Description:Species:HumanSampleType:Serum/PlasmaForthequantitativedeterminationofProgesteroneConcentrationinserum.Blood,Genitalia,EndocrineGlands,CellsCultured,CellularStructuresPleaseclickhereforMSDSPDFDatasheetProductName:ProgesteroneConcentrationSampleType:Serum/Plasma#ofSamples:1X96AssaysIntendedUse:ForthequantitativedeterminationofProgesteroneconcentrationinhumanserumorplasmaIntroduction:ProgesteroneisaC21steroidwhichissynthesizedfrombothtissueandcirculatingcholesterol.Cholesterolistransformedtopregnenolonewhichisthenconvertedviaacombineddehydrogenaseandisomerasetoprogesterone.Theprincipleproductionsitesaretheadrenalsandovariesandtheplacentaduringpregnancy.Themajorityofthissteroidismetabolizedinthelivertopregnanediolandconjugatedasaglucuronidepriortoexcretionbythekidneys.Progesteroneexhibitsawidevarietyofendorganeffects.Theprimaryroleofprogesteroneisexhibitedbythereproductiveorgans.Inmales,progesteroneisanecessaryintermediatefortheproductionofcorticosteroidsandandrogens.Infemales,progesteroneremainsrelativelyconstantthroughoutthefollicularphaseofthemenstrualcycle.Theconcentrationthenincreasesrapidlyfollowingovulationandremainselevatedfor4-6daysanddecreasestotheinitiallevel24hoursbeforetheonsetofmenstruation.Inpregnancy,placentalprogesteroneproductionrisessteADIlytolevelsof10to20timesthoseofthelutealphasepeak.Progesteronemeasurementsarethusperformedtodetermineovulationaswellastocharacterizelutealphasedefects.Monitoringofprogesteronetherapyandearlystagepregnancyevaluationscomprisetheremainingusesofprogesteroneassays.GenWayProgesteroneEIAkitsaredesignedforthemeasurementoftotalprogesteroneinhumanserumorplasma.Principleofthetest:TheGenWayprogesteroneEIAisbasedontheprincipleofcompetitivebindingbetweenprogesteroneinthetestspecimenandprogesterone-HRPconjugateforaconstantamountofrabbitanti-progesteroneIntheincubation,goatanti-rabbitIgG-coatedwellsareincubatedwith25?lprogesteronestandards,controls,patientsamples,100?lprogesterone-HRPConjugateReagentand50?lrabbitanti-progesteronereagentatroomtemperature(18-25C)for90minutes.Duringtheincubation,afixedamountofHRP-labeledprogesteronecompeteswiththeendogenousprogesteroneinthestandard,sample,orqualitycontrolserumforafixednumberofbindingsitesofthespecificprogesteroneantibody.Thus,theamountofprogesteroneperoxidaseconjugateimmunologicallyboundtothewellprogressivelydecreasesastheconcentrationofprogesteroneinthespecimenincreases.Unboundprogesteroneperoxidaseconjugateisthenremovedandthewellswashed.Next,asolutionofTMBReagentisthenaddedandincubatedatroomtemperaturefor20minutes,resultinginthedevelopmentofbluecolor.ThecolordevelopmentisstoppedwiththeadditionofStopSolution,andtheabsorbanceismeasuredspectrophotometricallyat450nm.Theintensityofthecolorformedisproportionaltotheamountofenzymepresentandisinverselyrelatedtotheamountofunlabeledprogesteroneinthesample.Astandardcurveisobtainedbyplottingtheconcentrationofthestandardversustheabsorbance.Theprogesteroneconcentrationofthespecimensandcontrolsrunconcurrentlywiththestandardscanbecalculatedfromthestandardcurve.StorageandStABIlity:Storeat2to8C.LimitationoftheTest:1.ReliableandreproducIBLeresultswillbeobtainedwhentheassayprocedureiscarriedoutwithacompleteunderstandingofthepackageinsertinstructionsandwithadherencetogoodlaboratorypractice.2.Thewashprocedureiscritical.Insufficientwashingwillresultinpoorprecisionandfalselyelevatedabsorbancereadings.3.Serumsamplesdemonstratinggrosslipemia,grosshemolysis,orturbidityshouldnotbeusedwiththistest.4.Theresultsobtainedfromtheuseofthiskitshouldbeusedonlyasanadjuncttootherdiagnosticproceduresandinformationavailabletothephysician.ProductName:ProgesteroneConcentrationAdditionalInformation:NameProgesteroneEnzymeImmunoassayTestKitRelatedProductNamesProgesteroneConcentrationELISAKitProgesteroneConcentrationIntendedUseResearchUseOnly