首页 > GenWay > GenWay/FREE TESTOSTERONE/GWB-60224F/1x96 Assays

提醒：代购产品，无质量问题不接受退换货，下单前请仔细核对信息。下单后请及时联系客服核对商品价格，订单生效后再付款。

GenWay/FREE TESTOSTERONE/GWB-60224F/1x96 Assays

价格：

登录查看

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号：

GWB-60224F

品牌：

GenWay

会员服务：

尊享会员价

贵宾专线

运费优惠

闪电退款

福利优惠

上门换新

友情提示

以上价格仅为参考，请联系客服询价。

免费咨询热线

4000-520-616

产品说明

Description:ProductName:FreeTestosterone#ofSamples:1x96AssaysIntendedUse:CompetitiveimmunoenzymaticcolorimetricmethodforquantitativedeterminationoffreeTestosteroneinserumorplasma.Introduction:Testosteroneisasteroidhormonefromtheandrogengroup.Testosteroneisprimarilysecretedinthetestesofmalesandtheovariesoffemalesalthoughsmallamountsaresecretedbytheadrenalglands.Itistheprincipalmalesexhormoneandananabolicsteroid.Inbothmalesandfemales,itplayskeyrolesinhealthandwell-being.Only1-2%ofcirculatingtestosteroneexistsasunboundorfreetestosterone.Themajority,approximately60%,isboundtoSHBGwithhighaffinity,whiletheremainderislooselyboundtoalbumin.Boththealbumin-boundandfreefractionsmaybeBIOLOGicallyactive,whileSHBGeffectivelyinhibitstestosteroneaction.Testosteroneeffectscanbeclassifiedasvirilisingandanaboliceffects.Anaboliceffectsincludegrowthofmusclemassandstrength,increasedbonedensityandstrength,andstimulationoflineargrowthandbonematuration.Virilisingeffectsincludematurationofthesexorgans.Testosteronelevelsdeclinegraduallywithageinmen.Measurementofthefreeorunboundfractionofserumtestosteronehasbeenproposedasameansofestimatingthephysiologicallybioactivehormone.Freetestosteronelevelsareelevatedinwomenwithhyperandrogenismassociatedwithhirsutisminthepresenceorabsenceofpolycysticovariandisease.Inaddition,freetestosteronemeasurementsmaybemoreusefulthantotaltestosteroneinsituationswhereSHBGisincreasedordecreased(e.g.hypothyroidismandobesity).Principlesoftheassay:Microtiterstripwellsareprecoatedwithanti-Testosteroneantibodies(solid-phase).TestosteroneinthesamplecompeteswithaddedhorserADIshperoxidaselabelledTestosterone(enzyme-labelledantigen)forantibodybinding.Afterincubationabound/freeseparationisperformedbysolid-phasewashing.Theimmunecomplexformedbyenzyme-labelledantigenisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisinverselyproportionaltotheamountofTestosteroneinthesample.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorptionat450nmisreadusinganELISAmicrowellplatereader.TestosteroneinthebloodisboundtoSHBG(60%)andinlowerquantitytootherprotein.OnlythemeasurementofFreeTestosterone(< 1% of Total Testosterone) permits the estimating of the hormone biologically active.StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°Cinthedark.LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.ReferencesMcCannD.,KirkishL.(1985)J.Clin.Immunoassay8,234-6.EkinsRP.(1984)J.Clin.Immunoassay7(2),16380.PaulsonJDetal.(1977)Am.JObst.Gynecol128,8517.OdlindV.etal.(1982)Clin.Endocrinology16,24349.GreenPJ.(1982)ClinChem28,1237.WuCH.(1982)ObstetGynecol.60,18894.AdditionalInformation:NameFREETESTOSTERONERelatedProductNamesFreeTestosteroneMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°Cinthedark.IntendedUseResearchUseOnly

GenWay生物科技是一家领先的蛋白质和抗体解决方案供应商。自1998年以来，他们已经提供蛋白质的解决方案和应用研究。迄今为止，GenWay生物科技的在圣地亚哥工厂产生了数以千计的蛋白质和抗体。公司致力于为客户提供研究界最好的试剂。如果产品不符合既定的规格，公司将全额退款。