Supplementary Protocol for NP swabs, aspirates and BAL samples OverviewMag-Bind® Viral DNA/RNA Kit is designed for the rapid and reliable isolation of viral RNA and viral DNA from serum, swabs, plasma, saliva, and other body fluids. The Mag-Bind® magnetic beads technology enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. In addition to easily being adapted with automated systems, this procedure can also be scaled up or down depending on the amount of starting sample. The purified nucleic acids are ready for direct use in downstream applications such as amplification or other enzymatic reactions.Protocols are available for the following automated platforms:Hamilton Microlab® STARHamilton Microlab® NIMBUSKingFisher™, BioSprint®, and MagMAX® 96Adaptable to  other liquid handling platformsCoronavirus (SARS-CoV-2) ExtractionOmega Bio-tek is assisting scientists, researchers and healthcare workers around the globe in accelerating the screening and detection of the novel coronavirus disease, COVID-19. We are supporting several Laboratory Developed Tests (LDTs) by providing high-throughput, automated viral nucleic acid extraction methodologies. To meet the exceptional and immediate need for supplies, Omega Bio-tek has ramped up the production of its Mag-Bind® Viral DNA/RNA 96 kit and has the capacity to support 1 million patient tests per month.Supplement Protocol for NP swabs, aspirates and BAL samplesFully automatable and ready-to-load scripts for: KingFisher™:  Email product_support@omegabiotek.com for script Hamilton Microlab STAR:  384 samples in ~3 hr Hamilton MagEx STARlet:   96 samples in ~1 hr 45 minDedicated technical and application support to expedite setup and validation time.Contact a specialist SpecificationsFor Research Use Only. Not for use in diagnostic procedures.FeaturesSpecificationsStarting Amount50 µL - 200 µLStarting MaterialSerum, plasma, saliva and other body fluidsElution Volume20-50 μLTechnologyMagnetic BeadsProcessing ModeAutomated, ManualThroughput8 - 96Kit ComponentsItemAvailable SeparatelyMag-Bind® Particles CNRCall for PricingTNA Lysis Buffer---VHB BufferView ProductCarrier RNA---Proteinase K Solution (40 mg/mL)Call for PricingSPR Wash Buffer---Nuclease-free WaterView ProductProtocol and ResourcesProduct Documentation & LiteraturePROTOCOLM6246 Mag-Bind® Viral DNA/RNA 96 KitPROTOCOLM6246 Supplementary Protocol for NP Swabs, Aspirates and BAL SamplesSDSM6246 SDSSALES SHEETProduct Data#gap-1164158196{padding-top:30px}Mag-Bind ® Viral DNA/RNA Kit had better yield than a leading competing product Figure 1. HBV virus (in quantities of 10 and 1 infectious unit[s]) was spiked into 200 µL human serum. Viral nucleic acid was isolated with Omega Bio-tek’s Mag-Bind® Viral DNA/RNA Kit and with a comparable kit from Company A according to recommended protocols. 5 µL of template was used for a SYBR® Green-labeled qPCR reaction which was replicated 4 times. The resulting mean Ct values are shown in the above figure.#gap-117084170{padding-top:30px}DNA/RNA purified with Mag-Bind® Viral DNA/RNA had less inhibitor than with a leading competing product Figure 2. Nucleic acid was isolated from 200 µL of human whole blood with Omega Bio-tek’s Mag-Bind® Viral DNA/RNA Kit and a comparable kit from Company A using the manufacturer’s recommended protocols. The extractions were eluted in 100 µL. Three concentrations of template were used as templates in a SYBR® Green-labeled qPCR reaction. Each reaction was performed in quadruplicate and the mean Ct value is depicted in the above figure.#gap-909609470{padding-top:30px} Figure 3. 50 µL of ZeptoMetrix’s NATtrol Influenza A/B Positive Control standard was spiked into 150 µL of human serum and viral nucleic acids were extracted using Omega Bio-tek’s Mag-Bind® Viral DNA/RNA 96 Kit. Average Ct values obtained after amplification using Influenza B primers are shown above. The results indicate positive detection of Influenza B in both undiluted and 10-fold diluted purified samples.#gap-974094646{padding-top:30px}Table 1.  Some of the viruses* detected using our viral kits.Influenza AHepatitis ESheep pox virusInfluenza BInfectious Bronchitis virusMurine norovirus 1West Nile virusPorcine reproductive and respiratory syndrome Virus (PRRSV)Canine distemper virusMiddle East Respiratory Syndrome Coronavirus (MERS-CoV)Insect-specific flaviviruses, mononegaviruses, and totivirusesRabies virusZika virus (ZIKAV)orf virus (ORFV)RotavirusSIVPorcine circovirus type 2 (PCV2)Coxsackievirus B3HIVArbovirusesCoxsackievirus A6Influenza A (H1N1)Dengue virusAvian leukosis virus subgroup JHepatitis A virus types 1 and 3GB virus CAvian Encephalomyelitis VirusHepatitis B virusBovine Viral Diarrhea Virus (BVDV)Crimean-Congo hemorrhagic fever virus *References available upon requestPublicationsFauver, Joseph R., Shamima Akter, et al. “A Reverse-Transcription/RNase H Based Protocol for Depletion of Mosquito Ribosomal RNA Facilitates Viral Intrahost Evolution Analysis, Transcriptomics and Pathogen Discovery.” Virology, vol. 528, Feb. 2019, pp. 181–197, doi:10.1016/j.virol.2018.12.020.