DescriptionProduct InfoApplication NoteReview   (0) Amount :Non Profit PD-1 Stable Cell Line is a stably transfected CHO-K1 cell line which expresses human Programmed Cell Death Protein -1 (PD-1, also known as CD279).Sequence data: hPD-1 (accession numberNP_005009)MQIPQAPWPVVWAVLQLGWRPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQTLVVGVVGGLLGSLVLLVWVLAVICSRAARGTIGARRTGQPLKEDPSAVPVFSVDYGELDFQWREKTPEPPVPCVPEQTEYATIVFPSGMGTSSPARRGSADGPRSAQPLRPEDGHCSWPL Content :Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO Storage condition :Immediately upon receipt, store in liquid nitrogen. Application:.Screen for antibodies of human PD-1 through Flow Cytometry.Culture conditions:Cells should be grown at 37oC with 5%CO2using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS supplemented with 10% FBS and 1% Pen/Strep, plus 10µg/ml of Blasticidin. (Note: Do not add Blasticidin to the parental CHO-K1 (Part #14500CCL) cell culture!)It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a37oCwater-bath, transfer to a tube containing 10 ml of growth medium without Blasticidin, spin down cells, resuspend cells in pre-warmed growth medium without Blasticidin, transfer resuspended cells to T25 flask and culture in37oC-CO2incubator.Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation.At first passage, switch to growth medium containing Blasticidin. Cells should be split before they reach complete confluence.To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly.To achieve satisfactory results, cells should not be passaged over 16 times.LIMITED USE RESTRICTIONS:THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.By use of this product, user agrees to be bound by the terms of this limited use statement.This product issolely for Internal Research Purposesandnot for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research.The buyer cannot sell, give or otherwise transfer this product to a third party.Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com). For Research Use Only. Not for use in diagnostic/therapeutics procedures. There are currently no product reviewsWrite a review on this product! Customers who purchased this product also purchasedGATA3 Leeporter™ Luciferase ...TNF-beta Leeporter™ Lucifera...TNF-beta Leeporter™ Lucifera...TNF-alpha Leeporter™ Lucifer...TNF-alpha Leeporter™ Lucifer...MIP-2 Leeporter™ Luciferase ...MIP-2 Leeporter™ Luciferase ...AP-1 Leeporter™ Luciferase R...IL-8 Leeporter™ Luciferase R...NF-kB Leeporter™ Luciferase ...Human Peripheral Blood CD19/CD...Human Peripheral Blood CD19 B ...Human Peripheral Blood CD14 Mo...Human Peripheral Blood CD8 Cyt... Most viewed ProductsRecombinant Human Thioredoxin-Like ...Monoclonal antibody to Human PD-L1 ...Monoclonal Antibody to Caspase-3 (P...Polyclonal Antibody to Beta actinMonoclonal Antibody to GAPDH (Clone...Monoclonal Antibody to Human IL-1be...Monoclonal antibody to B7-H4 (Clone...Polyclonal Antibody to Beta TubulinPeroxidase conjugated Goat anti Mou...Monoclonal Antibody to EpCAM (CD326...