Range: 0.391 ng/ml - 100 ng/ml Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine Detection method: Colorimetric Assay type: Competitive Reacts with: Mouse, Rat, Human, Baboon 产品概述 Corticosterone ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the quantitative measurement of Corticosterone levels in plasma, serum, urine, milk, saliva and cell culture supernatant.A Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Corticosterone is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate.Please note the concentrations of the kit components are lot-specific and the end user should always refer to the vial labelThe entire kit may be stored at -20 C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles. 1 x 80µl10X DiluentS Concentrate 1 x 20ml1X Biotinylated Corticosterone Protein (Lyophilized) 1 vial20X Wash Buffer Concentrate 1 x 30mlChromogen Substrate 1 x 7mlCorticosterone Microplate (12 x 8 well strips) 1 unitCorticosterone Standard 1 vialSealing Tapes 3 unitsStop Solution 1 x 11ml Corticosterone is the adrenal steroid, the major glucocorticoid. Glucocorticoid hormones are also known as corticosteroid hormones and are synthesized mainly in the adrenal cortex; however, more recently the enzymes involved in their synthesis have been found in a variety of cells and tissues, including the heart. The effects of these hormones are mediated via both cytoplasmic mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs), which act as ligand-inducible transcription factor. Corticosterone has profound effect on the structure and function of the hippocampus. Brain corticosterone action through the glucocorticoid receptor may involve memory storage. Emotional stress might cause increases in plasma corticosterone. Corticosterone measured in biological fluids showing quantity (ng) per mL of tested sample. 发表研究结果有使用 ab108821？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab108821 被引用在 106 文献中. Xu XR et al. Activation of dopaminergic VTA inputs to the mPFC ameliorates chronic stress-induced breast tumor progression. CNS Neurosci Ther 27:206-219 (2021).PubMed: 33112032 Sun JS et al. Ventromedial hypothalamic primary cilia control energy and skeletal homeostasis. J Clin Invest 131:N/A (2021).PubMed: 33021968 Agrimi J et al. Psychosocial Stress Hastens Disease Progression and Sudden Death in Mice with Arrhythmogenic Cardiomyopathy. J Clin Med 9:N/A (2020).PubMed: 33255451 Alshammari TK et al. Examining the central effects of chronic stressful social isolation on rats. Biomed Rep 13:56 (2020).PubMed: 33123370 Ahmad H et al. Kaempferol Facilitated Extinction Learning in Contextual Fear Conditioned Rats via Inhibition of Fatty-Acid Amide Hydrolase. Molecules 25:N/A (2020).PubMed: 33066366 View all Publications for this product I used this product to measure levels of corticosterone in common frog (Rana temporaria) urine. I first tested two dilutions of the urine against the lowest and highest standard, and following this I tested each urine sample neat since levels of corticosterone were low. I didn\'t find the kit particularly easy to use, there are a lot of steps to follow and it is quite time confusing. The standard curve was accurate however. The majority of the frog urine samples were below the level of detection of the assay. J’ai effectué une extraction du corticostérone de fèces provenant de babouins Papio Anubis selon le protocole décrit dans l’article suivant « Monkeys in the Middle : Parasite Transmission through the Social Network of a Wild Primate » , Andrew J.J. Maclntosh,2012.(J’ai cuis les fèces dans un four à 60° pendant 3h pour 1 g de fèces).Ensuite j’ai utilisé le kit ab108821 de abcam pour doser la corticostérone.J’ai testé différentes dilutions et il en sort qu’on peut utiliser l’échantillon non dilué.J’ai obtenu des résultats qui correspondent au moyenne à une concentration de 4.52 ng/ml.Par contre au niveau des contrôles j’ai obtenu des résultats que je ne comprends pas :-Dans le premier contrôle je mets tout saut l’Ag et j’obtiens un très forte Abs, ce qui correspond à une très faible concentration – c’est normal.-Dans le 2ème contrôle je mets tout sauf le 2ème AcI (l’Ag utilisé est celui correspondant au point 1 de la courbe standard [100ng/ml]) et là j’obtient une Abs proche d’une Abs correspondant à la concentration [100ng/ml]).-Dans un 3ème contrôle je mets tout sauf l’AcII (l’Ag utilisé est celui correspondant au point 1 de la courbe standard [100ng/ml]) et là j’obtiens une Abs très faible correspondant à une concentration très forte.