OverviewThe E.Z.N.A.® Soil DNA Kit is formulated to isolate high purity cellular DNA from soil samples typically containing humic acid and other inhibitors of PCR. This kit uses a novel and proprietary method to isolate genomic DNA from a variety of environmental samples without organic extractions.This kit has been successfully used to isolate DNA from Gram-positive and -negative bacteria, fungi, yeast, and algae that inhabit a range of samples including clay, sandy, peaty, chalky, or loamy soil samples. Isolated DNA can be used for most downstream applications, including PCR, Southern blot, and NGS analysis.Reliable – Reproducible DNA purification from a variety of sample sourcesHigh quality – Ready-to-use DNA eliminating PCR inhibitors using proprietary inhibitor removal technologyYield – Efficient purification of DNA from even specialized samplesEase of use – Contains glass beads pre-filled in 2 mL vialsSpecificationsFor Research Use Only. Not for use in diagnostic procedures.FeaturesSpecificationsStarting AmountUp to 1 gStarting MaterialSoilYieldDependent upon sampleElution Volume 50-100 μLTechnology HiBind® DNA Mini ColumnProcessing ModeManualThroughput1-24Kit ComponentsItemAvailable SeparatelyHiBind® DNA Mini ColumnsView Product2 mL Collection TubesView ProductDisruptor TubesView ProductSLX-Mlus BufferView ProductDS BufferCall for PricingP2 BufferView ProductcHTR ReagentView ProductXP1 Buffer---HBC BufferView ProductDNA Wash BufferView ProductElution BufferView ProductProtocol and ResourcesProduct Documentation & LiteraturePROTOCOLD5625 Soil DNA KitSDSD5625 SDSQUICK GUIDED5625 Quick GuideSALES SHEETAPPLICATION NOTESuperior Performance of Omega Bio-tek’s E.Z.N.A.® Soil DNA Kit Over CompanyM’s Soil DNA Isolation Kit for DNA Extraction from Soil SamplesProduct Data#gap-210515131{padding-top:30px}DNA purified from soil samples using E.Z.N.A.® Soil DNA Kit has higher and more consistent yield than using a leading competing product. Figure 1. Comparison of DNA extraction method from soil samples. DNA yield determined with fluorescence-based dye quantification. 50 µL ZymoBIOMICS™ Microbial Community Standard was added to 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.#gap-275559726{padding-top:30px}DNA purified from soil samples using E.Z.N.A.® Soil DNA Kit has better PCR performance than using a leading competing product. Figure 2. Comparison of Ct values. 20 µL SYBR Green qPCR reaction. 50 µL ZymoBIOMICS™ Microbial Community Standard was added to 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.#gap-2074043327{padding-top:30px}E.Z.N.A.® Soil DNA Kit performs especially better for gram-positive bacteria than a leading competing product. Figure 3. DNA yield by bacterial classes. DNA yield determined with fluorescence-based dye quantification. 0.5 mL cultured Gram-positive and Gram-negative bacteria were added to corresponding 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.#gap-1016003901{padding-top:30px}PublicationsView PublicationsBao, Yun-Juan, et al. “High-Throughput Metagenomic Analysis of Petroleum-Contaminated Soil Microbiome Reveals the Versatility in Xenobiotic Aromatics Metabolism.” Journal of Environmental Sciences, vol. 56, 1 June 2017, pp. 25–35, www.sciencedirect.com/science/article/pii/S1001074216306155?casa_token=QXfnahukMoEAAAAA:H6xUoAHyfOBFs6fE1a0KcdKtiPZ53A_6EwwHMyW2uqsNhyydq52wc2CqS_UZCLFTCMB3hrpJ4yk, 10.1016/j.jes.2016.08.022. 