Description:ProductName:CA19-9#ofSamples:1x96AssaysIntendedUse:ImmunoenzymaticcolorimetricmethodforquantitativedeterminationofCA19-9inhumanserumorplasma.Introduction:AgroupofmucintypeglycoproteinSialosylLewisAntigens(SLA),suchasCA19-9andCA19-5,havecometoberecognizedascirculatingcancerassociatedantigensforgastrointestinalcancer.CA19-9representsthemostimportantandbasiccarbohydratetumorMarker.TheimmunohistologicaldistributionofCA19-9intissuesisconsistentwiththequantitativedeterminationofhigherCA19-9concentrationsincancerthaninnormalorinflamedtissues.RecentreportsindicatethattheserumCA19-9levelisfrequentlyelevatedintheserumofsubjectswithvariousgastrointestinalmalignancies,suchaspancreatic,colorectal,gastricandhepaticcarcinomas.TogetherwithCEA,elevatedCA19-9issuggestiveofgallbladderneoplasminthesettingofinflammatorygallbladderdisease.Thistumor-associatedantigenmayalsobeelevatedinsomenon-malignantconditions.ResearchstudiesdemonstratethatserumCA19-9valuesmayhaveutilityinmonitoringsubjectswiththeabove-mentioneddiagnosedmalignancies.IthasbeenshownthatapersistentelevationinserumCA19-9valuefollowingtreatmentmaybeindicativeofoccultmetastaticand/orresidualdisease.ApersistentlyrisingserumCA19-9valuemaybeassociatedwithprogressivemalignantdiseaseandpoortherapeuticresponse.AdecliningCA19-9valuemaybeindicativeofafavourableprognosisandgoodresponsetotreatment.Principlesoftheassay:MicrotiterstripwellsareprecoatedwithmonoclonalantibodiesagainstCA19-9.DuringfirstincubationCA19-9insamplesandstandardsbindtotheimmobilisedantibodiesonthesurfaceofthemicrotiterwells.Unboundsubstanceswillberemovedbyasubsequentwashingstep.Duringsecondincubationspecificanti-IgGantibodiesconjugatedwithperoxidasebindtoCA19-9.Unboundconjugatewillberemovedbyasubsequentwashingstep.InathirdincubationtheenzymesubstrateTMBwillbeoxidizedresultinginabluecolour.Additionofsulphuricacid(stopsolution)stopstheenzymaticreactionandturnsthebluereactionproductintoyellow.Absorbanceismeasuredat450nm.TheamountofoxidizedTMBisproportionaltotheamountofCA19-9inthesample.StorageandStABIlity:Theclosedreagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°Cinthedark.LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.ReferencesGlennJ.,etal.,J.Clin.Oncol.6:462-8(1988)HayakawaT.,etal.,Cancer61:1827-31(1988)KoprowskiH.,etal.,Science212:53-5(1981)MalesciA.,etal.,Gastroenteroglogy92:60-7(1987)SafiF,etal.,Pancreas2:398-403(1987)SteinbergW.,AmJ.ofGastroenterology85:350-355(1990)SteinbergW.M.,etal.,Gastroenterology90:343-9(1986)TakasakiH.,etal.,CancerRes.48:1435-8(1988)TatsutaM.,etal.,Cancer56:2669-73(1985)WangT.H.eta.,lPancreas1:219-23(1986)StromBL,etal.,Int.J.Cancer45:821(1990)AdditionalInformation:NameCA19-9RelatedProductNamesCA19-9ELISAMolecularWeight0.5StorageTheclosedreagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°Cinthedark.IntendedUseResearchUseOnly