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GenWay/Epstein Barr Virus (EBNA) IgG/GWB-5ED61E/1x96 Assays

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GWB-5ED61E

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Description:PleaseclickhereforMSDSPDFDatasheetProductName:Epstein-BarrVirus(EBNA)recombinantIgGELISA#ofSamples:1x96AssaysIntendedUse:TheEpstein-BarrVirus(EBV)IgG-ELISAisintendedforthequalitativedeterminationofIgGclassantibodiesagainstEpstein-Barrvirus(EBNA)inhumanserumorplasma(citrate).Introduction:Epstein-BarrVirus(EBV)isamemberoftheherpesvirusfamily(Gammasubgroup,DNAvirusof120-200nm)andoneofthemostcommonhumanviruses.Thevirusoccursworldwide,andmostpeoplebecomeinfectedwithEBVsometimeduringtheirlives.TransmissionofthevirusisalmostimpossIBLetopreventsincemanyhealthypeoplecancarryandspreadthevirusintermittentlyforlife.InfantsbecomesusceptibletoEBVassoonasmaternalantibodyprotectiondisappears.Infectionofchildrenusuallycausesnosymptoms.Infectionduringadolescenceoryoungadulthoodcausesinfectiousmononucleosis35%to50%ofthetime.Infectiousmononucleosisisalmostneverfatal.TherearenoknownassociationsbetweenactiveEBVinfectionandproblemsduringpregnancy,suchasmiscarriagesorbirthdefects.Althoughthesymptomsofinfectiousmononucleosisusuallyresolvein1or2months,EBVremainsdormantorlatentinafewcellsinthethroatandbloodfortherestofthepersonslife.Periodically,theviruscanreactivateandiscommonlyfoundinthesalivaofinfectedpersons.Thisreactivationusuallyoccurswithoutsymptomsofillness.EBValsoestablishesalifelongdormantinfectioninsomecellsofthebodysimmunesystem.AlateeventinaveryfewcarriersofthisvirusistheemergenceofBurkitt´slymphomaandnasopharyngealcarcinoma,butEBVisprobablynotthesolecauseofthesemalignancies.Thepresenceofvirusresp.infectionmaybeidentifiedbyPCRSEROlogy:monospottest,DetectionofantibodiesbyELISATheoptimalcombinationofserologictestingconsistsofthetitrationoffourMarkers:IgMandIgGtotheviralcapsidantigen(VCA),IgMtotheearlyantigen,andantibodytoEBVnuclearantigen(EBNA).IgMtoVCAappearsearlyininfectionanddisappearswithin4to12weeks.IgGtoVCAappearsintheacutephase,peaksat2to4weeksafteronset,declinesslightly,andthenpersistsforlife.Ifantibodiestotheviralcapsidantigenarenotdetected,thepatientissusceptibletoEBVinfection.Principlesoftheassay:ThequalitativeimmunoenzymaticdeterminationofIgG-classantibodiesagainstEpstein-BarrVirusisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.MicrotiterstripwellsareprecoatedwithrecombinantEpstein-BarrVirusnulcearantigenEBNA-1tobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgGconjugateisadded.ThisconjugatebindstothecapturedEpstein-BarrVirus-specificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine-(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofEpstein-BarrVirus-specificIgGantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.LimitationsoftheTest:Anegativeresult(IgGorIgM)cannotexcludeaninfectionwithEpsteinBarrVirus.Especiallyintheearlystateofinfectionitispossiblethattheamountofantibodiesisbelowthedetectionlimit.IncaseofclinicalsUSPiciontoEBVorequivocaltestresultitisrecommendedtotestanothersampleafter2-3weeks.Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.Inimmunocompromisedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.ReferencesBergmanM.,GleckmanR.A.,Heterophil-negativeinfectiousmononucleosis-likesyndrome.Postgrad.Med.,81(1):313-326(1987)BuchwaldD.,KomaroffA.L.,Reviewoflaboratoryfindingsforpatientwithchronicfatiquesyndrome.Rev.Inf.Dis.,13(Suppl.1):S12-S18(1991)DeOryF.,AntonayaJ.,FernandezM.V.,EchevarriaJ.M.,Applicationoflow-AvidityimmunglobulinGstudiestodiagnosisofEBVinfectiousmononucleosis.J.Clin.Microbiol.,31(6):1669-1671,(1993)De-The,G.,EpidemiologyofEBVandAssociatedDiseasesinMan,In:TheHerpesviruses,Roizman,B.(ed).,Volume1,NewYork:Plenum-Press,25-103,(1982)DölkenG.,WeitzmannU.,BoldtC.etal..EnzymelinkedimmunosorbentassayforIgGantibodiesofEBVvirusassociatedearlyantigensandviralcapsideantigen.J.Immunol.Meth..,67:225-233,(1984)FärberI.,WutzlerP.,WohlrabeP.etal.,Serologicaldiagnosisofinfectiousmononucleosisusingthreeanti-Epstein-BarrvirusrecombinantELISAs,J.Virol.Meth.;42:301-308,(1993)Gorgievski-HrisohoM.,HindererW.,NEBel-SchickelH.etal.,SerodiagnosisofinfectiousmononucleosisbyusingrecombinantEpstein-Barrvirusantigensandenzyme-linkedImmunosorbentassaytechnology,J.Clin.Microbiol.,28(10):2305-2311,(1990)HalprinJ.,ScottA.L.,JacobosonL.etal.,Enzyme-linkedimmunosorbentassayofantibodiestoEpstein-Barrvirusnuclearandearlyantigensinpatientswithinfectiousmononucleosisandnasopharyngealcarcinoma,Ann.Int.Med.,104:331-337,(1986).Heath,C.W.,A.L.Brodsky,andA.L.Ptolosky,InfectiousMononuclelosisinageneralpopulation,Am.J.Epiemiology,95(1):46-52,(1972)HenleW.,HenleGE.,HorwitzCA.,Epstein-Barrvirusspecificgiagnostictestsininfectiousmononucleosis,HumanPathol.,5(5):551-565,(1974)LamyME.,FavartAM.,CornuA.etal.,StudyofEpstein-Barrvirus(EBV)antibodies:IgGandIgManti-VCA,IgGantiEAandIganti-EBNAobtainedwithanoriginalmicrotitertechnique.SerologicalcriterionsofprimaryandrecurrentsEBVinfectionsandfollow-upofinfectiousmononucleosis.SeroepidemiologyofEBVinBelgiumbasedon5178serafrompatients.ActaClin.Belg.,37(5):281-298,(1982)LennetteE.,Epstein-BarrVirus,inManualofClinicalMicroBIOLOGy,4thed.WashingtonD.C.,Am.Soc.Microbiol.P728-732,(1985)LukaJ.,chasePC.,PearsonGR.,Asensitiveenzyme-linkedImmunosorbentassay(ELISA)againstthemajorEBV-associatedantigens.I-CorrelationbetweenELISAandimmunofluorescencetitersusingpurifiedantigens,J.Immunol.Metho.,67:145-156,(1984)PearsonGP.,InfectiousMononucleosis:thehumoralresponse,In:Infectiousmononucleosis,D.Schlossberged.,Springer-Verlag,NewYork,p.89-99,(1989)PocheldyC.,Laboratorytestingforinfectiousmononucleosis:cautionstoobserveininterpretingresults.Prostgrad.Med.,81(1):335-342(1987)PurtiloBT.,HinrichsS.,DetectionofEpstein-BarrVirusinduceddeseasesbylaboratorytechniques,InCSTarMonograph,(1993)AdditionalInformation:NameEpsteinBarrVirus(EBNA)IgGRelatedProductNamesEpstein-BarrVirus(EBNA)recombinantIgG–ELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.IntendedUseResearchUseOnly

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