实验概要The following protocol describes a general procedure for subculturing adherent mammalian cells in culture.主要试剂1. Complete growth medium, pre-warmed to 37℃2. Balanced salt solution such as Dulbecco’s Phosphate Buffered Saline (DPBS), containing no calcium, magnesium, or phenol red3. Dissociation reagent such as trypsin or TrypLE™ Express, without phenol red主要设备1. Culture vessels containing adherent cells2. Disposa......阅读全文 Hemacytometer Workbook

This workbook was developed for use with Module 2 of theInVitro InsightsCell Culture Training Program, developed by Becton Dickinson. The

Fibroblast Cell Systems-4

3. If your result falls into any quadrant other than the High Yield-High Viability quadrant, refer to Appendix D, Improving Cell Yield and

细胞培养常规操作

常规操作(主要内容如下)·Aseptic Technique·Culture Ves

Fibroblast Cell Systems-3

SeedingAfter cells are thawed:NOTE:Do notdispense the entire contents of the cryovial into one T-25 flask!!Remove the cap, being car

Tissue Culture Methods-3

REFERENCES:R. Ian Freshney,Culture of Animal cells: A manual of basic techniques,Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEachs

Isolation of human primary ovarian surface epithelial cells

1.Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institutional Review (Refere

Fibroblast Cell Systems-6

APPENDIX EGROWTH AREA OF COMMON PLASTICWAREFlasksEffectiveGrowthAreaInitial Number of Cells to Seed at 3500 cells/cm2 NHDFExpected Number of Cel

Fibroblast Cell Systems-5

Hemacytometer Reference Figure 15.Determine the Cell Count.a. Calculate thetotalcells counted in the four corner squares.1) If theto

Isolation of human corneal endothelial cells (HCECs)

Isolation of HCECs1.The corneoscleral tissues were rinsed three times with primary cell culture system containing 50 mg/mL gentamicin and 1.

AMNIOTIC FLUID CULTURES ON COVERSLIPS

I. Purpose:Amniotic fluid may be used for prenatal diagnosis of aneuploidy or other structural abnormalities.II. Culture Procedure:A. Aseptic te

In Vitro Conservation and Cryopreservation of Ornamental Plants

Today, the conservation of ornamental germplasm can take advantage of innovative techniques which allow preservation in vitro (slow growth storage

AMNIOTIC FLUID CULTURES ON COVERSLIPS

实验概要AMNIOTIC FLUID CULTURES ON COVERSLIPS主要试剂Solutions:Colcemid working solution: 10 mcg/ml Colcemid in Hank s Balance

Cell Suspension Culture of Arabidopsis

实验概要Cell Suspension Culture of Arabidopsis主要试剂10% (v/v) Household BleachCallus Induction MediumGamborg s B5 Basal