ÜbersichtArtikelnummer711-095-152WirtsspeziesEselIgG-FormIgG GesamtmolekülSpezies-ReaktivitätKaninchenSpezifitätIgG (H+L)IsotypIgKlonalität (Mono-/Polyklonal)polyklonalAnwendungDurchflusszytometrie (Flow Cytometry), ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe)KonjugationFITC (Fluoresceinisothiocyanat)Maximale Anregung492 nmMaximale Emission520 nmKeine Kreuzreaktivität (MinX) mitHamster syrisch, Huhn, Human, Maus, Meerschweinchen, Pferd, Ratte, Rind, Schaf, ZiegeVerdünnungfür die meisten Anwendungen 1:50 – 1:200Format0,05% NaN3, 15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM PBS (pH 7,6), LyophilisatZweckbestimmungnur für ForschungszweckeProduktlinie / ThemaJackson ImmunoResearchHersteller / MarkeJackson ImmunoResearch Datenblätter711-095-152 – Datenblatt Weitere ProduktinformationenBased on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum proteins, but it may cross-react with immunoglobulins from other species.ConjugateFITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is still a widely used fluorophore due to its long history. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium. A better choice for many applications involving FITC is Alexa Fluor 488 because it is brighter and more photostable.