDP12387SpeciesReactivityGuineaPigAssayInfoAssayMethodology:QuantitativeSandwichELISASensitivity1.399ng/mlKitRange3.125ng/ml-200ng/mlHomologyGuineaPigStandardRangeReferToManualSampleTypeSerum,plasmaandotherBIOLOGicalfluidsProductFormatInSolutionSpecimen/VolumesReferToManualSwissProtNumberP12387ApplicationsELISAReconstitutionandStorage1.DILUENTThe5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.2.WASHSOLUTIONThe20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.3.ENZYME-ANTIBODYCONJUGATEUndilutedhorseradishperoxidaseanti-C3conjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.4.CHROMOGEN-SUBSTRATESOLUTIONTheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.5.STOPSOLUTIONTheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.6.ANTI-GUINEAPIGC3ELISAMICROPLATEAnti-GuineaPigC3coatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.7.GUINEAPIGC3STANDARDSAliquotGuineaPigC3calibratorandstorethemfrozen,avoidrepeatedfreeze-thawcycles.Forstoragelongerthan14days,keepfrozenuntiltheexpirationdate.Storageoflessthan14dayscanbe4C.Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.ReactivityGuineapigKitSize96wellKitPrincipleGenWayBiotechC3ELISAKit(GuineaPig)(GWB-4A27B9)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforComplementC3hasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-ComplementC3-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleComplementC3capturedinthewell.KitComponentComponentAmountAnti-GuineaPigC3Microplate96Wells(12x8WellStrips)20XDiluentConcentrate50mL20XWashSolution50mL100XEnzyme-AntibodyConjugate150uLChromogen-SubstrateSolution12mLStopSolution12mLGuineaPigC3Calibrator1KitDuration25MinutesStorageRefertokitinstructionsforcomponentstorageLeadTimeDomestic:within1weekdelivery International:1weekIntendedUseResearchUseOnlyAdditionalInformationIntendedUse:TheComplementfactor3(C3)testkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringC3inbiologicalsamplesofGuineaPigs.::Reagents:1.DILUENTCONCENTRATE(RunningBuffer)Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.2.WASHSOLUTIONCONCENTRATEOnebottlecontaining50mlofa20Xconcentratedwashsolution.3.ENZYME-ANTIBODYCONJUGATE100XOnevialcontaining150ulofaffinitypurifiedanti-GuineaPigC3antibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer.4.CHROMOGEN-SUBSTRATESOLUTIONOnevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.5.STOPSOLUTIONOnevialcontaining12ml0.3Msulfuricacid.WARNING:Avoidcontactwithskin.6.ANTI-GUINEAPIGC3ELISAMICROPLATETwelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-GuineaPigC3.7.GUINEAPIGC3STANDARDSOnevialcontainingGuineaPigC3calibrator.

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