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Accessed 9 Apr. 2020.Liu, Chao, et al. “The Effects of PH and Temperature on the Acetate Production and Microbial Community Compositions by Syngas Fermentation.” Fuel, vol. 224, 15 July 2018, pp. 537–544, www.sciencedirect.com/science/article/pii/S0016236118305337?casa_token=G8sFg1-lAY8AAAAA:vc4RvQIHqKFWs7GV4IYgsFbE19hHKG64wJa-VxHX2i4bWFeht1IvIjU2sKH_DqSD7k-vhD60_yE, 10.1016/j.fuel.2018.03.125. Accessed 1 June 2020.Liu, Shuang Ping, et al. “Bacterial Succession and the Dynamics of Volatile Compounds during the Fermentation of Chinese Rice Wine from Shaoxing Region.” World Journal of Microbiology and Biotechnology, vol. 31, no. 12, 22 Oct. 2015, pp. 1907–1921, 10.1007/s11274-015-1931-1. Accessed 19 May 2020.Luo, Haiping, et al. “Sulfate Reduction and Microbial Community of Autotrophic Biocathode in Response to Acidity.” Process Biochemistry, vol. 54, 1 Mar. 2017, pp. 120–127, www.sciencedirect.com/science/article/pii/S1359511316304482?casa_token=UsEoVQm9jdgAAAAA:K3y18r9pkFNEGbVXRhH9NYO2kn92gUTagQO4W9ne_7__4cREpqqMoMgpy3GTE4TGCsY2GmAOpUc, 10.1016/j.procbio.2016.12.025. Accessed 1 June 2020.Qin, Sijun, et al. “Forage Crops Alter Soil Bacterial and Fungal Communities in an Apple Orchard.” Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, vol. 66, no. 3, Oct. 2015, pp. 229–236, 10.1080/09064710.2015.1088569. Accessed 1 June 2020.Sun, Zhenli, et al. “Effects of BmCPV Infection on Silkworm Bombyx Mori Intestinal Bacteria.” PLOS ONE, vol. 11, no. 1, 8 Jan. 2016, p. e0146313, 10.1371/journal.pone.0146313. Accessed 1 June 2020.Wang, Honglei, et al. “Distribution Patterns of Nitrogen Micro-Cycle Functional Genes and Their Quantitative Coupling Relationships with Nitrogen Transformation Rates in a Biotrickling Filter.” Bioresource Technology, vol. 209, 1 June 2016, pp. 100–107, www.sciencedirect.com/science/article/pii/S0960852416302656?casa_token=cLS57Ina4g8AAAAA:JNbvH3JnbxWoT94kv786jXpCfJCgxl6uJBxSB3lvU4fyXMw4NUfFrn8wCpB6M-PtoKIRRIZqzKc, 10.1016/j.biortech.2016.02.119. Accessed 1 June 2020.Wang, Wen, et al. “Enhanced Fermentative Hydrogen Production from Cassava Stillage by Co-Digestion: The Effects of Different Co-Substrates.” International Journal of Hydrogen Energy, vol. 38, no. 17, 10 June 2013, pp. 6980–6988, www.sciencedirect.com/science/article/pii/S0360319913008525?casa_token=Yp5RO0rqkckAAAAA:TQ7GNvTa5EwFEgQpDmocPa28hj0Eq3LeR6esG9BZujRWKeb9Pl8UZnzwX4c64PKz2wRwp5I902E, 10.1016/j.ijhydene.2013.04.004. Accessed 1 June 2020.Zhang, Bin, et al. “Microbial Population Dynamics during Sludge Granulation in an Anaerobic–Aerobic Biological Phosphorus Removal System.” Bioresource Technology, vol. 102, no. 3, 1 Feb. 2011, pp. 2474–2480, www.sciencedirect.com/science/article/pii/S0960852410018195?casa_token=xABqGAsRc00AAAAA:3-gK60wIYoio7fBqwmlb8OM2y3fRILBwWlBaemc0pz6_fZFumW9c9gC0xMmpg_gXkfTJspCIl6o, 10.1016/j.biortech.2010.11.017. Accessed 1 June 2020.Zhi, Wei, et al. “Enhanced long-term nitrogen removal and its quantitative molecular mechanism in tidal flow constructed wetlands.” Environmental science & technology 49.7 (2015): 4575-4583.Zhou, Min, et al. “Evolution and Distribution of Resistance Genes and Bacterial Community in Water and Biofilm of a Simulated Fish-Duck Integrated Pond with Stress.” Chemosphere, vol. 245, 1 Apr. 2020, p. 125549, www.sciencedirect.com/science/article/pii/S0045653519327894?casa_token=fHNPjCLuBx0AAAAA:AgLxYXp1gUk5K-1Y2pEEZAU8BKUOB8t_P2NU_ZgPs7QGg70yoGzdaxrL-SvewC0Wsf7VBeflGec, 10.1016/j.chemosphere.2019.125549. Accessed 1 June 2020.—. “Spread of Resistance Genes from Duck Manure to Fish Intestine in Simulated Fish-Duck Pond and the Promotion of Cefotaxime and As.” Science of The Total Environment, vol. 731, 20 Aug. 2020, p. 138693, www.sciencedirect.com/science/article/pii/S0048969720322105, 10.1016/j.scitotenv.2020.138693. Accessed 1 June 2020.FormatMiniprep