plastocyanin-蚂蚁淘生物

货号：	AS06 141
抗体名：	兔抗质体蓝素多克隆抗体
抗体英文名：	PC\|plastocyanin
浓度：	见说明书
应用范围：	western blot (WB), immunogold (IG)
宿主：	兔
适应物种：	植物
标记物：	无
克隆性：	否
保存条件：	低温
亚型：	IgG
规格：	200 µl

供应商：上海经科化学科技有限公司

服务热线：400-0199-638

QQ:472482400(上海经科）

微信号：shjkchem

活动：消费积分可换充值卡！

PC|plastocyanin介绍：

货号：AS06 141

中文名称：兔抗质体蓝素多克隆抗体

英文名称：PC|plastocyanin

应用：western blot (WB), immunogold (IG)

规格：200 µl

价格：3975元

PC|plastocyanin简介：

PRODUCT INFORMATION
background		Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.
immunogen		purified native plastocyanin from Spinacia oleracea P00289
host		rabbit
clonality		polyclonal
purity		serum
format		lyophilized
quantity		50 µl
reconstitution		For reconstitution add 50 µl of sterile water.
storage		store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications		western blot (WB), immunogold (IG)
related products		AS06 202 \| anti-cytc6 marker antibody of thylakoid lumen for Chlamydomonas reinhardtii
additional information		cellular [compartment marker] of chloroplast thylakoid lumen

APPLICATION INFORMATION
recommended dilution		1:2000 with alkaline phosphatase (WB), 1: 100 (IG)
expected \| apparent MW		10 kDa
confirmed reactivity		Arabidops thaliana, Brassica juncea, Heliantus annuus, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Solanum tuberosum, Zea mays
predicted reactivity		dicots Ricinus communis, Solanum lycopersicum, monocots including Hordeum vulgare, mossPhyscomitrella patens
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW. PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended. PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.
selected references		Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492. Sook Seok et al. (2013). AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability. Physiologia Plantarum, DOI: 10.1111/ppl.12116. Perera-Garcia et al. (2013). Arabidopsis copper transport protein COPT2 participates in the crosstalk between iron deficiency responses and low phosphate signaling.

application example

$\"western$

10 µg of total protein from (1)Arabidopsis thaliana, (2)Brassica juncea, (3) Zea mays, (4) Oryza sativa, (5) Solamum lycopersicum, (6) Nicotiana tabacum, (7) Heliantus annuuswere separated on SDS-PAGE and blotted to nitrocellulose. Filters were probed with anti-PC antibody (AS06 141,1:2000). Signal was developed using alkaline phosphatase conjugated secondary antibody. Each sample was run in duplicate. Signal was developed using alkaline conjugated secondary antibody.

友情链接