首页 > covalab > covalab/Beta-actin antibody (BA3R)/25 ug/mab90010-P

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covalab/Beta-actin antibody (BA3R)/25 ug/mab90010-P

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mab90010-P

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DatasheetProduct descriptionPublications & ImagesRelated productsProduct Codemab90010-PCloneBA3RImmunogenbeta-Actin N-terminal peptide-KLH conjugatesTarget Specificity Reacts with 42kDa native and denatured forms of beta-actin from human, mouse, rat, rabbit, and chicken.NameBeta-actinUniprot IDP60709Gene ID60Print datasheetProduct typePrimary antibodies, Loading controlsClonalityMonoclonal antibodyIsotypeIgG2bProduced inMouseSpeciesHuman, Mouse, Rat, Rabbit, ChickenLabellingNoneAppearanceLiquidConc. / Activity1mg/mlFormPurifiedConstituentsPBS 10mM, (pH 7.2)PreservativesNaN3 0.05%StorageShort term storage at +4°C (add 0.1% NaN3).For extended periods store in aliquots at -20°C.Antibodies are guaranteed for 6 month from date of receipt.ApplicationsWestern Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry, ELISA, ImmunoprecipitationWorking dilutionsOptimal dilution should be determined by the end user. The following are guidelines only :WB(1:1000 - 1:5000)FC(0.1 - 1µg for 106^ cells)ICC/IF(10 µg/ml)IP(5 µg/ml)Research areasCell metabolismPrint datasheet* Deygas M et al. Nat Commun. 2018 Oct 31;9(1):4545. (WB, Hu)(mab90010-P)Anti-beta-actin antibody WB staining of cell lysates from (1) human, (2) mouse, (3) rat, (4) rabbit."> (mab90010-P)Overlay histogram showing HeLa cells stained with mab90010-P (red line). Cells were fixed and permeabilized before staining. Unlabelled cells correspond to blue line and isotype control to black line."> (mab90010-P)Anti-beta-actin antibody IF staining (green) of HeLa cells. Plasma membranes are labelled in red and nuclei in blue (DAPI)."> (mab90010-P)Beta-actin IP using HeLa whole cell extract (5 µg of antibody for 0.5 mg of cell extract)">