首页 > Omega Bio-Tek > Omega Bio-Tek/E-Z 96® Total RNA Kit/4x96/R1034-01

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Omega Bio-Tek/E-Z 96® Total RNA Kit/4x96/R1034-01

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R1034-01

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Omega Bio-Tek

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OverviewThe E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5 x 105 cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilizing HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High-quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.SpecificationsFor Research Use Only. Not for use in diagnostic procedures.FeaturesSpecificationsDownstream ApplicationPCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purificationElution Volume50-75 μLStarting MaterialCulture cellsStarting Amountup to 5x105 cellsProcessing ModeManual, centrifugation/vacuumThroughput96 samples per runRNA Binding technologySilica membrane 96-wellProtocol and ResourcesProduct Documentation & LiteraturePROTOCOLR1034 E-Z 96® Total RNA KitSDSR1034 SDSSALES SHEETProduct Data#gap-298026278{padding-top:30px}Total purified RNA using the E-Z 96® Total RNA Kit Figure 1. Purified total RNA from 10 mg chicken liver was isolated with the E-Z 96® Total RNA Kit. Total RNA (500 ng/lane) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.#gap-766199547{padding-top:30px}Format96-well filter plate

Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.

Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.

2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.

Omega Bio-Tek公司的主要客户：孟山都、美国农业部等