首页 > GenWay > GenWay/Human Alpha 1-Antitrypsin ELISA Kit/GWB-5428A0/96 well

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GenWay/Human Alpha 1-Antitrypsin ELISA Kit/GWB-5428A0/96 well

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GWB-5428A0

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AdditionalInformation:NameHumanAlpha1-AntitrypsinELISAKitRelatedProductNamesAlpha-1proteaseinhibitor,Alpha-1-antiproteinase,Humanalpha1-AntitrypsinELISAKit;Alpha-1proteaseinhibitor,Alpha-1-antiproteinase,AAT,PIHumanalpha1Antitrypsin,SERPINA1,PI,AATELISA,A1AT,HumanAlpha1-AntitrypsinELISAKitGeneSymbolSERPINA1GeneFullnameserpinfamilyAmember1Geneid5265AliasSymbolsSERPINA1,AAT,A1AT,alpha1ATProteinNameAlpha-1-antitrypsinDescriptionofTargetInhibitorofserineproteases.Itsprimarytargetiselastase,butitalsohasamoderateaffinityforplasminandthrombin.IrreversIBLyinhibitstrypsin,chymotrypsinandplasminogenactivator.Theaberrantforminhibitsinsulin-inducedNOsynthesisinSwissprotIDP01009SpeciesReactivityHumanAssayInfoAssayMethodology:QuantitativeSandwichELISASensitivity1.094ng/mlKitRange7.81ng/ml-500ng/mlHomologyHumanStandardRangeReferToManualSampleTypeSerum,plasmaandotherBIOLOGicalfluidsProductFormatInSolutionSpecimen/VolumesReferToManualApplicationsELISAReconstitutionandStorage1.DILUENTThe5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.2.WASHSOLUTIONThe20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.3.ENZYME-ANTIBODYCONJUGATEUndilutedhorserADIshperoxidaseanti-A1ATconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.4.CHROMOGEN-SUBSTRATESOLUTIONTheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.5.STOPSOLUTIONTheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.6.ANTI-HUMANALPHA1-ANTITRYPSINELISAMICROPLATEAnti-HumanA1ATcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.7.HUMANALPHA1-ANTITRYPSINCALIBRATORThelyophilizedHumanAlpha1-Antitrypsincalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.ReactivityHumanKitSize96wellKitPrincipleGenWayBiotechAlpha1-AntitrypsinELISAKit(Human)(GWB-5428A0)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforAlpha-1-antitrypsinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Alpha-1-antitrypsin-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleAlpha-1-antitrypsincapturedinthewell.KitComponentComponentAmountAnti-HumanAlpha1-AntitrypsinMicroplate96Wells(12x8WellStrips)20XDiluentConcentrate50mL20XWashSolution50mL100XEnzyme-AntibodyConjugate150uLChromogen-SubstrateSolution12mLStopSolution12mLHumanAlpha1-AntitrypsinCalibrator1KitDuration100MinutesStorageRefertokitinstructionsforcomponentstorageLeadTimeDomestic:within1weekdelivery International:1weekIntendedUseResearchUseOnlyAdditionalInformationIntendedUse:TheAlpha1-Antitrypsin(A1AT)testkitisahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringA1ATinbiologicalfluidofhumans.::Reagents:1.DILUENTCONCENTRATE(RunningBuffer)Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.2.WASHSOLUTIONCONCENTRATEOnebottlecontaining50mlofa20Xconcentratedwashsolution.3.ENZYME-ANTIBODYCONJUGATE100XOnevialcontaining150ulofaffinitypurifiedanti-HumanAlpha1-AntitrypsinantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer.4.CHROMOGEN-SUBSTRATESOLUTIONOnevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.5.STOPSOLUTIONOnevialcontaining12ml0.3Msulfuricacid.WARNING:Avoidcontactwithskin.6.ANTI-HUMANALPHA1-ANTITRYPSINELISAMICROPLATETwelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-HumanA1AT.7.HUMANALPHA1-ANTITRYPSINCALIBRATOROnevialcontainingalyophilizedHumanAlpha1-Antitrypsincalibrator.

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