product information Background Med 6 (Mediator of RNA polymerase II transcription subunit 6) is a component of the Mediator complex which functions in transmition of information from gene-specific reulatory proteins to the basal RNA polymerase II transcription machinery. Immunogen KLH-conjugated synthetic peptide derived from known higher plant Med6 sequences, including Arabidopsis thaliana UniProt: F4IXJ7,  TAIR: AT3G21350 Host Rabbit Clonality Polyclonal Clone Purity Affinity purified serum Format Lyophilized in PBS pH 7.4 Quantity 50 µg Reconstitution For reconstitution add 50 µl of sterile water. Storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications western blot (WB) Related products AS14 2803 | anti-Med 7a/b | Mediator of RNA polymerase II transcription subunit 7a/b, rabbit antibodiesAntibodies involved in RNA metabolism and transcription regulation. Plant and algal protein extraction buffer Secondary antibodies Additional information

application information
Recommended dilution
Expected | apparent MW		33.4 kDa
Confirmed reactivity		Arabidopsis thaliana
Predicted reactivity		please inquire
Not reactive in		no confirmed exceptions from predicted reactivity are currently known
Additional information
Selected references		to be added when available, antibody released in August 2015

application example 30 µg of total protein from Arabidopsis thaliana (leaves or seedlings tissues) extracted with extraction buffer (50 mM Tris/HCl pH7.5, 150mM NaCl, 5% glycerol, 1 mM EDTA, 5mM DTT, 0.5% (w/v) PVPP, 1% Protease inhibitors cocktail, 1% NP40) were separated on 10 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5% Milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (Goat anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 3 min. Courtesy of Dr. Ahmed Mohamed, School of Biological and Biomedical Sciences Durham University, United Kingdom

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