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DELLA protein RGL2
  • 货号:AS11 1803
    抗体名:兔抗拟南芥DELLA 蛋白 RGL2多克隆抗体
    抗体英文名:RGA-like protein 2|DELLA protein RGL2
    浓度:见说明书
    应用范围:western blot (WB)
    宿主:
    适应物种:植物
    标记物:
    克隆性:
    保存条件:低温
    亚型:IgG
    规格:200 µl

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    RGA-like protein 2|DELLA protein RGL2介绍:

    货   号:AS11 1803

    中文名称:兔抗拟南芥DELLA 蛋白 RGL2多克隆抗体

    英文名称:RGA-like protein 2|DELLA protein RGL2

    应用:western blot (WB)

    规格:200 µl

    价格:3975元

     

    RGA-like protein 2|DELLA protein RGL2简介:

    PRODUCT INFORMATION
    background 

    DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques.  Synonymes: GRAS family protein 15,AtGRAS-15, Scarecrow-like protein 19, AtSCL19.

    immunogen 

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt:Q8GXW1, TAIR: At3g03450

    host 

    rabbit

    clonality 

    polyclonal

    purity 

    affinity purified serum, in PBS pH 7.4

    format 

    lyophilized

    quantity 50 µg
    reconstitution For reconstitution add 50 µl of sterile water.
    storage 

    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    tested applications 

    western blot (WB)

    related products AS13 2638 | anti-GID2 | F-BOX protein GID2 (SLEEPY 1), rabbit antibodyAS12 1860 | anti-PIF4 | Phytochrome interacting factor 4, rabbit antibody
    additional information 

    to be added when available

    APPLICATION INFORMATION
    recommended dilution  

    1 : 1000 with standard ECL (WB) on transfected Arabidopsis thaliana protoplasts

    expected | apparent MW  

    60 kDa

    confirmed reactivity  

    Arabidopsis thaliana

    not reactive in  

    Triticum sp.

    additional information  

    As this protein is expressed in tissue in very low levels, please load at least 50 µg/well. Recommended extraction and denaturation protocol: 

    Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade. 
    selected references  

    to be added when available, antibody released in June 2014.