产品说明
DescriptionReviews (0)DescriptionApplicationsL-luciferin is a potent competitive inhibitor of firefly luciferase.General InformationThe BioThema L-Luciferin is produced with a unique synthesis that includes special purification method which removes side-products from earlier steps in the synthesis. This method results in a top scoring biochemical activity according to the presentation “comparison of purity and activity of D-Luciferin from 8 different manufactors” published in Luminescence, Vol 27 Issue 2 and presented at the International Symposium on Bioluminescence and Chemiluminescence (ISBC). The high emission of our D-Luciferin means a lower detection limit which sometimes, though falsely, is referred to as sensitivity. The end-product is vacuum-dried and stored under argon to ensure maximum stability. The product is available in 100 mg. Other amounts can be arranged provided a longer delivery time is accepted.The BioThema Luciferin commes in four formes:L-Luciferin Free AcidD-Luciferin Free AcidD-Luciferin Potassium saltD-Luciferin Sodium saltDissolving L-LuciferinL-luciferin (acid form) can be dissolved at a concentration of 10 mg/mL (35.7 mmol/L) in an equimolar concentration of base, e. g. Tris(hydroxymethyl)aminomethane (Tris base) or sodium/potassium bicarbonate. It may take up to 30 min to dissolve the last few crystals.Stability of L-luciferinL-luciferin is sensitive to light and oxygen, before as well as after dissolving. If the vial containing the solid L-luciferin must be opened and closed again, it is recommended to fill the vial with an inert gas before closing the lid. Argon has a higher density than air. This makes it more convenient to fill the vial with argon as compared to nitrogen. The solid material should be stored at -20 °C. Solutions of D-luciferin should be protected from light and stored at -80 °C.Product characteristicsDelivery form: Single reagentNumber of assays: Depending on application. For further guidence please contact us at info@biothema.com.Delivery form: 100 mg in a glass vial.Storage: -20 °CShelf life: 5 years from date of delivery at -20 °C.ReviewsThere are no reviews yet.Be the first to review “L-Luciferin Free Acid 100 mg” Cancel replyYou must be logged in to post a review.
BioThema完全专注于萤火虫荧光素酶反应的分析应用,无论是检测细胞,测量酶活性还是其他。在该反应中,萤光素被ATP(三磷酸腺苷)活化,然后被氧化成氧萤光素和CO 2。氧化荧光素以激发态形成,并在进入基态时发光。通常在所谓的发光计中测量光。
ATP +萤光素+ O 2-萤光素酶-> AMP +焦磷酸盐+氧化萤光素+ CO 2 +光
在ATP的测定中,光发射可以基本恒定或迅速衰减。因此,存在基于萤火虫荧光素酶反应的稳定的光和闪光型ATP试剂。在萤光素酶含量低的情况下,每分钟消耗的ATP比例可忽略不计,因此光几乎可以保持恒定,但也很低。在荧光素酶水平高的情况下,不可避免地会消耗ATP,导致光线迅速减少,但最初的峰值光线很高。实际上,峰高和衰减率均与萤光素酶活性成正比
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