首页 > GenWay > GenWay/Helicobacter Pylori IgG Enzyme Immunoassay Test Kit/GWB-504F20/ELISA_Kits

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GenWay/Helicobacter Pylori IgG Enzyme Immunoassay Test Kit/GWB-504F20/ELISA_Kits

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GWB-504F20

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Description:Species:HumanSampleType:SerumForthequantitativedeterminationofIgGAntibodiestoHelicobacterpyloriinserum.BacterialInfections,Infection,Blood,GastrointestinalDiseases,GastrointestinalTractProductName:IgGAntibodiestoHelicobacterpyloriSampleType:Serum#ofSamples:1X96AssaysIntendedUse:TheHelicobacterpyloriIgGTestKitisintendedforuseinevaluatingtheSEROlogicstatustoH.pyloriinfectioninspecimenswithgastrointestinalsymptoms.Introduction:HelicobacterpyloriisaspiralbacteriumculturedfromhumangastricmucosadiscoveredbyB.J.Marshallin1982.StudieshaveindicatedthatthepresenceofH.pyloriisassociatedwithavarietyofgastrointestinaldiseasesincludinggastritis,duodenalandgastriculcers,non-ulcerdyspepsia,andgastricadenocarcinomaandlymphoma.Thestudieshavealsodemonstratedthatremovaloftheorganismbyanti-microbialtherapyiscorrelatedwiththeresolutionofsymptomsandcureofdiseasesPrincipleofthetest:PurifiedH.pyloriantigeniscoatedonthesurfaceofmicrowells.Dilutedsamplesserumisaddedtothewells,andtheH.pyloriIgG-specificantibody,ifpresent,bindstotheantigen.Allunboundmaterialsarewashedaway.Enzymeconjugateisadded,whichbindstotheantibody-antigencomplex.Excessenzymeconjugateiswashedoffandsubstrateandchromogenareadded.Theenzymeconjugatecatalyticreactionisstoppedataspecifictime.TheintensityofthecolorgeneratedisproportionaltotheamountofIgG-specificantibodyinthesample.Theresultsarereadbyamicrowellreadercomparedinaparallelmannerwithcalibratorandcontrols.StorageandStABIlity:2-8CLimitationoftheTest:1.ReliableandreproducIBLeresultswillbeobtainedwhentheassayprocedureiscarriedoutwithacompleteunderstandingofthepackageinsertinstructionsandwithadherencetogoodlaboratorypractice.2.Thewashprocedureiscritical.InsufficientwashingwillresultinpoorprecisionandfalselyelevatedabsorbancereADIngs.3.Serumsamplesdemonstratinggrosslipemia,grosshemolysis,orturbidityshouldnotbeusedwiththistest.Precision:AstudywasperformedtodocumenttypicalassayprecisionwiththeGenWayBiotechH.pyloriIgGEIAproduct.Themean,SD,and%CVwerecalculatedforIntra-andInter-AssayinO.D.value(A450).ProductName:IgGAntibodiestoHelicobacterpyloriSubcellularLocation:Secreted.Disease:ChromosomalaberrationsinvolvingIGHG1maybeacauseofmultiplemyeloma[MIM:254500].Translocationt(11;14)(q13;q32)withCCND1;translocationt(4;14)(p16.3;q32.3)withFGFR3;translocationt(6;14)(p25;q32)withIRF4.Miscellaneous:NiehastheG1M(17)allotypicMarker,97-K,andtheG1M(1)markers,239-Dand241-L.KOLandEUsequenceshavetheG1M(3)markerandtheG1M(non-1)markers.Miscellaneous:Niealsodiffersintheamidationstatesof35,116,198,269and272.Miscellaneous:EUalsodiffersintheamidationstatesofresidues155,166,177,195,198,269,and272andintheorderofresidues268-272.Miscellaneous:KOLalsodiffersintheamidationstatesofresidues198,267and272.AdditionalInformation:NameHelicobacterPyloriIgGEnzymeImmunoassayTestKitRelatedProductNamesIgGAntibodiestoHelicobacterpyloriELISAKit;N/AIgGAntibodiestoHelicobacterpyloriIGHG1MolecularWeight36106SwissProtNumberP01857IntendedUseResearchUseOnly

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