-Dans un 4ème contrôle je mets uniquement du diluant et là j’obtiens une Abs très faible correspondant à une concentration très forte comme dans le contrôle précédent.Corticosterone was extracted from feces of Papio Anubis baboons according to the protocol described in the following article : « Monkeys in the Middle : Parasite Transmission through the Social Network of a Wild Primate » , Andrew J.J. Maclntosh,2012.(I cook feces in an oven at 60 ° for 3 h per 1 g of feces).Then I used the kit ab108821 from Abcam to quantify the corticosterone.I tested different dilutions and found out undiluted sample can be used.I determined an average concentration of 4.52 ng/ml.However in the controls I obtained results I do not understand:- In the first test I put all jump Ag and I get a very strong Abs, which corresponds to a very low concentration - this is normal.- In the second control I put everything except the second ACI (Ag used is the one corresponding to the point of the standard curve [100ng/ml]) and then I get a close Abs Abs corresponding to a concentration [100ng/ml]).- In a third control I put everything except the ACII (Ag used is the one corresponding to the point of the standard curve [100ng/ml]) and then I get a very low Abs corresponding to a very high concentration.- In a fourth-control I put only the diluent and then I get a very low Abs corresponding to a high concentration as in the previous test. Unfortunately the components of this buffer are considered proprietary. And while this buffer has not been tested for use with protein assays, its likley that some of the components would cause erroneous results in a protein assay. Read More It is always good idea to run creatinine assay as a normalization factor for the urine sample since the urine concentration is varied significantly. Some of our customers do use a creatinine assay but others don\'t so it is really up to you. Read More Yes. This kit is a competitive ELISA assay. Corticostone is competing with the biotinylated-corticosterone. The higher the concentration of corticosterone in sample, the lower OD. Read More Thank you for contacting us.According to the protocol on our datasheet, this kit contains sealing tapes: Each kit contains 3 pre-cut, pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay. Regarding the storage, the protocol states the following: ab108821 may be stored until the expiration date at 2 to 8 C or -20 C from the date of shipment. Store SP Conjugate at -20 C upon arrival up to the expiration date. Store Microplate, Diluent Concentrate (10x), Wash Buffer, Stop Solution, and Chromogen Substrate at 2-8 C. Diluent (1x) may be stored for up to 1 month at 2-8 C. Store Standard and Biotinylated Protein at 2-8 C before reconstituting with Diluent and at -20 C after reconstituting with Diluent. Opened unused microplate wells may be returned to the foil pouch with the desiccant packs. Reseal along zip-seal. May be stored for up to 1 month in a vacuum desiccator.I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Read More Thank you for contacting us. Since Corticosterone is stable molecule, we think it should work. But there is no guarantee since we have not tested this condition.I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Read More La source du kit m\'a inform qu\'ils n\'avaient malheureusement jamais test ce genre de kits sur des selles. Leur recommandation est d\'essayer une s rie de dilutions au 1:2, 1:4, 1:8, etc.J\'esp re que ces informations vous seront utiles. N\'h sitez pas nous contacter de nouveau si vous avez d\'autres questions. Read More Code de remise : ****Date d expiration : jj mm aaaaJe suis ravi que vous souhaitiez participer notre offre et tester ab108821 sur selles de singe.Le code de r duction correspondant est ****. Ce code de r duction est utilisable sous 120 jours et pour l achat d un autre produit abcam. Inutile de mentionner ce code sur votre premi re commande.Pour activer ce code, vous devez dans un premier temps soumettre une Abreview avec vos r sultats chez le singe et noter ce code dans la section Additional notes . Ainsi activ , ce code vous donnera une r duction sur votre prochaine commande de la valeur du ab108821. Veuillez contacter notre service client le en vous munissant des informations suivantes : code de r duction, num ro de commande de ab108821.Je vais demander au laboratoire si ils ont des recommandations pour la pr paration des chantillons. Je vous contacterai de nouveau quand j\'aurai re u une r ponse. Merci pour votre patience. Read More Thank you for contacting us. Unfortunately, this kit has not been tested for cross-reactivity with 11-DHC, so we do not have any information about whether or not it would cross-react. I am sorry I could not be more helpful, please let me know if you have any additional questions. Read MorePlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com