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Wako湖南代理
热烈庆祝上海蚂蚁淘科技有限公司成为Wako湖南代理,感谢Wako公司对我公司的信任和支持!我公司现在签约代理品牌超过20家,品牌来自美国,德国,法国,英国,加拿大以色列等欧美国家,产品种类齐全。一站式采购专家:官网:http://www.yubiotech.com|商城:http://www.ybiotechmall.comWako湖南代理,简介:蚂蚁淘公司做为Wako湖南代理,专业经营原装进口胎牛血清细胞因子ELISA试剂盒、细胞、抗体、生物试剂、耗材培养基一抗、二抗等。Wako湖南代理的产品涉及分子生物学、细胞生物学、**学、遗传学、**学、生物化学、蛋白质学、细胞**、临床应用等领域。做为Wako湖南代理,我们郑重承诺:保证原装进口,如出现运输质量问题,我们可以包退换货。Wako湖南代理,所能提供产品如下:QuantiTect Multiplex PCR Kit (1000),货号:Qiagen 204545;1x 25 ml Master Mix1x 20 ml RNase free waterEZ1 DNA BACT Card,货号:Qiagen 9016362;QIAprep Spin Miniprep Kit (1000),货号:Qiagen 27106X4;QIAquick PCR Purification Kit (1000),货号:Qiagen 28106X4;5-hole tube units (256),货号:Qiagen 19589;MagAttract Direct mRNA M48 Kit (192),货号:Qiagen 957336;QuantiTect Multiplex PCR NoRox Kit(1000),货号:Qiagen 204745;HotStarTaq Master Mix Kit (2500),货号:Qiagen 203446;1x25 ml HotStarTaq Master Mix (providing a final concentration of 1.5mM MgCl2, and 200 uM each dNTP), containing 2500 units HotStarTaq DNA Polymerase total, and 2 x 20 ml RNase-free Water- Please note 2005 Product guide is incorrect - this product is NOT 30vials of 0.85mlTaq DNA Polymerase (25000),货号:Qiagen 201209;100 x 250 units Taq DNA Polymerase, 100 x 1.2ml PCR Buffer, 100 x 2.0ml Q-Solution, 100 x 1.2ml MgCl2NEW! Now with 100 x 1,2 ml CoralLoad PCR BufferTaq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time.HotStarTaq DNA Polymerase (25000),货号:Qiagen 203209;HotStarTaq DNA Polymerase Kit (25000)100 x 250 units HotStarTaq DNA Polymerase, 100 x 1.2ml HotStarTaq Buffer, 100 x 2.0ml Q-Solution, 100 x 1.2ml MgCl2Old SAP # 1009302Qproteome Mannose Glycoprotein Kit,货号:Qiagen 37551;HiPerFect Transfection Reagent (0,5 ml),货号:Qiagen 301704;HiPerFect Transfection Reagent (4x1 ml),货号:Qiagen 301707;PAXgene Blood RNA MDx Kit (4),货号:Qiagen 762431;BioSprint 15 DNA Plant Kit (360),货号:Qiagen 941517;BioSprint 96 DNA Plant Kit (576),货号:Qiagen 941557;MagAttract RNA Tissue Mini M48 Kit (192),货号:Qiagen 959236;QuantiTect Rev.Transcription Kit (50),货号:Qiagen 205311;For 50 x 20 μl reactions: gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, RNase-Free Water.Suitable only for cDNA synthesis followed by real-time analyses.QuantiTect Primer Assay (200),货号:Qiagen 249900;For 200 x 50 μl reactions: lyophilized mix of forward and reverse primers for real-time analysis with SYBR Green detection.Ni-NTA Fast Start Kit (6),货号:Qiagen 30600;Qproteome Nuclear Protein Kit,货号:Qiagen 37582;Qproteome Murine Albumin Depletion Kit,货号:Qiagen 37591;Qproteome Bacterial Protein Prep Kit,货号:Qiagen 37900;MagAttract RNA Cell Mini M48 Kit (192),货号:Qiagen 958236;HiPerFect Transfection Reagent (0,1ml),货号:Qiagen 301702;TissueLyser Single-Bead Dispenser, 5mm,货号:Qiagen 69965;TissueLyser Single-Bead Dispenser, 7mm,货号:Qiagen 69967;PhosphoProtein Lysis Buffer (1000ml),货号:Qiagen 1031358;Disposable Tips, 50μl (960),货号:Qiagen 990012;CompactPrep Plasmid Midi Kit (25),货号:Qiagen 12843;For ultrafast purification of up to 200 μg or 750 μg Molecular Biology Grade plasmid or cosmid DNA for standard applicationsCompactPrep Plasmid Kits enable fast, large-scale plasmid purification in a miniprep format. The use of a vacuum manifold allows 24 samples to be purified in parallel on a single workbenchCompactPrep