Note: The product is for research use only, not for use in diagnostic or therapeutic procedures Product Name Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody Synonyms CEK5 ligand; CEK5 receptor ligand; CEK5-L; CEL5-L; EFL-3; EFNB1; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; EPL2; EPLG2; Ephrin-B1 precursor; LERK-2; LERK2; STRA1; STRA1 protein; kinase ephrin-B1 Immunogen The antiserum was produced against synthesized peptide derived from human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324. Immunogen Range 290-339 Clonality Polyclonal Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 Specificity Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at Tyr324. Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage/Stability Stable at -20°C for at least 1 year. NCBI Gene Symbol EFNB1 Database Links Gene ID: 1947/1948/1949 Swiss-Prot #: P98172/P52799/Q15768 OMIM #: 300035/304110/600527/602297 Unigene #: Hs.144700/Hs.149239/Hs.26988 Target Modification Phospho Modification Sites H:Y324/311/318 M:Y323/314/318 R:Y323/-/318 Application Images Image 1 Immunohistochemistry analysis of paraffin-embedded human brain, using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The picture on the right is blocked with the phospho peptide. Image 2 Western blot analysis of lysates from K562 cells treated with serum 20% 15', using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The lane on the right is blocked with the phospho peptide.