OverviewThe Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is designed for isolation of high-quality genomic DNA from blood samples, saliva, swabs, mouse tails, dried blood spots or cultured cells. Mag-Bind® Particles HDQ provides quick magnetic response time reducing overall processing time. This system combines the reversible nucleic acid-binding properties of Mag-Bind® paramagnetic particles with Omega Bio-tek’s tissue and blood DNA isolation systems. Utilizing paramagnetic particles provides high-quality DNA that is suitable for direct use in most downstream applications such as Next Generation Sequencing, qPCR, PCR, and microarrays. The Mag-Bind Blood & Tissue DNA HDQ 96 Kit can be scaled up to process 1 mL whole blood samples. For larger volumes, the Mag-Bind® Blood DNA HV Kit can be used.#gap-1731426861{padding-top:30px}Safe – No organic solventsCompatible with DNA Genotek’s preservation systemCompatible with Mawi iSWAB DNA preservation systemHigh quality – Pure DNA suitable for downstream applications such as Next Generation Sequencing, qPCR, PCR, and microarrays Protocols are available for the following automated platforms:#gap-1307182660{padding-top:30px}Hamilton Microlab® STARHamilton Microlab® NIMBUSKingFisher™, BioSprint®, and MagMAX® 96SpecificationsFor Research Use Only. Not for use in diagnostic procedures.FeaturesSpecificationsStarting Amount100-250 μL blood samples, saliva, swabs, mouse tails, dried blood spots or 5 x 106 cultured cells.Elution Volume50-200 µLTechnologyMagnetic BeadsProcessing ModeAutomated, ManualThroughput8 - 384NoteDownstream applications: NGS, qPCR, microarrayKit ComponentsItemAvailable SeparatelyMag-Bind® Particles HDQCall for PricingAL BufferView ProductTL BufferView ProductHDQ Binding BufferView ProductVHB BufferView ProductSPM Wash BufferView ProductProteinase K SolutionView ProductElution BufferView ProductProtocol and ResourcesProduct Documentation & LiteraturePROTOCOLM6399 Mag-Bind® Blood & Tissue DNA HDQ 96 KitSDSM6399 SDSSALES SHEETVIDEO PROTOCOLAPPLICATION NOTEHigh Throughput, Automated DNA Extraction Solution from Whole Blood Samples Using Omega Bio-tek’s Reagents on Tecan Fluent® 780 WorkstationAPPLICATION NOTEDNA Extraction from Whole Blood on Hamilton’s Microlab® STAR™APPLICATION NOTEComprehensive, High Throughput Workflow for Automated gDNA Isolation from iSWAB Oral SamplesAPPLICATION NOTEA Simple, User-Friendly, High Throughput DNA Extraction Workflow Using 2-D Barcoded Buccal Swabs on Hamilton’s Firefly NIMBUS® 96APPLICATION NOTEFully automated DNA extraction solution from Omega Bio-tek using saliva stabilized in Biomatrica’s Salivagard® HT DNA collection tubesProduct Data#gap-916768706{padding-top:30px}DNA yield from 200 μL whole blood extracted using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit on the ABI MagMAX 96 instrument. Figure 1.  DNA yield from 200 µL whole blood extracted using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit on the ABI MagMAX 96 instrument. DNA yield was determined by PicoGreen® quantification.#gap-586553773{padding-top:30px}DNA yield from buccal swab samples using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is higher than a leading competitor. Figure 2.  Genomic DNA was extracted from buccal swab samples using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit and Company A’s genomic DNA extraction kit. 10% of DNA extracted from each sample was analyzed on a 0.8% agarose gel.#gap-1423154419{padding-top:30px}Purified genomic DNA using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is of high quality and free of inhibitors. Figure 3.  Genomic DNA was extracted from 1,500 µL whole blood using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit. The elution volume was 500 µL. DNA was analyzed on Thermo Scientific’s NanoDrop® 2000c. 50 ng of DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors.#gap-883891798{padding-top:30px}DNA purified using Mag-Bind Blood & Tissue DNA HDQ 96 Kit was around 60 kb Figure 4.  The sizes of genomic DNA purified using Mag-Bind Blood & Tissue DNA HDQ Kit were determined by electrophoresis.  Sample 1 and 2 were gDNA from human blood.  L is 50 kb genomic ladder for Agilent TapeStation 2200.  The size of gDNA is around 60 kb.#gap-2107885599{padding-top:30px}CitationsView CitationsV. Kilaru, S. V. Iyer, L. M. Almi, J. S. Stevens, A. Lori, T. Jovanovic, T. D. Ely, B. Bradley, E. B. Binder, N. Koen, D. J. Stein, K. N. Conneely, A. P. Wingo, A. K. Smith, K. J. Ressler. Genome-wide gene-based analysis suggests an association between Neuroligin 1 (NLGN1) and post-traumatic stress disorderXi Wang, Kai Wang, Wei Zhang, Ming Qiang, Ying Luo. A bilaminated decellularized scaffold for islet transportation: structure, properties and functions in diabetic micePavel Dimens. Population structure of a migratory small coastal shark, the blacknose shark Carcharhinus acronotus, across cryptic barriers to gene flowMolly Norton Darr. Biological studies and evaluation of Scymnus coniferarum crotch, a predator of hemlock woolly adelgid from western North AmericaSizeFREE SAMPLE, 1 x 96 Preps, 4 x 96 PrepsFormatMagnetic beads