Home »products » Cell Biology Product » 3D Tumor Culture Gel3D Cell Culture Gel - Col-Tgelfor tumor cell, stem cell and primary cellCol-Tgel Flyer Cat.#StiffnessVolumeStrength3 Stiffness ApplicationP720S-2Soft2 mL0.9 ~ 1.5 kPaCirculating cells, Nerve cells, BT474, HL60, P39P720S-10Soft10 mLP720M-2Med2 mL14 ~ 20 kPaMuscle cells, Adipose cells, C2C12, HepG2P720M-10Med10 mLP720H-2Stiff2 mL35 ~ 47 kPaBone cellsP720H-10Stiff10 mLP720SMH-6S/M/H2 mL x 3A combo of P720S-2, P720M-2, P720H-2 for customer testing their cells.Customer can mix any two gels at any ratio to make their own special stiffness gel..flatbtn {-webkit-box-sizing: border-box;-moz-box-sizing: border-box;box-sizing: border-box;display: inline-block;outline: 0;border: 0;color: #f3faef;text-decoration: none;background-color: #6bb642;border-color: rgba(0, 0, 0, 0.1) rgba(0, 0, 0, 0.1) rgba(0, 0, 0, 0.25);font-size: 1.2em;font-weight: bold;padding: 12px 22px 12px 22px;line-height: normal;text-align: center;vertical-align: middle;cursor: pointer;text-transform: uppercase;text-shadow: 0 1px 0 rgba(0,0,0,0.3);-webkit-border-radius: 3px;-moz-border-radius: 3px;border-radius: 3px;-webkit-box-shadow: 0 1px 0 rgba(15, 15, 15, 0.3);-moz-box-shadow: 0 1px 0 rgba(15, 15, 15, 0.3);box-shadow: 0 1px 0 rgba(15, 15, 15, 0.3);}.flatbtn:hover {color: #fff;background-color: #73c437;}.flatbtn:active {-webkit-box-shadow: inset 0 1px 5px rgba(0, 0, 0, 0.1);-moz-box-shadow:inset 0 1px 5px rgba(0, 0, 0, 0.1);box-shadow:inset 0 1px 5px rgba(0, 0, 0, 0.1);}/** custom login button **/.flatbtn-blu { -webkit-box-sizing: border-box;-moz-box-sizing: border-box;box-sizing: border-box;display: inline-block;outline: 0;border: 0;color: #edf4f9;text-decoration: none;background-color: #4f94cf;border-color: rgba(0, 0, 0, 0.1) rgba(0, 0, 0, 0.1) rgba(0, 0, 0, 0.25);font-size: 1.3em;font-weight: bold;padding: 12px 26px 12px 26px;line-height: normal;text-align: center;vertical-align: middle;cursor: pointer;text-transform: uppercase;text-shadow: 0 1px 0 rgba(0,0,0,0.3);-webkit-border-radius: 3px;-moz-border-radius: 3px;border-radius: 3px;-webkit-box-shadow: 0 1px 1px rgba(0, 0, 0, 0.2);-moz-box-shadow: 0 1px 1px rgba(0, 0, 0, 0.2);box-shadow: 0 1px 1px rgba(0, 0, 0, 0.2);}.flatbtn-blu:hover {color: #fff;background-color: #519dde;}.flatbtn-blu:active {-webkit-box-shadow: inset 0 1px 5px rgba(0, 0, 0, 0.1);-moz-box-shadow:inset 0 1px 5px rgba(0, 0, 0, 0.1);box-shadow:inset 0 1px 5px rgba(0, 0, 0, 0.1);}/** modal window styles **/#lean_overlay {position: fixed;z-index:100;top: 0px;left: 0px;height:100%;width:100%;background: #000;display: none;}#loginmodal {width: 800px;padding: 15px 20px;background: #fff;-webkit-border-radius: 6px;-moz-border-radius: 6px;border-radius: 6px;-webkit-box-shadow: 0 1px 5px rgba(0, 0, 0, 0.5);-moz-box-shadow: 0 1px 5px rgba(0, 0, 0, 0.5);box-shadow: 0 1px 5px rgba(0, 0, 0, 0.5);}#loginform { /* no default styles */ }#loginform label { display: block; font-size: 1.1em; font-weight: bold; color: #7c8291; margin-bottom: 3px; }.txtfield { display: block;width: 100%;padding: 6px 5px;margin-bottom: 15px;font-family: "Helvetica Neue", Helvetica, Verdana, sans-serif;color: #7988a3;font-size: 1.4em;text-shadow: 1px 1px 0 rgba(255, 255, 255, 0.8);background-color: #fff;background-image: -webkit-gradient(linear, left top, left bottom, from(#edf3f9), to(#fff));background-image: -webkit-linear-gradient(top, #edf3f9, #fff);background-image: -moz-linear-gradient(top, #edf3f9, #fff);background-image: -ms-linear-gradient(top, #edf3f9, #fff);background-image: -o-linear-gradient(top, #edf3f9, #fff);background-image: linear-gradient(top, #edf3f9, #fff);border: 1px solid;border-color: #abbce8 #c3cae0 #b9c8ef;-webkit-border-radius: 4px;-moz-border-radius: 4px;border-radius: 4px;-webkit-box-shadow: inset 0 1px 2px rgba(0, 0, 0, 0.25), 0 1px rgba(255, 255, 255, 0.4);-moz-box-shadow: inset 0 1px 2px rgba(0, 0, 0, 0.25), 0 1px rgba(255, 255, 255, 0.4);box-shadow: inset 0 1px 2px rgba(0, 0, 0, 0.25), 0 1px rgba(255, 255, 255, 0.4);-webkit-transition: all 0.25s linear;-moz-transition: all 0.25s linear;transition: all 0.25s linear;}.txtfield:focus {outline: none;color: #525864;border-color: #84c0ee;-webkit-box-shadow: inset 0 1px 2px rgba(0, 0, 0, 0.15), 0 0 7px #96c7ec;-moz-box-shadow: inset 0 1px 2px rgba(0, 0, 0, 0.15), 0 0 7px #96c7ec;box-shadow: inset 0 1px 2px rgba(0, 0, 0, 0.15), 0 0 7px #96c7ec;}#page-wrap {width: 760px;background: white;margin: 20px auto;padding: 0px 20px 20px 20px;}#page-wrap .button {float: left;margin: 10px;}#home {display: block;padding: 10px;}#home-button {opacity: 1.0;border-bottom: 1px solid black;padding:10px 20px;cursor: pointer;text-transform: uppercase;font-weight: bold;text-shadow: 0 1px 0 rgba(0,0,0,0.3);}#about {display: none;padding: 10px;}#about-button {opacity: 0.5;border-bottom: 1px solid black;padding:10px 20px;cursor: pointer;text-transform: uppercase;font-weight: bold;text-shadow: 0 1px 0 rgba(0,0,0,0.3);}#contact {display: none;padding: 10px;}#contact-button {opacity: 0.5;border-bottom: 1px solid black;padding:10px 20px;cursor: pointer;text-transform: uppercase;font-weight: bold;text-shadow: 0 1px 0 rgba(0,0,0,0.3);}.clear {clear:both;}#home img, #about img, #contact img{width:100%;}#pay_div a {text-decoration: none;}#pay_div a:hover{text-decoration: none;}How to pay with Step 1Step 2Step 1Step 2Also can buy from :P720SMH-6, Fisher Cat.# NC1291700ApplicationCol-Tgel is a tailorable 3D cell culture matrix for tumor cell, stem cell and primary cell. We provide soft, medium and stiff gel for your different cell types.It provides collagen backbone for cell attachment and allows customizing matrix components by additional extracellular matrix, growth factors, cytokines, and/or hormones for optimizing three dimensional cell culture.This product is for research use only. Stiffness selection for different cell typesClick to see full size image.Formation of tumor spheroids in Col-TgelFang, JY., etc. PloS one 9, no. 8 (2014): e105616. For immunohistochemistry and immunocytochemistry method, please refer to Page 2 of this publication.Unique Features Handle at room temperature (no need on ice)Save money: 30% below market priceSave time: No hanging drop, rotating wall vessel bioreactors3 stiffness(unique feature): to fit most cell types2 mL aliquotes: avoid freeze-thaw cycle - user friendlyCytokine / Hormon free: no backgroundFlexible: -Col-Tgel can be customized for different experiments.