Description:Short Description: In this assay the HAPTOGLOBIN present in serum sample reacts with the anti-HAPTOGLOBIN antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hp in the test sample. The quantity of Hp in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.Additional Information: Name Pig Haptoglobin ELISA Kit Related Product Names Pig Haptoglobin ELISA Kit; N/A Pig HaptoglobinHP Gene id 397061 Alias Symbols Haptoglobin, HP, Zonulin Protein Name Haptoglobin Description of Target As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also a Swissprot ID Q8SPS7 Species Reactivity Pig Assay Info Assay Methodology: Quantitative Sandwich ELISA Sensitivity 3.107 ng/ml Kit Range 6.25 ng/ml - 400 ng/ml Homology Pig NCBI Acc Number NP_001119574.1 Standard Range Refer To Manual Sample Type Serum, plasma and other biological fluids Product Format In Solution Specimen/Volumes Refer To Manual Swiss Prot Number P00738 Applications ELISA Reconstitution and Storage 1. DILUENTThe 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-Hp conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-PIG HAPTOGLOBIN ELISA MICRO PLATEAnti-Pig Hp coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. PIG HAPTOGLOBIN CALIBRATORThe lyophilized Pig Haptoglobin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. Reactivity Pig Kit Size 96 well Kit Principle Genway Biotech Haptoglobin ELISA Kit (Pig) (GWB-6A5015) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Haptoglobin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Haptoglobin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Haptoglobin captured in the well. Kit Component ComponentAmount Anti-Pig Haptoglobin Microplate96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate50 mL 20X Wash Solution50 mL 100X Enzyme-Antibody Conjugate150 uL Chromogen-Substrate Solution12 mL Stop Solution12 mL Pig Haptoglobin Calibrator1 Kit Duration 40 Minutes Storage Refer to kit instructions for component storage Lead Time Domestic: within 1 week delivery International: 1 week Intended Use Research Use Only Additional Information Intended Use: The Haptoglobin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Haptoglobin in biological fluid of Pigs. :: Reagents: 1. DILUENT CONCENTRATE (Running Buffer)One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100XOne vial containing 150 ul of affinity purified anti-Pig Haptoglobin antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTIONOne vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTIONOne vial containing 12 ml 0.3 M sulfuric acid.WARNING: Avoid contact with skin.6. ANTI-PIG HAPTOGLOBIN ELISA MICRO PLATETwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Pig Hp.7. PIG HAPTOGLOBIN CALIBRATOROne vial containing a lyophilized Pig Haptoglobin calibrator.