Description:1.IntendedUse TheGenWayBrucellaIgGAntibodyELISATestKithasbeendesignedforthethedetectionand thequantitativedeterminationofspecificIgGantibodiesagainstBrucellainserumandplasma. FurtherapplicationsinotherbodyfluidsarepossIBLeandcanberequestedfromtheTechnical ServiceofGenWay. Thisassayisintendedforresearchuseonly. 2.GeneralInformation Brucellosisisaninfectiousdiseasecausedbysmallellipsoid,gram-negativebacteria.Thereare fourdifferentgerms: Br.abortus, Br.melitensis, Br.suisand Br.canis.Peopleareinfectedby contactwithinfectedanimalsorbyeatingmeatordrinkingunpasteurizedmilkfrominfected animals.Asarule,infectedhumansarenotcontagious.Brucellosisismostfrequentinyoungand middle-agedindividuals.Endangeredpersonsarebutchers,farmers,ownersofpets,veterinaries andtouristsinSoutherncountries.Theappearanceofbrucellosisshowsaprevalenceduringwinter andspring. Theincubationperiodisbetweenoneandthree weeks,butmaybeaslongastwomonths.Br. abortusand Br.melitensismaycauseBang’sDisease,orinrarecasesMaltaFever.Thefirst appearsoccasionallywithalowpathogenicityforman.TypicalsymptomsforBang’sDiseaseare periodicallyoccurringfever,splenomegalyand swellingoflymphnodes.Insomecasesan inflammationofdifferentjointsandorgansoccurs.TheMaltaFeveriscausedbytheepidemictype ofbrucellosis,andinfectionalmostalwaysleadstoamanifestillness.Someinfectionswith BrucellacancauseBrucellaHepatitis.Itispossiblethatthereisalinkbetweenaninfectionwith Brucellaandtheoutbreakofmultiplesclerosis. Duringanantibiotictherapyorachronicinfection,thedetectionofBrucellaspec.inblood,urine, cerebrospinalfluid,sputumorotherbodyfluidscouldbenegative.SEROlogicalmethodslike agglutination,complementfixationreaction, BrucellaCoombstestandELISAaregood alternatives.Themonitoringofantibodiescanserveasausualindicationofthestatusofinfection. Duringthefirstdays,IgMistheonlyimmunoglobulinthatappears.Asthediseaseprogresses,IgM recedesquantitativelyandIgGbecomespredominant.Inchronicbrucellosis,IgGmaybeproduced foranextendedperiod. 3.PrincipleoftheTest TheGenWayBrucellaIgGantibodytestkitisbasedontheprincipleoftheenzymeimmunoassay (EIA).Brucellaantigenisboundonthesurfaceofthemicrotiterstrips.Dilutedsampleserumor ready-to-usestandardsarePipettedintothewellsofthemicrotiterplate.AbindingbetweentheIgG antibodiesoftheserumandtheimmobilizedBrucellaantigentakesplace.Afteraonehour incubationatroomtemperature,theplateisrinsedwithdilutedwashsolution,inordertoremove unboundmaterial.Thenready-to-useanti-human-IgGperoxidaseconjugateisaddedandincubated for30minutes.Afterafurtherwashingstep,thesubstrate(TMB)solutionispipettedandincubated for20minutes,inducingthedevelopmentofabluedyeinthewells.Thecolordevelopmentis terminatedbytheadditionofastopsolution,whichchangesthecolorfrombluetoyellow.The resultingdyeismeasuredspectrophotometricallyatthewavelengthof450nm.Theconcentration oftheIgGantibodiesisdirectlyproportionaltotheintensityofthecolor. AdditionalInformation:NameBrucellaIgGELISARelatedProductNamesBrucellaIgGELISAApplicationsEnzymeimmunoassaybasedonmicrotiterplateforthedetectionandquantitativedeterminationofhumanIgGantibodiesagainstBrucellainserumandplasmaIntendedUseResearchUseOnly