产品说明
This product is an unlabeled, affinity-purified chicken anti-C-MYC epitope antibody.Volume: 1 mLConcentration: 100 µg/mL (based on absorbance at 280 nm)Clonality: PolyclonalForm: Affinity PurifiedHost Species: ChickenApplications: ELISA, ICC, IHC, IP, WBAntibody Registry ID (RRID): AB_2313514Physical State: LiquidBuffer: Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) as an anti-microbial agent.Production Notes: Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin. The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a peptide column and then dialyzed against phosphate-buffered isotonic saline. Sodium azide was added and the antibodies were filter-sterilized (0.2 µm).Validation and Application NotesWestern Blot Dilution Range: 1:500-1:1000IHC Dilution Range: 1:100-1:250Quality ControlAntibodies were analyzed using ELISA and Western Blots using an N-terminal C-MYC-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.StorageStore at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.NOTEAves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.↑ back to top
神经元标志蛋白抗体
该系列产品主要用于神经元细胞的各亚细胞结构研究,可与现有的小鼠/兔抗体进行免疫双标,反应性包括人、小鼠等多个哺乳类动物。在保证抗体特异性的前提下,部分产品可分为抗不同的氨基酸片段。该系列产品包括抗APP3/APP345/APP4/APP5/ABN/TUJ/CAT/ DCX/ER1/ER2/ER3/ER5/GAD/GAP43/MAP/NET/ NUN/NSE/ NFH / NFL / NFM / PER/ PRN / PAP/ STG / TAU / TYH的抗体。除单独的抗体提供,我们还可同时提供1/3种任意抗体组合及相应二抗的试剂盒,以更优惠的价格及便捷性以满足研究人员的不同需要。
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