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GenWay/Corynebacterium diphtheriae toxin 5S IgG (antitoxin) quantitative/GWB-73E000/1x96 Assays

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GWB-73E000

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Description:PleaseclickhereforMSDSPDFDatasheetProductName:CoryNEBacteriumdiphtheriaetoxin5SIgG-ELISA#ofSamples:1x96AssaysIntendedUse:TheCorynebacteriumdiphtheriaetoxin5SIgG-ELISAisintendedforthequantitativedeterminationofIgGclassantibodiesagainstCorynebacteriumdiphtheriaetoxininhumanserumorplasma(citrate).Thisallowsthedeterminationoftheimmunestatusofthepatientsfacilitatingindividualrecommendationsaboutthenecessityofabasicimmunizationorboosterinjection.Introduction:Corynebacteriaareaerobicnonspore-forminggram-positiverodsofirregularshape(0.51μmthickand2-6μmlong).Theycompriseskincommensals,opportuNISTpathogensandseveralmajorpathogens,includingCorynebacteriumdiphtheriae.Ingeneral,theyareisolatedfromthroatswabsonselectivemediacontainingtellurite.ThebacterialinfectioncausedbyC.diphtheriae,Diphtheria,hastwoforms.Respiratorydiphtheriaistypicallycausedbytoxin-producing(toxigenic)strains;cutaneousdiseasecanbecausedbyeithertoxigenicornontoxigenicstrains.Intherespiratoryformofthedisease,amembraneisformed;thismembraneisusuallyvisIBLeonthethroatortonsils.Personsmaydiefromasphyxiationwhenthemembraneobstructsbreathing.Othercomplicationsarecausedbyremoteeffectsofthediphtheriatoxin(myocarditis,nerveparalysis)Cutaneousdiphtheriaisusuallymild,typicallyconsistingofnon-distinctivesoresorshallowulcersandonlyrarelyinvolvingtoxiccomplications(1-2%ofinfectionswithtoxigenicstrains).Diphtheriawasoneofthemostcommoncausesofdeathamongchildrenduringtheprevaccineera.Sincetheintroductionandwidespreaduseofdiphtheriatoxoidvaccine(formalin-inactivateddiphtheriatoxin)inmostindustrializedcountriesthediseaseisnowcharacterizedbysporADIccasesandintermittentoutbreaksoflowintensity.ButrecentlargeepidemicsofdiphtheriainseveraleasternEuropeancountrieshaveagaindrawnattentiontothisforgottendiseaseand,themajorityofthesecaseshaveoccurredamongadolescentsandadultsinsteadofchildren.Theonlyeffectivewaytocontroldiphtheriaisbyprophylacticimmunizationwithdiphtheriatoxoid.Antibodytothetoxoidprotectsagainsttheactionofthetoxin;immunizedpersonscanbeinfectedbytoxin-producingstrainsofdiphtheria,butthesystemicmanifestationsofdiphtheriadonotoccur.Theoutcomeofthediseaseimproveswithearly,appropriatetreatment.Promptrecognitionandreportingofthediseaseisimportanttoassureearly,appropriatetreatmentwithdiphtheriaanti-toxin.InfectionmaybeidentifiedbyMicroscopy:GramstainSEROlogy:DetectionoftoxinproductionbyELISAPrinciplesoftheassay:ThequantitativeimmunoenzymaticdeterminationofIgG-classantibodiesagainstC.diphtheriaetoxinisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.MicrotiterstripwellsareprecoatedwithinactivatedspecificCorynebacteriumdiphtheriaetoxin(toxoid)antigenstobindcorrespondingantibodiesofthespecimen.Afterwashingthewellstoremoveallunboundsamplematerialhorseradishperoxidase(HRP)labelledanti-humanIgGconjugateisadded.ThisconjugatebindstothecapturedC.diphtheriaetoxin-specificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofC.diphtheriaetoxin-specificIgGantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat28°C.LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.Inimmunocompromizedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.ReferencesBGA,ImpfempfehlungderStändigenImpfkommission(STIKO)desBundesgesundheitsamtesvom22.2.1994,Bundesgesundheitsblatt8/94CDC,InternationalNotes(1993):DiphtheriaOutbreak-RussianFederation,1990-1993:MorbidityandMortalityWeeklyReport42:840Hofmann,F.,F.Schuh,M.Michaelis,U.Stößel(1994):ZurAkzetanzvonSchutzimpfungenbeiÄrztenundbeiderAllgemein-bevölkerung,Ges.Wes.56,371-376WHO(7.5.1993):ExpandedProgrammeonImmunization-Outbreakofdiphtheria,updateWklyEpidRecNo.19,134-138WHO(26.8.1994):ExpandedProgrammeonImmunization-DiphtheriaEpidemic.WklyEpidRecNr.34RKI(1999)PopulationsimmunitätgegenDiphtherieundPertussis.EpidemiologischesBulletin1/99,1-4AdditionalInformation:NameCorynebacteriumdiphtheriaetoxin5SIgG(antitoxin)quantitativeRelatedProductNamesCorynebacteriumdiphtheriaetoxin5SIgG-ELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat28°C.IntendedUseResearchUseOnly

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