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GenWay/Echinococcus IgG (Echinococcosis, Hydatidosis)/GWB-5571AC/1x96 Assays

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GWB-5571AC

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Description:PleaseclickhereforMSDSPDFDatasheetProductName:EchinococcusIgG-ELISA#ofSamples:1x96AssaysIntendedUse:TheEchinococcusIgG-ELISAisintendedforthequalitativedeterminationofIgGclassantibodiesagainstEchinococcusinhumanserumorplasma(citrate).Introduction:Echinococciaremicroscopiccestodes(tapeworms)of1-6mmwhicharedependentontheirgenusfoundeitherindogsorothercanids(E.granulosus)orinfoxes,coyotesandwolves(E.multilocularis).Intheirlarvalstagetheyarethecausativeagentofhumanechinococcosis(Hydatiosis,orhydaticdisease).Theadulttapewormsresideinthesmallbowelofthedefinitivehosts,andgravidproglottidsreleaseeggsthatarepassedinthefeces.Afteringestionofasuitableintermediatehost,theegghatchesinthesmallbowelandreleasesanoncospherethatpenetratestheintestinalwallandthroughthecirculatorysystemintovariousorgans,especiallytheliverandlungs,whereitdevelopsintoacyst.Echinococcusinfectionsremainsilentforyearsbeforetheenlargingcystscausesymptomsintheaffectedorgans(liver,lung,andlesscommonlyotherorgansasbrain,bone,heart).E.granulosusoccurspracticallyworldwide,andmorefrequentlyinrural,grazingareaswheredogsingestorgansfrominfectedanimals.E.multilocularisoccursinthenorthernhemisphere,includingcentralEuropeandthenorthernpartsofEurope,Asia,andNorthAmerica.Althoughhumancasesarerare,infectioninhumanscausesparasitictumorstoformintheliver,thelungs,andlesscommonly,thebrain,andotherorgans.Ifleftuntreated,infectioncanbefatal.InfectionmaybeidentifiedbyMicroscopySEROlogy:IHA,IFA,DetectionofantibodiesbyELISADetectableimmuneresponseshavebeenassociatedwiththelocation,integrity,andvitalityofthelarvalcyst.Cystsintheliveraremorelikelytoelicitantibodyresponsethancystsinthelungs,andregardlessoflocalization,antibodydetectiontestsareleastsensitiveinpatientswithintacthyalinecysts.Cystsinthelungs,brain,andspleenareassociatedwithloweredserodiagnosticreactivitywhereasthoseinboneappeartomoreregularlystimulatedetectableantibody.Fissurationorruptureofacystisfollowedbyanabruptstimulationofantibodies.Principlesoftheassay:ThequalitativeimmunoenzymaticdeterminationofIgG-classantibodiesagainstEchinococcusisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.MicrotiterstripwellsareprecoatedwithEchinococcusantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgGconjugateisadded.ThisconjugatebindstothecapturedEchinococcus-specificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine-(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofEchinococcus-specificIgGantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.CrossreactionswithTaeniasoliuminfectionscanoccur.IfTaeniasoliumcannotberuledoutinthedifferentialdiagnosis,apositivesampleshouldbeconfirmedbyanothermethod.Inimmunocompromisedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.ReferencesEchinococcus,Erkennung,VerhütungundBekämpfung,Bundesgesundheitsblatt1997;40:MikroBIOLOGie7,182-184,1997P.M.Schantz,D.Shanksetal.AmJ.Trop.Med.Hyg.29(4);609-612,1980Al.Yaman,F.M.,andKnobioch.1989.Isolationandparticharacterizationofspecies-specificandcross-reactiveantigenofEchinococcusgranulosuscystfluid.Mol.Biochem.Parastol.37:101-107Gottstein,B.1985.PurificationandcharacterizationofaspecificantigenfromEchinococcusmultilocularis.ParasiteImmuno.7:201-212Gottstein,B.1992.Molecularandimmunologicaldiagnosisechinococcusis,Clin.Microbiol.Rev.5:248-261AdditionalInformation:NameEchinococcusIgG(Echinococcosis,Hydatidosis)RelatedProductNamesEchinococcusIgG-ELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.IntendedUseResearchUseOnly

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