首页 > GenWay > GenWay/Human beta 2-Microglobulin ELISA Kit/GWB-75DA03/96 well

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GenWay/Human beta 2-Microglobulin ELISA Kit/GWB-75DA03/96 well

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GWB-75DA03

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AdditionalInformation:NameHumanbeta2-MicroglobulinELISAKitRelatedProductNamesHumanbeta2-MicroglobulinELISAKitGeneSymbolB2MGeneFullnamebeta-2-microglobulinGeneid567AliasSymbolsBeta-2-microglobulin,B2MProteinNameBeta-2-microglobulinDescriptionofTargetComponentoftheclassImajorhistocompatibilitycomplex(MHC).Involvedinthepresentationofpeptideantigenstotheimmunesystem.ExogenouslyappliedM.tuberculosisEsxAorEsxA-EsxB(orEsxAexpressedinhost)bindsB2ManddecreasesitsexporttoSwissprotIDP61769SpeciesReactivityHumanAssayInfoAssayMethodology:QuantitativeSandwichELISASensitivity0.822ng/mlKitRange3.125ng/ml-100ng/mlHomologyHumanStandardRangeReferToManualSampleTypeSerum,plasmaandotherBIOLOGicalfluidsProductFormatInSolutionSpecimen/VolumesReferToManualApplicationsELISAReconstitutionandStorage1.DILUENTThe5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.2.WASHSOLUTIONThe20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.3.ENZYME-ANTIBODYCONJUGATEUndilutedhorserADIshperoxidaseanti-B2Mconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.4.CHROMOGEN-SUBSTRATESOLUTIONTheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.5.STOPSOLUTIONTheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.6.ANTI-HUMANB2MELISAMICROPLATEAnti-HumanB2Mcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.7.HUMANB2MCALIBRATORThelyophilizedHumanBeta2-Microglobulincalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforuptoonehour.ReactivityHumanKitSize96wellKitPrincipleGenWayBiotechbeta2-MicroglobulinELISAKit(Human)(GWB-75DA03)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforBeta-2-microglobulinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Beta-2-microglobulin-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleBeta-2-microglobulincapturedinthewell.KitComponentComponentAmountAnti-Humanbeta2-MicroglobulinMicroplate96Wells(12x8WellStrips)20XDiluentConcentrate50mL20XWashSolution50mL100XEnzyme-AntibodyConjugate150uLChromogen-SubstrateSolution12mLStopSolution12mLHumanbeta2-MicroglobulinCalibrator1KitDuration130MinutesStorageRefertokitinstructionsforcomponentstorageLeadTimeDomestic:within1weekdelivery International:1weekIntendedUseResearchUseOnlyAdditionalInformationIntendedUse:1.DILUENTCONCENTRATE(RunningBuffer)Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.2.WASHSOLUTIONCONCENTRATEOnebottlecontaining50mlofa20Xconcentratedwashsolution.3.ENZYME-ANTIBODYCONJUGATE100XOnevialcontaining150ulofaffinitypurifiedanti-HumanBeta2-MicroglobulinantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer.4.CHROMOGEN-SUBSTRATESOLUTIONOnevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.5.STOPSOLUTIONOnevialcontaining12ml0.3Msulfuricacid.WARNING:Avoidcontactwithskin.6.ANTI-HUMANB2MELISAMICROPLATETwelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-HumanB2M.7.HUMANB2MCALIBRATOROnevialcontainingalyophilizedHumanBeta2-Microglobulincalibrator.::Reagents:1.DILUENTCONCENTRATE(RunningBuffer)Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.2.WASHSOLUTIONCONCENTRATEOnebottlecontaining50mlofa20Xconcentratedwashsolution.3.ENZYME-ANTIBODYCONJUGATE100XOnevialcontaining150ulofaffinitypurifiedanti-HumanBeta2-Microglobulinantibodyconjugatedwithhorseradishperoxidaseinastabilizingbuffer.4.CHROMOGEN-SUBSTRATESOLUTIONOnevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.5.STOPSOLUTIONOnevialcontaining12ml0.3Msulfuricacid.WARNING:Avoidcontactwithskin.6.ANTI-HUMANB2MELISAMICROPLATETwelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-HumanB2M.7.HUMANB2MCALIBRATOROnevialcontainingalyophilizedHumanBeta2-Microglobulincalibrator.

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