首页 > GenWay > GenWay/Herpes simplex Virus 2 (HSV 2) IgG recombinant antigens/GWB-6271A3/1x96 Assays

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GenWay/Herpes simplex Virus 2 (HSV 2) IgG recombinant antigens/GWB-6271A3/1x96 Assays

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GWB-6271A3

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Description:ProductNameHerpesSimplexVirus2(HSV2)IgG-ELISA.#ofSamples1x96Assays.IntendedUseTheHSV2IgGrecombinantELISAallowsthedetectionofHSV2infectioninpresenceofantibodiestoHSV1inhumanserumorplasma(citrate).IntroductionHerpessimplexisanenvelopedDNAvirus(150-200nmindiameter)belongingtothealpha-herpesviridae.Basedonantigenic,biochemicalandBIOLOGicallydifferencesitcanbedividedintotwoSEROtypes,HSV-1andHSV-2.Manistheonlyknownnaturalhostandsourceofthevirus.HSVtype1typicallycausesoralherpes,whileHSVtype2typicallyaffectsthegenitalarea.Mostofthetime,HSV-1andHSV-2areinactive,orsilent,andcausenosymptoms,butsomeinfectedpeoplehaveoutbreaksofblistersandulcers.OnceinfectedwithHSV,peopleremaininfectedforlife.Herpessimplexvirusesareamongstthemostcommoninfectiousagentsofman,andeitherHSVtypeappearstobecapableofinfectingsimilarbodysites.Ahighpercentageoftheadultpopulationisseropositive(appr.90%HSV-1,independenceonthesocio-economicstatus10-30%HSV-2).PrimaryHSV-1infectionusuallyoccursinearlychildhood(6to18monthsofage).HSV-2usuallyproducesmildsymptoms,andmostpeoplehavenorecognizedsymptoms.PersonsatriskarechildrenwithinheritedT-celldeficienciesandpatientswhoareimmunosuppressedbecauseofinfection(e.g.HIV),transplantation,orcancertherapy.HSV-2cancausepotentiallyfatalinfectionsininfantsifthemotherissheddingvirusatthetimeofdelivery.-HSV-2mayplayamajorroleintheheterosexualspreadofHIV:herpescanmakepeoplemoresusceptIBLetoHIVinfection,andHIV-infectedindividualsmoreinfectious.Thepresenceofvirusresp.infectionmaybeidentifiedbyMicroscopy:CPE,IFPCRSerology:DetectionofantibodiesbyELISA.PrinciplesoftheassayRecombinantgG2proteinofHSV-2showdiffusebandinSDSelectrophoresiscorrespondingtogG2whichisfoundatapproximately43kD.ThisantigenwillallowthedetectionofHSV2infectioninthepresenceofantibodiestoHSV1.Thisisimportantasthe%ofthepopulationwithHSV1increasesthroughoutlifeupto>90%atoldage.HSV2isgenerallysexuallytransmittedandinallsocietiesisassociatedwiththeamountofsexualactivityandnumberofpartners.Genitalherpesisimportanttodiagnoseinpregnancyassheedingviruscanleadtocongenitalinfectionofthechildwitchisinvariablyserious.ThequalitativeimmunoenzymaticdeterminationofIgG-classantibodiesagainstHSVType2isbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.MicrotiterstripwellsareprecoatedwithHSVType2recombinantantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgGconjugateisadded.ThisconjugatebindstothecapturedHSVType2specificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofHSVType2specificIgGantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlityThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2..8°C.LimitationsoftheTestBacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.Inimmunocompromisedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.ReferencesCorey,L.;P.G.Spear:Infectionswithherpessimplexviruses.N.Engl.J.Med.314(1986)686-691Dörries,R.,R.Kaiser,H.Imrichetal.:NeuereAspektezurDiagnosezentralnervöserVirusinfektionen.Lab.Med.15(1991)99-102Felgenhauer,K.:DiagnostischeBedeutungderlokalsynthetisiertenspezifischenAntikörperdesLiquorcerebrospinalis.Lab.Med.15(1991)208Kaufmann,H.E.;F.MartolaandC.Dohlman:Theuseof5-iodo-2-deoxy-uridine(IDU)inthetreatmentofherpessimplexkeratitis.Arch.Ophthal.N.Y.68(1962)235-239Spaar,F.-W.:DiemenschlicheHerpessimplexEnzephaltitsundMeningitis.Eineklinisch-neurologischeUntersuchung.G.FischerVerlag,Stuttgart,NewYork(1976)AdditionalInformation:NameHerpessimplexVirus2(HSV2)IgGrecombinantantigensRelatedProductNamesHerpesSimplexVirus2(HSV2)IgG-ELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.IntendedUseResearchUseOnly

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