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covalab/His-Tag antibody (HIS.H8 / EH158)/25 ug/mab90001-P
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DatasheetProduct descriptionPublications & ImagesRelated productsProduct Codemab90001-PCloneHIS.H8 / EH158ImmunogenHHHHHH (6x His) synthetic peptide conjugated to KLHTarget Specificity Reacts with the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag"s location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)Namepoly-histidinePrint datasheetProduct typePrimary antibodies, TagsClonalityMonoclonal antibodyIsotypeIgG2bProduced inMouseLabellingNoneAppearanceLiquidConc. / Activity1 mg/mLFormPurifiedConstituentsPBS 10mM, (pH 7.2)PreservativesNaN3 0.05%StorageShort term storage at +4°C (add 0.1% NaN3).For extended periods store in aliquots at -20°C.Antibodies are guaranteed for 6 month from date of receipt.ApplicationsWestern Blot, Immunocytochemistry, ELISA, ImmunoprecipitationWorking dilutionsOptimal dilution should be determined by the end user. The following are guidelines only : WB 1:1000 - 1:3000 ICC1:500 - 1:2000Print datasheet* Ossareh-Nazari B et al. Ubiquitylation by the Ltn1 E3 ligase protects 60S ribosomes from starvation-induced selective autophagy. J Cell Biol. 2014 Mar 17,204(6):909-17.(mab90001-P)Anti-His Tag antibody ICC staining of His-tag fusion protein in 293 cells.Left hand panel: untransfected controlRight hand panel: transfected.Counterstained with DAPI (blue)."> (mab90001-P)Anti-His Tag antibody WB staining of recombinant His-tagged Tgase-2 (1) 300 ng or (2) 30 ng. Antibody mab90001-P dilution 1/1000"> (mab90001-P)Anti-His Tag antibody ICC staining of transfected human HEK293 cells. DAPI staining (blue)."> (mab90001-P)Anti-His Tag antibody WB staining of various amounts of E. coli lysate containing a multi-epitope tagged protein">

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