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plastocyanin
  • 货号:AS06 141
    抗体名:兔抗质体蓝素多克隆抗体
    抗体英文名:PC|plastocyanin
    浓度:见说明书
    应用范围:western blot (WB), immunogold (IG)
    宿主:
    适应物种:植物
    标记物:
    克隆性:
    保存条件:低温
    亚型:IgG
    规格:200 µl

    供应商:上海经科化学科技有限公司 

     

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    PC|plastocyanin介绍:

    货   号:AS06 141

    中文名称:兔抗质体蓝素多克隆抗体

    英文名称:PC|plastocyanin

    应用:western blot (WB), immunogold (IG)

    规格:200 µl

    价格:3975元

     

    PC|plastocyanin简介:

    PRODUCT INFORMATION
    background 

    Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.

    immunogen 

    purified native plastocyanin from Spinacia oleracea P00289

    host 

    rabbit

    clonality 

    polyclonal

    purity 

    serum

    format 

    lyophilized

    quantity 50 µl
    reconstitution For reconstitution add 50 µl of sterile water.
    storage 

    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    tested applications 

    western blot (WB), immunogold (IG)

    related products 

    AS06 202 | anti-cytc6 marker antibody of thylakoid lumen for Chlamydomonas reinhardtii

    additional information 

    cellular [compartment marker] of chloroplast thylakoid lumen

    APPLICATION INFORMATION
    recommended dilution  

    1:2000 with alkaline phosphatase (WB), 1: 100 (IG)

    expected | apparent MW  

    10 kDa

    confirmed reactivity  

    Arabidops thaliana, Brassica juncea, Heliantus annuus, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Solanum tuberosum, Zea mays

    predicted reactivity  

    dicots Ricinus communis, Solanum lycopersicum, monocots including Hordeum vulgare, mossPhyscomitrella patens

    not reactive in  

    no confirmed exceptions from predicted reactivity known in the moment

    additional information  

    plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.

    PC1 runs closer to 14 kDa while PC2  runs closer to 19 kDa.  For good resolution adding fresh DTT to the sample buffer is recommended.  PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.

    selected references   Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492. Sook Seok et al. (2013). AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability. Physiologia Plantarum, DOI: 10.1111/ppl.12116. Perera-Garcia et al. (2013). Arabidopsis copper transport protein COPT2 participates in the crosstalk between iron deficiency responses and low phosphate signaling.

     

     

    application example 

     

    \"western

    10 µg of total protein from (1)Arabidopsis thaliana, (2)Brassica juncea, (3) Zea mays, (4) Oryza sativa, (5) Solamum lycopersicum, (6) Nicotiana tabacum, (7) Heliantus annuuswere separated on  SDS-PAGE and blotted to nitrocellulose. Filters were probed with anti-PC antibody  (AS06 141,1:2000). Signal was developed using alkaline phosphatase conjugated secondary antibody. Each sample was run in duplicate. Signal was developed using alkaline conjugated secondary antibody.