产品概述 This ELISPOT assay is designed to enumerate Perforin producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing perforin production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of perforin producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.Principal:After cell stimulation, locally produced cytokines or molecule are captured by a specific monoclonal antibody. After cell lysis, trapped specific soluble molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored \"purple\" spots indicate cytokine production by individual cells.Recognizes natural human Perforin Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. Perforin is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The Abpromise guarantee Abpromise™承诺保证使用ab62943于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。 发表研究结果有使用 ab62943？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab62943 尚未被引用在任何文献中。 There are currently no Customer reviews or Questions for ab62943.Please use the links above to contact us or submit feedback about this product.Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com