SYPRO Ruby染色（左图），Anti-pTyr抗体进行western blot检测（右图）。结果显示使用Phos-tag™ Agarose可高效纯化磷酸化蛋白。
2、悬浮液在4度中，可保存至少半年；
3、Phos-tagTM与琼脂糖（Agarose）结合利用磷酸化基团与金属离子螯合的Phos-tagTM Agarose特异性结合，纯化磷酸化蛋白。
可用于亲和层析法进行磷酸化蛋白纯化。
Phos-tag® Agarose为分离、浓缩天然磷酸化蛋白和生物样品中磷酸化多肽提供一种高效的方法。
提供各种规格产品，以便用于不同样品的检测。
缓冲液不含有还原剂和去污剂。与亲和层析方法类似。
Phos-tag® Agarose捕获结合到Tyr、Thr、Ser、Asp、His等氨基酸，糖类、脂类上的无机磷酸根和大量二价磷酸根。
过氧化氢特异性荧光探针BES-H2O2-Ac / BES-H 2O2• 比二氯荧光黄 (DCFH) 有更高的特异性• 可动态监测活细胞内的过氧化氢• BES-H2O2-Ac 可穿透细胞膜，无需打孔• 极易溶于水，可制成水溶液使用• 可用于荧光显微镜、流式细胞仪BES-So-AM：使用举例图1）是Jurkat T细胞在含33μM BES-So-AM的培养基中37°C培养1小时，使探针进入细胞，然后添加4mM丁酸（O2•-诱导剂），37°C培养1小时；图2）是Jurkat T细胞在含33μM BES-So-AM的培养基中37°C培养1小时，使探针进入细胞，然后不添加丁酸，37°C再培养1小时；图3）是Jurkat T细胞在含33μM BES-So-AM的培养基中37°C培养1小时，然后添加5mM的丁酸，继续培养一个小时。参考文献1) Maeda, H. et al.: Angew. Chem. Int. Ed., 43, 239 (2004).2) Maeda, H. et al.: Chem. Pharm. Bull., 49, 294 (2001).1. Features•Much higher selectivity compared to conventionally-used DCFH probes•Responds specifically to hydrogen peroxide, enabling detection of intracellular hydrogen peroxide activity•BES-H2O2-Ac is cell membrane permeable•Superior water solubility allows use with aqueous solutions•Can be used in flow cytometry
Fluorescent images of Jurkat T cells with BES- H2O2 -Ac and the same-fi eld phase-contrast imagesJurkat T cells were cultured in a medium with 50μM BES-H2O2-Ac for 1 hour. Then, one group of them was cultured in a medium with 5 mM butyric acid (H2O2-produced stimulation), whereas the other in a medium without butyric acid (No H2O2 produced stimulation), each for 1 hour. (Courtesy: Professor Hatsuo Maeda, PhD., School of Pharmacy, Hyogo Univ. of Health Sciences) Fluorescent probe for hydrogen peroxide based on a non-oxidative mechanism , Maeda, H. et al.: Angew. Chem. Int. Ed., 43, 2389-91 (2004). Hydrogen peroxide-induced deacetylation of acetyl resorufin as a novel indicator reaction for fluorometric detection of glucose using only glucose oxidase , Maeda, H. et al.: Chem. Pharm. Bull., 49, 294-8 (2001).4. Products List
All products are for Cellbiology; Keep at room temperature
Heptaethylene Glycol Monododecyl Ether Standard Solution (1mg/ml Methanol Solution) 十二烷基七聚乙二醇醚Halogenated Acetic Acid Mixture Standard Solution (each 1mg/ml t-Butyl Methyl Ether Solution) 卤化醋酸标准溶液Dichloroacetonitrile Standard Solution (1mg/ml Acetonitrile Solution) 氰化二氯甲烷标准溶液Dichloroacetic Acid Standard Solution (1mg/ml t-Butyl Methyl Ether Solution) 二氯乙酸标准溶液trans-1,2-Dichloroethylene Standard Solution (1mg/ml Hexane Solution) 反-1，2-二氯乙烯标准溶液trans-1,2-Dichloroethylene Standard Solution (1mg/ml Methanol Solution) 反-1，2-二氯乙烯标准溶液trans-1,2-Dichloroethylene Standard Solution (1mg/ml Methanol Solution) 反-1，2-二氯乙烯标准溶液cis-1,2-Dichloroethylene Standard Solution (1mg/ml Methanol Solution) 顺-1，2-二氯乙烯标准溶液Chloroacetic Acid Standard Solution(1ml/mg t-Butyl Methyl Ether Solution) 氯乙酸标准溶液5 Chlorinated Phenols Mixture Standard Solution (each 1mg/ml in Acetone Solution) 5钟氯化酚混合物标准溶液3 Chlorinated Acetic Acids Mixutre Standard Solution (each 1mg/ml t-Butyl Methyl Ether Solution) 3种氯化醋酸混合物标准溶液3 Chlorinated Acetic Acid Methyl Esters Mixture Standard Solution (each 1mg/ml t-Butyl Methyl Ether Solution) 3种氯化醋酸甲酯混合物标准溶液Chloral Hydrate Standard Solution (1mg/ml t-Butyl Methyl Ether Solution) 水合氯醛标准溶液p-Bromofluorobenzene-Fluorobenzene Mixture Standard Solution (each 1mg/ml Methanol Solution) 对溴氟苯-氟苯混合物标准溶液Anionic Surfactants Mixture Standard Solution (each 1mg/ml Methanol Solution) 阴离子表面活性剂混合物标准溶液 糖苷为单糖半缩醛羟基与另一个分子（例如醇、糖、嘌呤或嘧啶）的羟基、胺基或巯基缩合形成的含糖衍生物。糖苷广泛分布于植物的根、茎、叶、花和果实中。大多是带色晶体，能溶于水。一般味苦。有些有剧毒。水解时生成糖和其他物质。和光提供多种生化用糖苷，HPLC级别标准品及食品分析专用试剂盒。可特异性切除赖氨酸C末端的多肽，用于Lys-X化合物的合成。
冻干粉，易溶于水或者缓冲液。
最佳pH值9.0~9.5，来源于细菌。
抑制剂是DFP，PMSF和TLCK。
水解底物包括 Tos-Lys-Ome,Bz-Lys-NH2.