- Geometry: 3D embedding, 2D seeding on the top of hydrogel, or unique molds. -Sample size and quantity: 6- to 96- well with/without tissue culture insert or transwell. - Matrix component and stiffness: collagen-based with different yield strength (i.e. Soft, Med and Stiff). - Transparency: easy to observe directly under microscopes and can apply with immunohistochemistry directly. Col-Tgel is a tailorable collagen-based, cell remodelable hydrogel system. It not only can be used a carrier for cell or drug delivery, but also in vitro cell culture platforms. Besides, it contains cell essential nutrients, and provides a stable and durable platform for long-term studies such as cell-cell, cell-matrix interactions, and etc.Shipping / StorageShip at room temperature.Store Component A (gel) at 4oC, and store Component B (crosslinker) at -80oC for 9 months shelf life, or at -20oC for 3 months, or at 4oC for 1 month.Avoid freeze-thaw cycle for Component B.Shelf LifeComponent A: 12 months at 4oCComponent B: 9 months at -80oC; 3 months at -20oC; 1 month at 4oCManual (protocol)101Bio.com 3D Cell Culture Gel - Col-TgelCitationsCitation 1: Kuwahara, Kenrick, Zhi Yang, Ginger C. Slack, Marcel E. Nimni and Bo Han.Cell delivery using an injectable and adhesive transglutaminase–gelatin gel.Tissue Engineering Part C: Methods 16, no. 4 (2009): 609-618. Citation 2: Kuwahara, Kenrick, Josephine Y. Fang, Zhi Yang and Bo Han.Enzymatic crosslinking and degradation of gelatin as a switch for bone morphogenetic protein-2 activity. Tissue Engineering Part A 17, no. 23-24 (2011): 2955-2964. Citation 3: Fang, Josephine, Zhi Yang, ShihJye Tan, Charisse Tayag, Marcel E. Nimni, Mark Urata and Bo Han.Injectable gel graft for bone defect repair.Regenerative medicine 9, no. 1 (2014): 41-51. Citation 4: Tan, ShihJye, Josephine Y. Fang, Zhi Yang, Marcel E. Nimni and Bo Han. The synergetic effect of hydrogel stiffness and growth factor on osteogenic differentiation.Biomaterials 35, no. 20 (2014): 5294-5306. Citation 5: Fang, Josephine Y., Shih-Jye Tan, Zhi Yang, Charisse Tayag and Bo Han. Tumor Bioengineering Using a Transglutaminase Crosslinked Hydrogel.PloS one 9, no. 8 (2014): e105616. Citation 6: Shih-Jye Tan, Fang, Josephine Y., Y. Wu, Zhi Yang, G. Liang and Bo Han.Muscle tissue engineering and regeneration through epigenetic reprogramming and scaffold manipulation.Sci Rep. 2015 Nov 9;5:16333. doi: 10.1038/srep16333. Citation 7: Lee G, Kim HJ, Kim HM.RhoA-JNK Regulates the E-Cadherin Junctions of Human Gingival Epithelial Cells.J Dent Res. 2015 Dec 3. pii: 0022034515619375. [Epub ahead of print] Citation 8: Fang JY, Tan SJ, Wu YC, Yang Z, Hoang BX, Han B.From competency to dormancy: a 3D model to study cancer cells and drug responsiveness.J Transl Med. 2016 Feb 4;14(1):38. doi: 10.1186/s12967-016-0798-8. * Different names (TGase gel, TG gel) were used in old publications.They referred to the same 3D cell culture gel, and we finally name it as Col-Tgel.See all 101Bio"s citation papers, click here Tumor and regular tissue progression and development in Col-Tgel for in vitro and in vivo application.ul.tmo_list li img{margin-left:5px;margin-right:5px;}#content_wrapper .column_02 table {border: 0;}#content_wrapper .column_02table td{border: 0;padding: 10px;}#content_wrapper .column_02table tr.even{background: #eee;}Exosome IsolationPurity > 95%Protein Extraction1 min total protein, 40 min membrane protein3D Cell Culture Gel30% < mkt="">PCR Kits50% < mkt="">Mouse Brain Microvessel Isolation KitEasy protocol and high yieldEndotoxin-Free Plasmid Kitsmaxi, midi and mini-prep