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Methods for the Detection of D-Amino-Acid Oxidase
AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described. References1.Krebs, H.A. 1935. CXCVII. Metabolism of amino-acids. III. Deamination of amino-acids. Biochem. J. 29, 1620–1644.PubMed  CAS Google Scholar  2.Meister, A. 1965. Biochemistry of the Amino Acids, 2nd ed, vol. 1. Academic Press, New York. Google Scholar  3.Konno, R., Yasumura, Y. 1992. D-Amino-acid oxidase and its physiological function. Int. J. Biochem. 24, 519–524.PubMed  Article  CAS Google Scholar  4.Watanabe, T., Motomura, Y., Suga, T. 1978. A new colorimetric determination of D-amino acid oxidase and urate oxidase activity. Anal. Biochem. 86, 310–315.PubMed  Article  CAS Google Scholar  5.Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.PubMed  CAS Google Scholar  6.Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.PubMed  Article  CAS Google Scholar  7.Chomczynski, P., Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–159.PubMed  Article  CAS Google Scholar  8.Sambrook, J., Fritsch, E.F., Maniatis, T. 1989. Molecular cloning: a laboratory manual., 2nd ed. Cold Spring Harbor Laboratory Press, New York. Google Scholar  9.Feinberg, A.P., Vogelstein, B. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13.PubMed  Article  CAS Google Scholar  10.Konno, R., Yasumura, Y. 1981. Activity and substrate specificity of D-amino acid oxidase in kidneys of various animals. Zool. Mag., Tokyo 90, 368–373.CAS Google Scholar  11.Konno, R., Uchiyama, S., Yasumura, Y. 1982. Intraspecies and interspecies variations in the substrate specificity of D-amino acid oxidase. Comp. Biochem. Physiol. 71B, 735–738.CAS Google Scholar  12.Konno, R., Yamamoto, K., Niwa, A., Yasumura, Y. 1991. Presence of D-amino-acid oxidase protein in mutant mice lacking D-amino-acid oxidase activity. Int. J. Biochem. 23, 1301–1305.PubMed  Article  CAS Google Scholar  13.Konno, R., Sasaki, M., Asakura, S., Fukui, K., Enami, J., Niwa, A. 1997. D-Amino-acid oxidase is not present in the mouse liver. Biochim. Biophys. Acta 1335, 173–181.PubMed  CAS Google Scholar  14.Konno, R. 1998. Rat D-amino-acid oxidase cDNA: rat D-amino-acid oxidase as an intermediate form between mouse and other mammalian D-amino-acid oxidases. Biochim. Biophys. Acta 1935, 165–170. Google Scholar  15.Nakano, S. 1961. Distribution of D-amino acid oxidase in mammalian tissues (in Japanese). Vitamin 22, 306–312.CAS Google Scholar  16.Katagiri, M., Tojo, H., Horiike, K., Yamano, T. 1991. Immunochemical relationship of D-amino acid oxidase in various tissues and animals. Comp. Biochem. Physiol. 99B, 345–350.CAS Google Scholar  17.Sasaki, M., Konno, R., Nishio, M., Niwa, A., Yasumura, Y., Enami, J. 1992. A single-base-pair substitution abolishes D-amino-acid oxidase activity in the mouse. Biochim. Biophys. Acta 1139, 315–318.PubMed  CAS Google Scholar  18.Konno, R., Yasumura, Y. 1983. Mouse mutant deficient in D-amino acid oxidase activity. Genetics 103, 277–285.PubMed  CAS Google Scholar  19.Konno, R., Yasumura, Y. 1984. Brain and kidney D-amino acid oxidases are coded by a single gene in the mouse. J. Neurochem. 42, 584–586.PubMed  Article  CAS Google Scholar  Download referencesAuthor informationAffiliationsDepartment of Microbiology, Dokkyo University School of Medicine, 321-0293, Mibu, Tochigi, JapanRyuichi KonnoAuthorsRyuichi KonnoView author publicationsYou can also search for this author in PubMed Google ScholarCorresponding authorCorrespondence to Ryuichi Konno.Rights and permissions This article is published under an open access license. Please check the \'Copyright Information\' section either on this page or in the PDF for details of this license and what re-use is permitted. If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and re-use information, please contact the Rights and Permissions team. About this articleCite this articleKonno, R. Methods for the detection of d-amino-acid oxidase. Biol Proced Online 1, 27–31 (1998). https://doi.org/10.1251/bpo7Download citationIssue Date: May 1998DOI: https://doi.org/10.1251/bpo7KeywordsOxidase ActivityCrucian CarpNorthern HybridizationTissue Culture CellBiological Procedure