Plasmid Maxi Kit (25),货号:Qiagen 12863;For ultrafast purification of up to 200 μg or 750 μg Molecular Biology Grade plasmid or cosmid DNA for standard applicationsCompactPrep Plasmid Kits enable fast, large-scale plasmid purification in a miniprep format. The use of a vacuum manifold allows 24 samples to be purified in parallel on a single workbenchTurboCapture 96 mRNA Kit (5),货号:Qiagen 72251;For rapid and easy mRNA purification in high-throughput applications. The kit provides a fast and simple procedure for purifying mRNA. Up to 96 samples can be processed simultaneously - ideal for high-throughput applications such as compound screening and target validation (e.g. by siRNA screening).TurboCapture 384 mRNA Kit (5),货号:Qiagen 72271;For rapid and easy mRNA purification in high-throughput applications. The kit provides a fast and simple procedure for purifying mRNA. Up to 384 samples can be processed simultaneously - ideal for high-throughput applications such as compound screening and target validation (e.g. by siRNA screening).Random Hexamers (100 μl),货号:Qiagen 79236;100 ul of random hexamers (0.4 ug/ul) for cDNA synthesis with TurboCapture mRNA KitsOligo-dt Primers (100 μl),货号:Qiagen 79237;Buffer TCL (125/125),KG,货号:Qiagen 1031576;EZ1 RNA Universal Tissue Kit (48),货号:Qiagen 956034;Provides automated purification of total RNA from 1-6 samples of all types of animal or human tissue, including difficult-to-lyse fibrous and fatty tissues, or cultures cells.EZ1 RNA Cell Mini Kit (48),货号:Qiagen 958034;general purpose device,48 Reagent Cartridges (RNA Cell) 100 Disposable Tip Holders, 100 Disposable Filter-Tips, 50 Sample Tubes (2 ml), 50 Elution Tubes (1.5 ml), Buffer RLT, RNase-Free DNase IREPLI-g Mini Kit (25),货号:Qiagen 150023;DNA Polymerase, Buffers, and Reagents for 25 x 50 μl whole genome amplification reactions (typical yield 10 μg per reaction)REPLI-g Midi Kit (25),货号:Qiagen 150043;DNA Polymerase, Buffers, and Reagents for 25 x 50 μl whole genome amplification reactions (typical yield 40 μg per reaction)REPLI-g Mini Kit (100),货号:Qiagen 150025;DNA Polymerase, Buffers, and Reagents for 100 x 50 μl whole genome amplification reactions (typical yield 10 μg per reaction)REPLI-g Midi Kit (100),货号:Qiagen 150045;DNA Polymerase, Buffers, and Reagents for 100 x 50 μl whole genome amplification reactions (typical yield 40 μg per reaction)HotStarTaq Plus DNA Polymerase (250),货号:Qiagen 203603;1 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2HotStarTaq Plus DNA Polymerase (1000),货号:Qiagen 203605;4 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2HotStarTaq Plus DNA Polymerase (5000),货号:Qiagen 203607;1 x 5000 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2QuantiTect Multiplex RT-PCR Kit (200),货号:Qiagen 204643;QuantiTect Multiplex RT-PCR Kit (1000),货号:Qiagen 204645;QuantiTect Multiplex RT-PCR NR Kit (200),货号:Qiagen 204843;HotStar HiFidelity Polymerase Kit (100),货号:Qiagen 202602;100 units HotStar HiFidelity DNA Polymerase, 5x PCR Buffer containing dNTPs, 5x Q-Solution, 25 mM MgSO4, RNase free waterHotStar HiFidelity Polymerase Kit (1000),货号:Qiagen 202605;2 x 500 units HotStar HiFidelity DNA Polymerase, 5x PCR Buffer containing dNTPs, 5x Q-Solution, 25 mM MgSO4, RNase free water

