Description:Short Description: In this assay the IgE present in samples reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.Additional Information: Name Rat IgE ELISA Kit Related Product Names Rat IgE ELISA Kit; N/A Rat IgEIGHE Alias Symbols IGHE, Ig epsilon chain C region, IgE Protein Name Ig epsilon chain C region Swissprot ID P01855 Species Reactivity Rat Assay Info Assay Methodology: Quantitative Sandwich ELISA Sensitivity 0.213 ng/ml Kit Range 1 ng/ml - 32 ng/ml Homology Rat Standard Range Refer To Manual Sample Type Serum, plasma and other biological fluids Product Format In Solution Specimen/Volumes Refer To Manual Swiss Prot Number P01854 Applications ELISA Reconstitution and Storage 1. DILUENTThe 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-IgE conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-RAT IgE ELISA MICRO PLATEAnti-Rat IgE coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. RAT IgE CALIBRATORLong Term Storage: upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. Reactivity Rat Kit Size 96 well Kit Principle Genway Biotech IgE ELISA Kit (Rat) (GWB-500760) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Ig epsilon chain C region has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Ig epsilon chain C region - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ig epsilon chain C region captured in the well. Kit Component ComponentAmount Anti-Rat IgE Microplate96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate50 mL 20X Wash Solution50 mL 100X Enzyme-Antibody Conjugate150 uL Chromogen-Substrate Solution12 mL Stop Solution12 mL Rat IgE Calibrator1 Kit Duration 130 Minutes Storage Refer to kit instructions for component storage Lead Time Domestic: within 1 week delivery International: 1 week Intended Use Research Use Only Additional Information Intended Use: The IgE test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgE in biological samples of rats. :: Reagents: 1. DILUENT CONCENTRATE (Running Buffer)One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100XOne vial containing 150 ul of affinity purified anti-Rat IgE antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTIONOne vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTIONOne vial containing 12 ml 0.3 M sulfuric acid.WARNING: Avoid contact with skin.6. ANTI-RAT IgE ELISA MICRO PLATETwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat IgE.7. RAT IgE CALIBRATOROne vial containing Rat IgE calibrator.