Home »products » Molecular Biology Products » PCR Products » 2x Es Taq Master Mix (with dye)2x Es Taq Master Mix (with dye)Name2x Es Taq Master Mix (with dye)Cat. #W0690-1$ 39.00 (1 mL, 100 rxn, 20 ul/rxn)W0690-5$ 99.00 (5 mL, 500 rxn, 20 ul/rxn).flatbtn {-webkit-box-sizing: border-box;-moz-box-sizing: border-box;box-sizing: border-box;display: inline-block;outline: 0;border: 0;color: #f3faef;text-decoration: none;background-color: #6bb642;border-color: rgba(0, 0, 0, 0.1) rgba(0, 0, 0, 0.1) rgba(0, 0, 0, 0.25);font-size: 1.2em;font-weight: bold;padding: 12px 22px 12px 22px;line-height: normal;text-align: center;vertical-align: middle;cursor: pointer;text-transform: uppercase;text-shadow: 0 1px 0 rgba(0,0,0,0.3);-webkit-border-radius: 3px;-moz-border-radius: 3px;border-radius: 3px;-webkit-box-shadow: 0 1px 0 rgba(15, 15, 15, 0.3);-moz-box-shadow: 0 1px 0 rgba(15, 15, 15, 0.3);box-shadow: 0 1px 0 rgba(15, 15, 15, 0.3);}.flatbtn:hover {color: #fff;background-color: #73c437;}.flatbtn:active {-webkit-box-shadow: inset 0 1px 5px rgba(0, 0, 0, 0.1);-moz-box-shadow:inset 0 1px 5px rgba(0, 0, 0, 0.1);box-shadow:inset 0 1px 5px rgba(0, 0, 0, 0.1);}/** custom login button **/.flatbtn-blu { -webkit-box-sizing: border-box;-moz-box-sizing: border-box;box-sizing: border-box;display: inline-block;outline: 0;border: 0;color: #edf4f9;text-decoration: none;background-color: #4f94cf;border-color: rgba(0, 0, 0, 0.1) rgba(0, 0, 0, 0.1) rgba(0, 0, 0, 0.25);font-size: 1.3em;font-weight: bold;padding: 12px 26px 12px 26px;line-height: normal;text-align: center;vertical-align: middle;cursor: pointer;text-transform: uppercase;text-shadow: 0 1px 0 rgba(0,0,0,0.3);-webkit-border-radius: 3px;-moz-border-radius: 3px;border-radius: 3px;-webkit-box-shadow: 0 1px 1px rgba(0, 0, 0, 0.2);-moz-box-shadow: 0 1px 1px rgba(0, 0, 0, 0.2);box-shadow: 0 1px 1px rgba(0, 0, 0, 0.2);}.flatbtn-blu:hover {color: #fff;background-color: #519dde;}.flatbtn-blu:active {-webkit-box-shadow: inset 0 1px 5px rgba(0, 0, 0, 0.1);-moz-box-shadow:inset 0 1px 5px rgba(0, 0, 0, 0.1);box-shadow:inset 0 1px 5px rgba(0, 0, 0, 0.1);}/** modal window styles **/#lean_overlay {position: fixed;z-index:100;top: 0px;left: 0px;height:100%;width:100%;background: #000;display: none;}#loginmodal {width: 800px;padding: 15px 20px;background: #fff;-webkit-border-radius: 6px;-moz-border-radius: 6px;border-radius: 6px;-webkit-box-shadow: 0 1px 5px rgba(0, 0, 0, 0.5);-moz-box-shadow: 0 1px 5px rgba(0, 0, 0, 0.5);box-shadow: 0 1px 5px rgba(0, 0, 0, 0.5);}#loginform { /* no default styles */ }#loginform label { display: block; 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SpecificationsPolymerase:Es TaqHot Start:N/AFidelity:High fidelityGC-Rich PCR Performance:HighAmplification Range:6 kbExonuclease Activity:5´→3´Reaction Speed:1 kb / 30 secondsReaction Format:Master MixProduct Overhang:3"-ADescription and features This Master Mix contains Es Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer.The concentration is 2x.Es Taq DNA Polymerase possesses 5´→3´ DNA polymerase and 5´→3´ exonuclease activity. The polymerase has the high amplification efficiency and low mismatching as Taq DNA polymerase and high fidelity of Pfu DNA polymerase. Es Taq Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for T/A cloning.The amplification range of Es Taq is ~ 6 kb.This unique Master Mix recipe makes the system very reliable.More than 98% of PCR reaction can get successful amplification during the first try.It also works well on complicate templates.The Master Mix contains dye, and can directly run electrophoresis after PCR reaction.Shipping / StorageShip at 4℃. Store at -20℃ for up to 1 year and avoid freeze-thaw cycles. Stored at 4℃ for up to 3 months.Quality controlThis product is tested for no exogenous nuclease activity;no host DNA contamination tested (by PCR);able to amplify single copy gene from multiple genomes; and no significant enzyme activity decrease after storing at 2 ~ 8oC for 3 months.Manual (protocol)101Bio.com 2x Es Taq Master MixComponentsComponentsAmountW0690-1W0690-52x Es Taq Master Mix1 mL1 mL x 5RNase-Free Water1 mL5 mLPCR reaction systemNote: The recommended primer concentration for PCR is between 0.1-1.0 µM of each primer. The use of higher concentrations of primers can have higher amplification effect. Low primer concentration will generally ensure cleaner product and lower background.PCR reaction conditionsNote:The recommended annealing temperature is about 5℃ below Tm of primers. If extra bands are observed, higher annealing temperatures should be considered. The absence of product indicates the need for a lower annealing temperature.PCR extension time is depended on the size of target gene sequence.Es Taq DNA polymerase is approximately 1 kb DNA / 30 seconds.The number of PCR cycles will basically depend on the downstream application of the PCR product.PCR result examinationThis Master Mix contains dye for electrophoresis.After PCR, directly load 5 µL of PCR product to agarose gel to run electrophoresis.No need to add loading buffer. ul.tmo_list li img{margin-left:5px;margin-right:5px;}#content_wrapper .column_02 table {border: 0;}#content_wrapper .column_02table td{border: 0;padding: 10px;}#content_wrapper .column_02table tr.even{background: #eee;}Exosome IsolationPurity > 95%Protein Extraction1 min total protein, 40 min membrane protein3D Cell Culture Gel30% < mkt="">PCR Kits50% < mkt="">Beta-Hexosaminidase Activity Colorimetric Assay Fast and sensitive, High-throughputEndotoxin-Free Plasmid Kitsmaxi, midi and mini-prep