N-Succinimidyl N-[6-(Biotinamido)hexanoyl]-6-aminohexanoate (2mg 5) N-[6-(Biotinamido)hexanoyl]-6-aminohexanoic Acid N-Succinimidyl Ester (2mg 5) *Stock available in Belgium will be delivered in 1 to 3 days *Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments). Note: Product Information Volume/Quantity : 2 mg x 5 Molecular weight : 567.7 Description N-Hydroxysuccinimide (NHS) esters of biotin are commonly used as biotinylation reagents. B6096, Biotin-LC-LC-NHS reacts with the primary amines (-NH2) in buffers with pH between 7 and 9. The product is supplied in quantities suitable for biotinylation of up to 30 mg of antibodies. Storage This product should be stored at -20 ºC. Do not store at stock solution. Related Products B6097 Biotin-PEG2-NHS (2mg×5)H0586 HABA S0883 Sulfo-SMCC Sodium SaltH1621 Horseradish Peroxidase Maleimide Conjugate (0.5mg×3)B5944 BSA Maleimide Conjugate (1mg×3)T3531 Streptavidin Maleimide ConjugateS0951 Streptavidin from Streptomyces avidiniiS0966 Streptavidin FITC ConjugateS0972 Streptavidin HRP ConjugateS0993 Streptavidin DTBTA-Eu3 ConjugateDirections for Use Preparation: Use of a 10 mM Biotin-LC-LC-NHS solution is recommended. In order to efficiently biotinylate a sample, Biotin-LC-LC-NHS should be used at a 15-fold molar excess over the amount of amine-containing protein. Make sure to calculate the 10 mM biotin solution amount to be used with respect to the molar value of the sample (see example below). * Do not store reagent in solution. Calculate A uL of 10 mM biotinylation reagent solution : For 2mg IgG (150,000 M.W.) 2 [mg IgG] x 10-3 [g/mg] x 1/150,000 [mol/g] x 15 [fold]A [uL of 10 mM B6096 solution] x 10-6 [L/uL] x 10 [mmol/L] x 10-3 [mol/mmol] A 20 [uL of 10 mM B6096 solution] Procedure: 1. Bring Biotin-LC-LC-NHS (Product No. B6096) to room temperature. 2. Dissolve 2 mg of Biotin-LC-LC-NHS (Product No. B6096) in 350uL of dimethyl sulfoxide (DMSO) or dimethylformamide (DMF) and prepare a 10 mM solution of Biotin-LC-LC-NHS. 3. Dissolve the sample (1-10 mg/mL) in an amine-free buffer with pH between 7 and 9 such as PBS. Do not use buffers containing amines such as Tris. 4. Add A uL of 10 mM Biotin-LC-LC-NHS solution to the sample solution. 5. Incubate the mixed solution for 30 min at room temperature. 6. Remove unreacted and hydrolyzed reagent using desalting column or dialysis methods according to the manufacturer’s instructions. ReferencesD. Savage et al., in Avidin-Biotin Chemistry: A Handbook, 2nd ed., Pierce Chemical Company, 1992, pp. 42-43. Please Check that the Lot Number you have entered is correct.If your information is correct and you are still not able to view the requested certificates, PleaseContact Us for more information. +32(0)3 735 07 00 (Ireland +44(0) 1865 78 45 60; Switzerland Liechtenstein +49 6196 640 53 00)