  上海蚂蚁淘——大鼠视网膜müller细胞|细胞系|细胞株|肿瘤细胞,公司不仅有大量的上等细胞,还提供大鼠视网膜müller细胞的全程后期服务,可以向专业人士咨询详细的大鼠视网膜müller细胞|细胞培养条件,冻存方法,及相关培养技术

  对于贴壁细胞,传代可参考以下方法:

  1. 弃去培养上清,用不含钙、镁离子的PBS润洗细胞1-2次。

  2. 加2ml消化液(0.25%Trypsin-0.53mM EDTA)于培养瓶中,置于37℃培养箱中消化1-2分钟,然后在显微镜下观察大鼠视网膜müller细胞|细胞消化情况,若细胞大部分变圆并脱落,迅速拿回操作台,轻敲几下培养瓶后加少量培养基终止消化。

  3. 按6-8ml/瓶补加培养基,轻轻打匀后吸出,在1000RPM条件下离心4分钟,弃去上清液,补加1-2mL培养液后吹匀。

  4. 将细胞悬液按1:2到1:5的比例分到新的含8ml培养基的新皿中或者瓶中。

  对于悬浮细胞,传代可参考以下方法:

  方法一:收集细胞,1000RPM条件下离心4分钟,弃去上清液,补加1-2mL培养液后吹匀,将细胞悬液按1:2到1:5的比例分到新的含8ml培养基的新皿中或者瓶中。

  方法二:可选择半数换液方式,弃去半数培养基后,将剩余细胞悬起,将细胞悬液按1:2到1:3的比例分到新的含8ml培养基的新皿中或者瓶中。

  3)细胞冻存:待细胞生长状态良好时,可进行大鼠视网膜müller细胞|细胞冻存。贴壁细胞冻存时,弃去培养基后加入少量胰酶,细胞变圆脱落后,加入约1ml含血清的培养基后加入冻存管中,再添加10%DMSO后进行冻存。悬浮细胞冻存时,应将细胞收集,1000RPM条件下离心4分钟,少量保存上清液(防止细胞吸走),加入部分新鲜培养基,加入到冻存管中,在冻存管中加入10%DMSO后进行冻存。

  大鼠视网膜müller细胞 蚂蚁淘|复旦细胞库国内各地均可发货,国内统一价格,本公司出售的所有细胞仅供科研使用。

  以下为公司部分与大鼠视网膜müller细胞相关细胞株:

  大鼠胰腺星状细胞|;大鼠真皮成纤维细胞|;大鼠正常肝细胞|;大鼠主动脉内皮细胞|;大隐静脉平滑肌细胞|;非小细胞肺癌|;非洲绿猴肾细胞|;宫颈癌细胞|;骨骼肌细胞|;骨髓瘤细胞|;骨细胞|;关节炎滑膜成纤维细胞|;冠状动脉内皮细胞|;冠状动脉平滑肌细胞|;海马神经元细胞|;颌下腺上皮细胞|;人恶性黑色素瘤细胞|;人非小细胞肺癌腺癌细胞|;人肺癌紫杉醇耐药株|;人肺成纤维细胞|;人肺大静脉内皮细胞|;人肺鳞癌细胞|;人肺泡上皮细胞|;人肝癌细胞株|;人肝动脉内皮细胞|;人肝母细胞瘤细胞|; Calu-3细胞|;Caski细胞|;CD34细胞|;CD4细胞|;CD8+细胞|;CD8细胞|;CHO-K1细胞|;CHO细胞|;CI-1细胞|;CIK细胞|;CI细胞|;CNE1细胞|;COLO205细胞|;COS7细胞|;CV-1细胞|;CX-1细胞|;DF-1细胞|;E14细胞|;EB-1细胞|;EM-3细胞|;F-2B细胞|;Foxp3细胞|;G1细胞|;H1299细胞|;H446细胞|;H9C2细胞|;HAb18Gedomab2细胞|;HCCLM3细胞|;HCT 116细胞|; HCT116细胞|;HCT-8细胞|;HEC-1-A细胞|;HEK293A细胞|;HEK293-T细胞|;HEK293细胞|;HEK-293细胞|;Hela S3细胞|;HeLaS3细胞|;HeLa细胞|;Hep3b细胞|;HepG2.2.1.5细胞|;HepG2.2.15细胞|;HepG2细胞|;HER-2细胞|;HL细胞|;HT22细胞|;HT-3细胞|;HT细胞|;HUVEC细胞|;

  本次列举细胞数量有限,欢迎进入蚂蚁淘公司网站详细了解大鼠视网膜müller细胞等其他细胞|细胞株的介绍。

  蚂蚁淘实验室专业人士提醒广大科研实验者,购买大鼠视网膜müller细胞培养,注意事项

  1. 收到细胞后,若发现干冰已挥发干净、冻存管瓶盖脱落、破损及细胞有污染,请立即与我们联系(51875103)。  

2. 所有动物细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护,所有废液及接触过此细胞的器皿需要**后方能丢弃。

  此外,如若存在任何疑问,欢迎来电或在线咨询(QQ:800040980),我们有专业客服为您提供上等服务。

Wako湖南代理
品牌产品名称规格价格货期
Wako湖南代理Cytokeratin8(human),mAb(35betaH11)(Ready-to-Use)100ug2001元部分现货
Wako湖南代理Cytokeratin8(human),mAb(35betaH11)100ug2002元部分现货
Wako湖南代理Cytokeratin1/5/10/14(human),mAb(34betaE12)(Ready-to-Use)100ug2003元部分现货
Wako湖南代理Cytokeratin1/5/10/14(human),mAb(34betaE12)100ug2004元部分现货
Wako湖南代理Cytokeratin1(human),mAb(34betaB4)(Ready-to-Use)100ug2005元部分现货

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