‌Gu, Shao-peng, et al. “Identification and Phylogenetic Analysis of the Sheep Pox Virus Shanxi Isolate.” Www.Biomedres.Info, 2018, www.biomedres.info/biomedical-research/identification-and-phylogenetic-analysis-of-the-sheep-pox-virus-shanxi-isolate-9658.html.‌Fauver, Joseph R., James Weger-Lucarelli, et al. “Xenosurveillance Reflects Traditional Sampling Techniques for the Identification of Human Pathogens: A Comparative Study in West Africa.” PLOS Neglected Tropical Diseases, edited by Paulo Filemon Pimenta, vol. 12, no. 3, Mar. 2018, p. e0006348, doi:10.1371/journal.pntd.0006348.‌Prakash, Ravi, et al. “Detection of Arboviruses in Blood and Mosquito Slurry Samples Using Polymer Microchip.” IEEE Xplore, 1 Nov. 2017, pp. 168–171, doi:10.1109/HIC.2017.8227611.‌Gloria-Soria, A., et al. “Infection Rate of Aedes Aegyptimosquitoes with Dengue Virus Depends on the Interaction between Temperature and Mosquito Genotype.” Proceedings of the Royal Society B: Biological Sciences, vol. 284, no. 1864, Oct. 2017, p. 20171506, doi:10.1098/rspb.2017.1506.#gap-642110227{padding-top:30px}View More PublicationsSpivey, Timothy J., et al. “Maintenance of Influenza A Viruses and Antibody Response in Mallards (Anas Platyrhynchos) Sampled during the Non-Breeding Season in Alaska.” PLOS ONE, edited by Balaji Manicassamy, vol. 12, no. 8, Aug. 2017, p. e0183505, doi:10.1371/journal.pone.0183505.‌Fauver, Joseph R., Brian D. Foy, et al. “The Use of Xenosurveillance to Detect Human Bacteria, Parasites, and Viruses in Mosquito Bloodmeals.” The American Journal of Tropical Medicine and Hygiene, vol. 97, no. 2, Aug. 2017, pp. 324–29, doi:10.4269/ajtmh.17-0063.‌Yu, Yongzhong, et al. “Characterization of an Orf Virus Isolate from an Outbreak in Heilongjiang Province, China.” Archives of Virology, vol. 162, no. 10, June 2017, pp. 3143–49, doi:10.1007/s00705-017-3426-x.‌Rückert, Claudia, et al. “Impact of Simultaneous Exposure to Arboviruses on Infection and Transmission by Aedes Aegypti Mosquitoes.” Nature Communications, vol. 8, May 2017, doi:10.1038/ncomms15412.Chotiwan, Nunya, et al. “Rapid and Specific Detection of Asian- and African-Lineage Zika Viruses.” Science Translational Medicine, vol. 9, no. 388, May 2017, doi:10.1126/scitranslmed.aag0538.‌Law, Jessica, et al. “Induction of Humoral Immune Response in Piglets after Perinatal or Post-Weaning Immunization against Porcine Circovirus Type-2 or Keyhole Limpet Hemocyanin.” Canadian Journal of Veterinary Research, vol. 81, no. 1, Jan. 2017, pp. 5–11, www.ncbi.nlm.nih.gov/pmc/articles/PMC5220598/.‌Grubaugh, Nathan D., et al. “Transmission Bottlenecks and RNAi Collectively Influence Tick-Borne Flavivirus Evolution.” Virus Evolution, vol. 2, no. 2, July 2016, doi:10.1093/ve/vew033.Puryear, Wendy Blay, et al. “Prevalence of Influenza A Virus in Live-Captured North Atlantic Gray Seals: A Possible Wild Reservoir.” Emerging Microbes & Infections, vol. 5, no. 1, Jan. 2016, pp. 1–9, doi:10.1038/emi.2016.77.Bliss, N., et al. “Prevalence of Influenza A Virus in Exhibition Swine during Arrival at Agricultural Fairs.” Zoonoses and Public Health, vol. 63, no. 6, Jan. 2016, pp. 477–85, doi:10.1111/zph.12252.‌McCorkell, Robert, et al. “Acute BVDV-2 Infection in Beef Calves Delays Humoral Responses to a Non-Infectious Antigen Challenge.” The Canadian Veterinary Journal, vol. 56, no. 10, Oct. 2015, pp. 1075–1083, www.ncbi.nlm.nih.gov/pmc/articles/PMC4572827/.‌Solis Worsfold, Cristina, et al. “Assessment of Neutralizing and Non-Neutralizing Antibody Responses against Porcine Circovirus 2 in Vaccinated and Non-Vaccinated Farmed Pigs.” Journal of General Virology, vol. 96, no. 9, Sept. 2015, pp. 2743–48, doi:10.1099/vir.0.000206.Eschbaumer, Michael, et al. “Probe-Free Real-Time Reverse Transcription Polymerase Chain Reaction Assays for the Detection and Typing of Porcine Reproductive and Respiratory Syndrome Virus in Canada.” Canadian Journal of Veterinary Research, vol. 79, no. 3, July 2015, pp. 170–179, www.ncbi.nlm.nih.gov/pmc/articles/PMC4445508/.Dow, Natalie, et al. “Genetic Variability of Bovine Viral Diarrhea Virus and Evidence for a Possible Genetic Bottleneck during Vertical Transmission in Persistently Infected Cattle.” PLOS ONE, edited by Binu T Velayudhan, vol. 10, no. 7, July 2015, p. e0131972, doi:10.1371/journal.pone.0131972.‌Yan, Mengfei, et al. “Infection of Porcine Circovirus 2 (PCV2) in Intestinal Porcine Epithelial Cell Line (IPEC-J2) and Interaction between PCV2 and IPEC-J2 Microfilaments.” Virology Journal, vol. 11, no. 1, Nov. 2014, doi:10.1186/s12985-014-0193-0.‌FormatMagnetic beads