首页 > GenWay > GenWay/beta-HCG/GWB-519AAA/1x96 Assays

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GenWay/beta-HCG/GWB-519AAA/1x96 Assays

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GWB-519AAA

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GenWay

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4000-520-616

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Description:ProductName:b-HCG#ofSamples:1x96AssaysIntendedUse:Immunoenzymaticcolorimetricmethod(ELISA)forquantitativedeterminationofb-HCGinserumorplasma.Introduction:Humanchorionicgonadotropin(hCG)isaglycoproteinhormonesecretedinpregnancy,thatismadebytheembryosoonafterconceptionandlaterbythesyncytiotrophoblast(partoftheplacenta).Itsroleistopreventthedisintegrationofthecorpusluteumoftheovaryandtherebymaintainprogesteroneproductionthatiscriticalforapregnancyinhumans.hCGmayhaveadditionalfunctions,forinstanceitisthoughtthatitaffectstheimmunetoleranceofthepregnancy.PregnancytestsmeasurethelevelsofhCGinthebloodorurinetoindicatethepresenceorabsenceofanimplantedembryo.Inparticular,pregnancytestsemployanantibodythatisspecifictotheβ-subunitofhCG(βhCG).ThisisimportantsothattestsdonotmakefalsepositivesbyconfusinghCGwithLHandFSH.βhCGisalsosecretedbysomecancersincludingteratomas,choriocarcinomas.But,elevatedlevelscannotprovethepresenceofatumor,andlowlevelsdonotruleitout.Principlesoftheassay:Theb-HCGassayisbasedonsimultaneousbindingofhumanb-HCGtotwomonoclonalantibodies;oneisimmobilizedonthemicroplate,theotherissolubleandconjugatedwithhorserADIshperoxidase(HRP).Microtiterstripwellsareprecoatedwithanti-b-HCGIgGantibodies.b-HCGinsamplesandstandardsbindstotheimmobilisedantibodiesonthesurfaceofthemicrotiterwellsandthesecond,solubleanti-b-HCGantibody-enzymeconjugatebindstotheimmobileantibody-b-HCG-complexduringthefirstincubation.Afterwardsawashingstepisperformed.TheimmunecomplexisvisualizedbyaddingTetramethylbenzidine(TMB)substrate,whichgivesabluereactionproduct.Theintensityofthisproductisproportionaltotheamountofb-HCGinsamplesandstandards.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorptionat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlity:Theclosedreagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.ReferencesWisdom,G.B.,Clin.Chem.(1976)22/8,12431255Shome,B.andParlow,A.F.;J.Clin.Endocr.Metab.(1974)39,199202Uotila,M.,Ruoslahti,E.,Engvall,E.J.,J.Immunol.Methds(1981)42,1115Acosta,A.A.M.D.andWright,G.L.,JournalofClinicalImmunoassays(1983)6,41Cohen,K.L.,Metabolism(1977)26,11651177Lindstedt(1982)Scand.J.Clin.Lab.Invest42,201Chien(1987)J.Clin.Endocr.Metab.64,313AdditionalInformation:Namebeta-HCGRelatedProductNamesb-HCGMolecularWeight0.5StorageTheclosedreagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.IntendedUseResearchUseOnly

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