Description:Angiogenesis provides the crucial link between the avascular and vascular states and is a key event for sustained tumor growth and cancer progression. Angiogenesis as a biological switch process is governed by numerous pro- and anti-angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGFb), epidermal growth factor (EGF), and transforming growth factor-beta (TGF-b). The mechanism of action of each of these factors is different, as are their origin and the stimuli for their production. The angiogenic switch refers to the balance between pro- and anti- angiogenic factors. Therefore, profiling of these factors is critical to understanding angiogenesis. Signosis’ Angiogenesis ELISA Strip I Profiling Assay allows simultaneously profiling 8 angiogenesis cytokines. Each well of the strip is coated with a primary antibody against a specific angiogenesis protein and total 8 wells of a strip target 8 different proteins. The difference of these proteins between two samples can be determined through data comparison.Principle:Each well of the strip is coated with a specific capture antibody to detect its corresponding cytokine in the sample. Therefore, 8 different proteins can be measures simultaneously. The test sample reacts simultaneously with two antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The HRP substrate, TMB, is then added and causes a blue color change. The reaction is then terminated with Stop Solution, resulting in a yellow color. The concentrations of oxidative stress cytokines are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. The expression levels of these cytokines can be quantitatively compared between samples.       Data:                                 Human Angiogenesis ELISA strip I for profiling 8 cytokines. HeLa cells were treated with (red bars) or without (light blue) 10ng/ml TNF. Conditioned media were collected (top) and cell lysates were prepared (bottom) for ELISA strip profiling.