存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
存储溶液 Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
纯化说明 This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
常规说明
Alexa Fluor is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Abpromise guarantee
Abpromise™承诺保证使用ab150079于以下的经测试应用
\"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
ICC/IF 1/200 - 1/1000. ELISA Use at an assay dependent concentration. IHC-P Use at an assay dependent concentration. Flow Cyt 1/2000. 说明 IHC-Fr
Use at an assay dependent concentration. ICC/IF
1/200 - 1/1000. ELISA
Use at an assay dependent concentration. IHC-P
Use at an assay dependent concentration. Flow Cyt
1/2000. 图片 Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
ICC/IF image of ab6046 in HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 2 g/ml) overnight at +4 C. The secondary antibody ab150079 (shown in red) was used at 1 g/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 M.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 g/ml(50 l/well) and incubatedovernight at 4 C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 g/ml and gradually diluted 1/4 (50 l/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H L (HRP)(ab6885) was used at 1/10,000 dilution (50 l/well), followed by incubationfor 1h at RT.
For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, RatIgG and Chicken IgY.
This data was developed using the unconjugated antibody (ab182016).
Cross-reactivity of Goat anti-Rabbit IgG H L (ab182016)and Goat anti-Rabbit IgG H Lobtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 g/ml (50 l/well) and incubatedovernight at 4 C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 g/ml and gradually diluted 1/4 (50 l/well), followed by incubationfor 2h. For the detection Donkey anti-Goat IgG H L (HRP)(ab6885) was used at 1/10,000 dilution (50 l/well), followed by incubationfor 1h atRT. This data is from a representative dilution.
This data was developed using the unconjugated antibody (ab182016).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Click here to view the general protocols
发表研究结果有使用 ab150079？请让我们知道，以便我们可以引用本数据表中的参考文章。
ab150079 被引用在 146 文献中.
Wu F et al. Bleomycin A5 suppresses Drp1-mediated mitochondrial fission and induces apoptosis in human nasal polyp-derived fibroblasts. Int J Mol Med 47:346-360 (2021).PubMed: 33236140 Nishida Y et al. Intra-Articular Injection of Stromal Cell-Derived Factor 1a Promotes Meniscal Healing via Macrophage and Mesenchymal Stem Cell Accumulation in a Rat Meniscal Defect Model. Int J Mol Sci 21:N/A (2020).PubMed: 32751701 Xi K et al. Microenvironment-responsive immunoregulatory electrospun fibers for promoting nerve function recovery. Nat Commun 11:4504 (2020).PubMed: 32908131 Liu YM et al. A Carbohydrate-Binding Protein from the Edible Lablab Beans Effectively Blocks the Infections of Influenza Viruses and SARS-CoV-2. Cell Rep 32:108016 (2020).PubMed: 32755598 Li Y et al. Complement 3 mediates periodontal destruction in patients with type 2 diabetes by regulating macrophage polarization in periodontal tissues. Cell Prolif 53:e12886 (2020).PubMed: 32794619 View all Publications for this productE10.5 mouse embryo section. Samples were fixed with PFA and stained before antigen retrieval with sodium citrate.
Primary: FOXA2 ab40874 (1:200).
Secondary: Alexa Fluor 647 ab150079 (1:1000).
Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
I have read the details you have kindly provided and have following further questions for better understanding of the problem;
- Have you used correct filters? The ab150079 absorption maxima is 650 nm while for FITC (ab98692) it is 495.
- Were the cells permeabilized?
- Is the sequence of insert protein is interpreted. It may well be that the insert sequence is missing which ab128251 binds.
The antibodies ab128251 and a84815 are raised against different part of the immunogen.
- Have you tried optimizing the results by incubating the antibody overnight or by increasing the concentration?
In order for checking the quality of ab150079 you may put a small drop of ab on slide and then seeing it under microscope. If it emits red colour then conjugate is fine and the problem may be with other steps. If it\'s not then problem is with secondary antibody.
It will be very helpful if you could provide the protcol followed step by step.
Please note that flow cytometry is not a tested application for ab128251 so the Abreview guarantee will not be valid. However if this antibody fails in ELISA, WB and ICC/IF then guarantee will apply as normal product.
Thank you very much for your cooperation. I will look forward to hearing from you soon.
Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES
For licensing inquiries, please contact partnerships@abcam.com
