Description:Short Description: In this assay the HEMOPEXIN present in samples reacts with the anti-HEMOPEXIN antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound HX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HX in the test sample. The quantity of HX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.Additional Information: Name Human Hemopexin ELISA Kit Related Product Names Beta-1B-glycoprotein; Human Hemopexin ELISA Kit; Beta-1B-glycoprotein Human HemopexinHPX Gene Symbol HPX Gene Fullname hemopexin Gene id 3263 Alias Symbols HPX, Hemopexin, Beta-1B-glycoprotein, HPXN Protein Name Hemopexin Description of Target Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Swissprot ID P02790 Species Reactivity Human Assay Info Assay Methodology: Quantitative Sandwich ELISA Sensitivity 2.230 ng/ml Kit Range 6.25 ng/ml - 200 ng/ml Homology Human NCBI Acc Number NP_000604.1 Standard Range Refer To Manual Sample Type Serum, plasma and other biological fluids Product Format In Solution Specimen/Volumes Refer To Manual Swiss Prot Number P02790 Applications ELISA Reconstitution and Storage 1. DILUENTThe 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-HX conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-HUMAN HEMOPEXIN ELISA MICRO PLATEAnti-Human HX coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. HUMAN HEMOPEXIN CALIBRATORThe lyophilized Human Hemopexin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. Reactivity Human Kit Size 96 well Kit Principle Genway Biotech Hemopexin ELISA Kit (Human) (GWB-4B6D1A) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Hemopexin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Hemopexin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hemopexin captured in the well. Kit Component ComponentAmount Anti-Human Hemopexin Microplate96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate50 mL 20X Wash Solution50 mL 100X Enzyme-Antibody Conjugate150 uL Chromogen-Substrate Solution12 mL Stop Solution12 mL Human Hemopexin Calibrator1 Kit Duration 70 Minutes Storage Refer to kit instructions for component storage Lead Time Domestic: within 1 week delivery International: 1 week Intended Use Research Use Only Additional Information Intended Use: The Hemopexin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemopexin in serum, plasma of Human. :: Reagents: 1. DILUENT CONCENTRATE (Running Buffer)One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100XOne vial containing 150 ul of affinity purified anti-Human Hemopexin antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTIONOne vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTIONOne vial containing 12 ml 0.3 M sulfuric acid.WARNING: Avoid contact with skin.6. ANTI-HUMAN HEMOPEXIN ELISA MICRO PLATETwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human HX.7. HUMAN HEMOPEXIN CALIBRATOROne vial containing a lyophilized Human Hemopexin calibrator.